MEASUREMENT OF CORTISOL AND CORTISONE IN HUMAN SALIVA BY UPLC-MS/MS

Objective: To develop and validate a simple and rapid assay for simultaneous measurement of cortisol and cortisone in human saliva by ultra-performance liquid chromatography-tandem mass spectrometry. Methods: Chromatographic analysis was performed on an Atlantis dC18 column (2.1 x 100 mm, 3 µm) using a mobile phase consisting of acetonitrile and 2 mmol ammonium-acetate (50:50, v; v) that was delivered at a flow rate of 0.3 ml/min. The eluents were monitored using electrospray ionization in the positive ion mode set at transition set of mass-to-charge (m/z): 363.11 → 121.00, 361.18 → 163.11, and 367.19 → 121.24 for cortisol, cortisone and internal standard (IS), respectively the method was validated for linearity, accuracy, precision, and recovery, according to international guidelines. Results: The retention times of cortisol, cortisone and internal were about 1.38, 1.43 and 1.38 min, respectively. Cortisol level and cortisone level relationship to the ratio of their respective peak-area to IS’s peak-area was linear (range of 0.5-100 ng/ml). Coefficients of variation and inaccuracy were, ≤9.9% and-0.3 to 6.9 for cortisol and ≤8.4 and-1.5 to 4.8 for cortisone, respectively. Extraction recoveries for cortisol, cortisone, and the IS were 90%, 94%, and 98%, respectively. Cortisol and cortisone stability was evaluated in processed saliva samples (stored at room temperature for 24 h) and unprocessed saliva samples (stored at room temperature for 24 h or at-20 °C for 10 w) and after 3 freeze-thaw cycles was ≥ 86%. Conclusion: The proposed method is simple, precise, and accurate for the rapid simultaneous measurement of cortisol and cortisone levels in saliva. The assay was successfully applied to determine levels of cortisol and cortisone in human saliva samples obtained from healthy volunteers

Kajian Pelayanan Unit Pelaksana Teknis Daerah Pelabuhan Perikanan Pantai Lampulo terhadap Kepuasan Nelayan

Penelitian ini bertujuan untuk mengetahui: 1) Profil dan mekanisme kerja di Unit PelaksanaTeknis Daerah (UPTD) Pelabuhan Perikanan Pantai (PPP) Lampulo Banda Aceh, 2) Pengaruh dari variabel kualitas pelayanan yang terdiri dari (bukti fisik, jaminan dan kepedulian) terhadap kepuasan nelayan. Penelitian ini dilakukan pada bulan April 2016. Metode yang digunakan dalam penelitian ini adalah metode survei dan wawancara dengan analisis deskriptif kualitatif dan kuantitatif. Teknik pengambilan sampel yaitu dengan purposive sampling dengan respondennya adalah nelayan. Analisis data menggunakanChi-Squaredengan programSPSS. Hasil penelitian menunjukkan bahwa berdasarkan 3 variabel yang diuji (bukti fisik, jaminan dan kepedulian), yang berpengaruh signifikan terhadap kepuasan nelayan adalah variabel kepedulian dengan nilai variabel kepedulian p-0,0250,05, p-0,194>0,05. The conclusion has reliability varies significantly influenced satisfaction fishermen

Pengaruh Brand Image dan Product Quality terhadap Brand Love dan Brand Loyalty pada Pengguna Mobil Honda Jazz di Kota Pekanbaru

This research aimed to analysze Brand Image and Product Quality to Brand Love and Branf Loyalty Honda Jazz users in Pekanbaru City. The object of this research was Honda Jazz users in Pekanbaru City. This research consists of four variables: two dependent variable and two independent variables. They are Brand Loyalty (Y2), Brand Love (Y1), Product Quality (X2), and Brand Image (X1). This research was conducted using a sample of 170 people. This research used purposive sampling, based on respondents who have Honda Jazz as personal car, aged 15 years and over. Data are taken by giving respondents structural questionaires. In terms of data analysis, this research used Structural Equation Modeling (SEM) with AMOS version 22. Based on the hypothesis test conducted, research shows that Brand Image has significant and positive effect toward Brand Love, Product Quality has significant and positive effect toward Brand Love. Brand Image has significant and positive effect toward Brand Loyalty, Product Quality has significant and positive effect toward Brand Loyalty and Brand Love has significant and positive effect toward Brand Loyalty

Peranan Penyidik Pegawai Negeri Sipil (Ppns) Perpajakan dan Penyidik Polri dalam Penanganan Tindak Pidana Perpajakan

The tax is an important thing as welfare state as one of income source for the increasing of social welfare in a state. Indonesia is one of nation that put tax as one of state income source although it has not yet put tax as one of increasing of society welfare. In the law enforcement process include the tax law enforcement, it always face to criminal justice system. This system is one of system to eradicate the crime in a society. Crimnal justice system has any components, i.e. police, attorney, court, and correctional instituation. In addition to the component of the criminal justice system, there is one specific component for the case of tax, i.e. the civil servant investigator (PPNS) who has responsibility to do the investigation if there is a crime in tax to support the public attorney in handle the tax case. The role of PPNS as instuation out of Police aims to help the task of police in to the investigation that determined in the Crime Procesure Law and Act No. 2 of 2002 concerning to the Police of republic of Indonesia. PPNS or Police must helpot one to the others especially in provide the required information about the crime case to support the crime investigation actually and completely to avoid the intersect of authority in do the investigation that requires the coordination and supervision between the related instituation in enforcement, and socialization of the rule related to the authority in any investigation and to obtain the understanding about the task and authority for each instituation. Through this socialization it will eliminate the gap between the instituation and realize the complete instituation

