Staphylococcus aureus nasal carriage among healthcare workers in Kisangani, the Democratic Republic of the Congo

Methicillin-resistant Staphylococcus aureus (MRSA) is a global health concern, but there are few data from Central Africa. The objective of our study was to characterise S. aureus colonisation isolates from healthcare-exposed professionals in the Democratic Republic of the Congo (DRC). Healthcare workers and medical students (n = 380) in Kisangani, DRC were screened for S. aureus nasal carriage in a single-centre cross-sectional study in the University Hospital of Kisangani. The isolates were identified and characterised using phenotypic and genotypic methods. The nasal carriage rate of S. aureus was 16.6 % and 10 out of 63 isolates (15.9 %) were MRSA. We found 28 different spa types. Most MRSA isolates belonged to ST8-spa t1476-SCCmec V. The majority of MRSA were multidrug-resistant to non-beta-lactam antibiotics. Overall, 28.5 % of S. aureus carried Panton–Valentine leucocidin (PVL)-encoding genes (all methicillin-sensitive) and 17.5 % carried toxic shock syndrome toxin-1 (TSST-1)-encoding genes. The finding of MRSA carriage among healthcare workers in a setting with limited access to diagnostic microbiology and appropriate therapy calls for improved education on infection control practices and supports the introduction of surveillance programmes.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Antibiotic Prescribing in DR Congo: A Knowledge, Attitude and Practice Survey among Medical Doctors and Students

<div><p>Objectives</p><p>Antibiotic resistance (ABR) particularly hits resource poor countries, and is fuelled by irrational antibiotic (AB) prescribing. We surveyed knowledge, attitudes and practices of AB prescribing among medical students and doctors in Kisangani, DR Congo.</p> <p>Methods</p><p>Self-administered questionnaires.</p> <p>Results</p><p>A total of 184 questionnaires were completed (response rate 94.4%). Knowledge about AB was low (mean score 4.9/8 points), as was the estimation of local resistance rates of <i>S.</i> Typhi and <i>Klebsiella</i> spp.(correct by 42.5% and 6.9% of respondents respectively). ABR was recognized as a problem though less in their own practice (67.4%) than nation- or worldwide (92.9% and 85.5%, p<.0001). Confidence in AB prescribing was high (88.6%) and students consulted more frequently colleagues than medical doctors when prescribing (25.4% versus 11.6%, p  = 0.19). Sources of AB prescribing included pharmaceutical companies (73.9%), antibiotic guidelines (66.3%), university courses (63.6%), internet-sites (45.7%) and WHO guidelines (26.6%). Only 30.4% and 16.3% respondents perceived AB procured through the central procurement and local pharmacies as of good quality. Local AB guidelines and courses about AB prescribing are welcomed (73.4% and 98.8% respectively).</p> <p>Conclusions</p><p>This data shows the need for interventions that support rational AB prescribing.</p> </div

Knowledge questions and results.

*<p>only questions 1–8 were included in the score;</p>**<p>trimethoprim/sulphamethoxazole.</p

Sources of information about AB and AB prescribing used in the month prior to the survey, categorized for last year medical students versus medical doctors.

<p>Sources of information about AB and AB prescribing used in the month prior to the survey, categorized for last year medical students versus medical doctors.</p

Perception of Antibiotic resistance as a problem.

<p>Perception of Antibiotic resistance as a problem.</p

Map of the study site.

<p>Map of the study site.</p

Factors contributing to antibiotic resistance in DRC.

*<p>Don’t Know.</p>**<p>No Data.</p