Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling-5

<p><b>Copyright information:</b></p><p>Taken from "Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling"</p><p>http://www.biomedcentral.com/1471-2202/8/65</p><p>BMC Neuroscience 2007;8():65-65.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC1963332.</p><p></p>hybrid GFP (first chromosome) and homozygous for mutations were analyzed for the fluorescence in the wing. The rescue in background was analyzed as double heterozygous (). Flies were heat-shocked at day 3 and analyzed two days later. Positive control: MARCM system (chromosome recombination based on flipase under heat-shock promotor induces axonal marker -)

Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling-1

<p><b>Copyright information:</b></p><p>Taken from "Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling"</p><p>http://www.biomedcentral.com/1471-2202/8/65</p><p>BMC Neuroscience 2007;8():65-65.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC1963332.</p><p></p>re. In tube (C), homozygous mutants their genetic background give: T value 3.87, P = 0.001, degrees of freedom: 18 ; T value 7.6, P = 0.0001, degrees of freedom: 18 (); T value 4.36, P = 0.0004, degrees of freedom 18 (). The heterozygous mutants show a similar trend (n = 10). Statistics for mutants genetic background in tube C: T value 4, P = 0.001, degrees of freedom: 16 (and ); T value 1.98, P = 0.06, degrees of freedom: 16 (). (2) The experiment was also performed using a derivative protocol: one single pierced transparent tube was used with 1 ml grape juice in a saturated atmosphere. We observed progression of the accumulation from 2 to 24 hours. Values are mean ± SEM (n = 10). For at 24 hours: T value 1.8, P value: 0.085, degrees of freedom: 18 (3) Analysis of the mutant and the rescue (protocol as in (1)). The rescue (, along with , exposed to single acute heat shock at late third instar larva 3 day old adult stage, showed reverse phenotype (similar to control genetic background). Statistics for the mutant the rescue in tube C: T value 3.42, P = 0.003, degrees of freedom: 18 (n = 10)

Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling-0

<p><b>Copyright information:</b></p><p>Taken from "Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling"</p><p>http://www.biomedcentral.com/1471-2202/8/65</p><p>BMC Neuroscience 2007;8():65-65.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC1963332.</p><p></p>�l) was placed inside the three systems. The protocols are described in the Methods section. Briefly, tubes are painted black to eliminate any visual interference with exploration. One set of experiments is carried out in an aerated chamber (odour gradients are maintained), the other in grape juice-saturated atmosphere (see figure 4 and Methods section). One hundred flies were placed in the chamber and the flies inside the 3 tubes were counted 10 hours later. (A) tube with 50 μl, (B) 100 μl and (C) 300 μl. The mutants , and were analyzed along with genetic background and . Values are mean ± SEM (n = 10)

Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling-3

<p><b>Copyright information:</b></p><p>Taken from "Exploratory behaviour in NO-dependent cyclase mutants of shows defects in coincident neuronal signalling"</p><p>http://www.biomedcentral.com/1471-2202/8/65</p><p>BMC Neuroscience 2007;8():65-65.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC1963332.</p><p></p>nts. Mutants (20 flies, 5 days old, red eyed, females to avoid pheromone interference) were released in the chamber without disturbing the aggregated spot (aggregates stable, probably because of altered vision). Simultaneously, a second fresh spot was placed inside. Here, the graph represents the time course of the ratio of aggregated flies (or ) on the pre-aggregated spot the fresh one. Values are mean ± SEM (n = 10). The mutant against control gave T value: 3.38, P = 0.003 and degrees of freedom: 18. Heat shock rescue was carried out as in figure 1. () The control of flies aggregated the total in the chamber is represented for the strains tested above. We saw no significant differences between the strains. The apparatus was the same than in the figure 5 and 6