33 research outputs found
Stability of isoenzyme and kinetoplast DNA (k-DNA) patterns in successively cloned Trypanosoma cruzi populations
Evaluation of the bactericidal and trypanocidal activities of triterpenes isolated from the leaves, stems, and flowers of Lychnophora pinaster
NADPH Phagocyte Oxidase Knockout Mice Control Trypanosoma cruzi Proliferation, but Develop Circulatory Collapse and Succumb to Infection
âąNO is considered to be a key macrophage-derived cytotoxic effector during Trypanosoma cruzi infection. On the other hand, the microbicidal properties of reactive oxygen species (ROS) are well recognized, but little importance has been attributed to them during in vivo infection with T. cruzi. In order to investigate the role of ROS in T. cruzi infection, mice deficient in NADPH phagocyte oxidase (gp91phoxâ/â or phox KO) were infected with Y strain of T. cruzi and the course of infection was followed. phox KO mice had similar parasitemia, similar tissue parasitism and similar levels of IFN-Îł and TNF in serum and spleen cell culture supernatants, when compared to wild-type controls. However, all phox KO mice succumbed to infection between day 15 and 21 after inoculation with the parasite, while 60% of wild-type mice were alive 50 days after infection. Further investigation demonstrated increased serum levels of nitrite and nitrate (NOx) at day 15 of infection in phox KO animals, associated with a drop in blood pressure. Treatment with a NOS2 inhibitor corrected the blood pressure, implicating NOS2 in this phenomenon. We postulate that superoxide reacts with âąNO in vivo, preventing blood pressure drops in wild type mice. Hence, whilst superoxide from phagocytes did not play a critical role in parasite control in the phox KO animals, its production would have an important protective effect against blood pressure decline during infection with T. cruzi
In vitro cytotoxic, antifungal, trypanocidal and leishmanicidal activities of acetogenins isolated from Annona cornifolia A. St. -Hil. (Annonaceae)
A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
Uso do Random Amplified Polymorphic DNA (RAPD) no estudo populacional do Triatoma brasiliensis Neiva, 1911
Para o estudo de variabilidade genĂ©tica em Triatoma brasiliensis, o principal vetor da doença de Chagas no Nordeste brasileiro, espĂ©cimes de trĂȘs diferentes populaçÔes intradomiciliares foram analisados. RegiĂ”es do DNA genĂŽmico foram amplificadas utilizando dois iniciadores randĂŽmicos atravĂ©s da tĂ©cnica de RAPD (Random Amplified Polymorphic DNA), visualizados em gĂ©is de poliacrilamida corados pela prata. Os perfis originados se mostraram bastante homogĂȘneos quando comparados intrapopulacionalmente. PopulaçÔes capturadas em duas regiĂ”es diferentes do Estado do CearĂĄ tambĂ©m apresentaram homogeneidade entre si, mas, quando comparadas com a população proveniente do PiauĂ, foi possĂvel diferenciĂĄ-las. Esses resultados, preliminares, indicam que o RAPD pode ser usado com sucesso nos estudos de variabilidade em triatomĂneos, bem como sugerem a existĂȘncia de variabilidade entre diferentes populaçÔes de T. brasiliensis pertencentes a uma mesma subespĂ©cie