Pertanggungjawaban Pidana Illegal Logging (Pembalakan Liar) sebagai Kejahatan Kehutanan Berdasarkan Undang-undang No. 41 Tahun 1999 Tentang Kehutanan dan Undang-undang No. 18 Tahun 2013 Tentang Pencegahan dan Pemberantasan Perusakan Hutan

Continuous destruction of natural forest has caused the decrease in the forest area. The problem of criminal act in forestry, especially illegal logging, is a very complicated problem to be coped with since the criminal act is very serious, well-organized, and has inter-state range. The people's lack of knowledge in the importance of forest for human benefit and the weakness of law has caused the complexity of any effort to handle illegal logging. Law No. 14/1999 on Forestry has not yet formulated the definition of illegal logging so that it causes multi-interpretation. Besides that, there are many weaknesses in the previous legal provisions which cause the prevention and the eradication become complicated. Therefore, Law No. 18/2013 on the Prevention and the Eradication was established. The result of the research showed that the criminal elements of illegal logging were found in Article 12 points a, b, c, d, e, f, and g, and in Article 19 points a, b, c, d, and f of Law No. 18/2013. Criminal responsibility of the perpetrators of illegal logging individually and corporately, either intentionally or because of negligence has been formulated in Articles 82 up to 85, in Article 94, and in Article 98. The reasons for annulling the criminal act is found in Article 11, paragraph (3) and in Article 13, paragraph (2) of Law No. 18/2013

Pertanggungjawaban Korporasi dalam Tindak Pidana Kehutanan (Studi Putusan Kasasi Mahkamah Agung RI Nomor 2642 K/pid/2006)

The corporation case which occurs in North Sumatera is a forestry criminal act committed by Darianus Lungguk Sitorus, the owner of PT Torganda and PT Torus Ganda. He did forestry criminal act in the area of Hutan Padang Lawas through his corporation. As the perpetrator who did the criminal act in this case, he was imprisoned by the Ruling of the Supreme Court No 2642 K/Pid/2006 for 8 (eight) year imprisonment plus incurred a fine of Rp. 5,000,000,000 (five billion rupiahs) plus confiscation of 47,000 (forty seven thousand) hectares of oil palm plantation, located in the area of Padang Lawas by the Department of Forestry.The problems of the research were the development of corporation responsibility for forestry criminal act in Indonesia and whether judge's legal consideration on corporation criminal responsibility in the Cassation Ruling of the Supreme Court No 2642 K/Pid/2006 had met the theory of criminal responsibility. The research used judicial prescriptive method with legal provision, case, and comparative approaches, using primary, secondary, and tertiary legal materials which were analyzed interpretatively. The result of the research showed that corporation criminal responsibility in forestry criminal act in Indonesia is developing in ius constitutum which indicated by the development of the development of legal subject and corporation criminal responsibility. Law No. 41/1999 on Forestry which has gone through the development of corporation criminal responsibility in Law No. 18/2013 on Prevention from and Eradication of Forest Damage, and Cassation Ruling of the Supreme Court No. 2642 K/Pid/2006 has directly used the theory of corporation criminal responsibility. As a directing mind, Darianus Lungguk Sitorus had a corporation inner-self in ordering to commit (doen plegen) forestry criminal act

QUANTITATION OF AMLODIPINE IN HUMAN PLASMA BY LCMS/MS ASSAY

­­­­­­­­­­­­­­­­­Objective: To develop and validate a simple, precise, and rapid liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for quantification of amlodipine in human plasma.Methods: Chromatographic analysis was performed on Atlantis dC18 column (2.1 x 100 mm, 3 µm) with a mobile phase consisting of acetonitrile and 10 mM formic acid (80:20, v: v) that was delivered at a flow rate of 0.3 ml/min. The eluents were monitored using electrospray ionization in the positive ion mode set at transition 409 → 238.4 and 254.3 → 43.9 for amlodipine and tizanidine hydrochloride (IS), respectively. The method was validated for linearity, accuracy, precision, and recovery as per US-FDA guidelines.Results: The retention times of amlodipine and tizanidine (IS) were 1.26 and 1.22 respectively. The relationship between amlodipine concentration and peak height ratio of amlodipine to the IS was linear (R2³ 0.9868) in the range of 0.2–20 ng/ml, and the intra-and inter-day coefficient of variations and bias were ≤14.4% and ≤13.6% and ≤13.7% and ≤11.2%, respectively.Conclusion: The proposed method is simple, precise, and accurate for rapid measurement of amlodipine level using 0.5 ml human plasma. Further, the assay was successfully applied to determine amlodipine level in human plasma samples obtained from a healthy volunteer