Joy in the ministry 2000 and beyond

Convocation recorded at Concordia Seminary, March 19, 2003. Rev. William Ameiss, president of the Northern Illinois District, speaks about his personal experience in ministry as well as the importance of valuing the dreams of the young along with the experience of those who are older. He says you can have joy in challenge because of the promises of God. God is with us, and He is faithful

059. Mark 8:10-21

Chapel Sermon by William Ameiss from Mark 8:10-21 on Tuesday, March 18, 2003

Characterization of the alloantigens C, D and L of the chicken

Includes bibliographical references (pages [29]-32).M.S. (Master of Science

The <it>Salmonella </it>Pathogenicity Island (SPI) 1 contributes more than SPI2 to the colonization of the chicken by <it>Salmonella enterica </it>serovar Typhimurium

Abstract Background Salmonella enterica serovar Typhimurium (Typhimurium) is an important pathogen that infects a broad range of hosts. In humans, Typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. Typhimurium infection occurs mainly through the ingestion of contaminated food including poultry, pork, eggs, and milk. Chickens that are asymptomatic carriers of Typhimurium constitute a potential reservoir for infection. The type three secretion systems encoded by Salmonella pathogenicity islands (SPI) 1 and 2 are major virulence factors of Salmonella. However, only a few studies have investigated their role during the infection of chickens. Results We have taken a mixed infection approach to study the contribution of SPI1 and SPI2 to the colonization of the chicken by Typhimurium. We found that SPI1 contributes to colonization of both the cecum and spleen in the chicken. In contrast, SPI2 contributes to colonization of the spleen but not the cecum and, in the absence of SPI1, inhibits cecal colonization. Additionally, we show that the contribution of SPI1 in the spleen is greater than that of SPI2. These results are different from those observed during the infection of the mouse by Typhimurium where SPI2 is the major player during systemic colonization. Conclusion The co-infection model we used provides a sensitive assay that confirms the role of SPI1 and clarifies the role of SPI2 in the colonization of the chicken by Typhimurium.</p

Overexpression of Human DNA Topoisomerase IIα by Fusion to Enhanced Green Fluorescent Protein

DNA topoisomerase (topo) IIα is a major target for many anticancer agents. However, progress towards understanding how these agents interact with this enzyme in human cells and how resistance to these agents arises is greatly impeded by difficulties in expressing this gene. Here, we report on achieving a high level of expression of a full-length human topo IIα gene in human cells. We started with the topo IIα cDNA driven by a strong cytomegalovirus promoter and transiently transfected HeLa cells. Although topo IIα mRNA was consistently detected in transfected cells, no exogenous topo IIα protein was detected. By contrast, when the same cDNA was fused to an enhanced green fluorescent protein (EGFP), we detected a high level of expression at both mRNA and protein levels. The exogenous topo IIα was localized to cell nuclei as expected, indicating that the fusion protein is properly folded. Furthermore, overexpression of the EGFP-topo IIα fusion protein increased the sensitivity of the transfected cells to teniposide, suggesting that it functions as the endogenous counterpart. Thus, in addition to being used as a gene tag, the GFP fusion approach may be generally applicable for expressing genes, such as topo IIα, that are difficult to express by conventional methods