Syzygium jambolanum treatment improves survival in lethal sepsis induced in mice

<p>Abstract</p> <p>Background</p> <p>The leaves and the fruits from <it>Syzygium jambolanum </it>DC.(Myrtaceae), a plant known in Brazil as sweet olive or 'jambolão', have been used by native people to treat infectious diseases, diabetes, and stomachache. Since the bactericidal activity of <it>S. jambolanum </it>has been confirmed <it>in vitro</it>, the aim of this work was to evaluate the effect of the prophylactic treatment with <it>S. jambolanum </it>on the <it>in vivo </it>polymicrobial infection induced by cecal ligation and puncture (CLP) in mice.</p> <p>Methods</p> <p>C57Bl/6 mice were treated by the subcutaneous route with a hydroalcoholic extract from fresh leaves of <it>S. jambolanum </it>(HCE). After 6 h, a bacterial infection was induced in the peritoneum using the lethal CLP model. The mice were killed 12 h after the CLP induction to evaluate the cellular influx and local and systemic inflammatory mediators' production. Some animals were maintained alive to evaluate the survival rate.</p> <p>Results</p> <p>The prophylactic HCE treatment increased the mice survival, the neutrophil migration to infectious site, the spreading ability and the hydrogen peroxide release, but decreased the serum TNF and nitrite. Despite the increased migration and activation of peritoneal cells the HCE treatment did not decrease the number of CFU. The HCE treatment induced a significant decrease on the bone marrow cells number but did not alter the cell number of the spleen and lymph node.</p> <p>Conclusion</p> <p>We conclude that the treatment with <it>S. jambolanum </it>has a potent prophylactic anti-septic effect that is not associated to a direct microbicidal effect but it is associated to a recruitment of activated neutrophils to the infectious site and to a diminished systemic inflammatory response.</p

Measurement of the tt¯ production cross-section using eμ events with b-tagged jets in pp collisions at √s=7 and 8 TeV with the ATLAS detector

The inclusive top quark pair (tt¯tt¯) production cross-section σtt¯σtt¯ has been measured in proton–proton collisions at s√=7 TeVs=7 TeV and s√=8 TeVs=8 TeV with the ATLAS experiment at the LHC, using tt¯tt¯ events with an opposite-charge eμeμ pair in the final state. The measurement was performed with the 2011 7 TeV dataset corresponding to an integrated luminosity of 4.6 fb−1fb−1 and the 2012 8 TeV dataset of 20.3 fb−1fb−1. The numbers of events with exactly one and exactly two bb-tagged jets were counted and used to simultaneously determine σtt¯σtt¯ and the efficiency to reconstruct and bb-tag a jet from a top quark decay, thereby minimising the associated systematic uncertainties. The cross-section was measured to be: σtt¯σtt¯=182.9±3.1±4.2±3.6±3.3 pb (s√=7 TeV)and=242.4±1.7±5.5±7.5±4.2 pb (s√=8 TeV), σtt¯=182.9±3.1±4.2±3.6±3.3 pb (s=7 TeV)andσtt¯=242.4±1.7±5.5±7.5±4.2 pb (s=8 TeV), where the four uncertainties arise from data statistics, experimental and theoretical systematic effects, knowledge of the integrated luminosity and of the LHC beam energy. The results are consistent with recent theoretical QCD calculations at next-to-next-to-leading order. Fiducial measurements corresponding to the experimental acceptance of the leptons are also reported, together with the ratio of cross-sections measured at the two centre-of-mass energies. The inclusive cross-section results were used to determine the top quark pole mass via the dependence of the theoretically predicted cross-section on mpoletmtpole giving a result of mpoletmtpole=172.9+2.5−2.6=172.9−2.6+2.5 GeV. By looking for an excess of tt¯tt¯ production with respect to the QCD prediction, the results were also used to place limits on the pair-production of supersymmetric top squarks t~1t~1 with masses close to the top quark mass, decaying via t~1→tχ~01t~1→tχ~10 to predominantly right-handed top quarks and a light neutralino χ~01χ~10, the lightest supersymmetric particle. Top squarks with masses between the top quark mass and 177 GeV are excluded at the 95 % confidence level