4,786 research outputs found
Position estimation delays in signal injection-based sensorless PMSM drives
The causes of position estimation delays and their effects on the sensorless control of permanent magnet synchronous motor drives are investigated. The position of a permanent magnet synchronous machine is estimated via the injection of high frequency voltage signals. The delays under investigation are due to the digital implementation of the control algorithm and to the digital filters adopted for decoupling the inspection signals from the fundamental components of the stator current measures. If not correctly modeled and compensated, such delays can reduce the performance of the control scheme. Experimental results are provided, proving the accuracy of the modeling approach and the effectiveness of the related compensation strateg
Parametric coupling between macroscopic quantum resonators
Time-dependent linear coupling between macroscopic quantum resonator modes
generates both a parametric amplification also known as a {}"squeezing
operation" and a beam splitter operation, analogous to quantum optical systems.
These operations, when applied properly, can robustly generate entanglement and
squeezing for the quantum resonator modes. Here, we present such coupling
schemes between a nanomechanical resonator and a superconducting electrical
resonator using applied microwave voltages as well as between two
superconducting lumped-element electrical resonators using a r.f.
SQUID-mediated tunable coupler. By calculating the logarithmic negativity of
the partially transposed density matrix, we quantitatively study the
entanglement generated at finite temperatures. We also show that
characterization of the nanomechanical resonator state after the quantum
operations can be achieved by detecting the electrical resonator only. Thus,
one of the electrical resonator modes can act as a probe to measure the
entanglement of the coupled systems and the degree of squeezing for the other
resonator mode.Comment: 15 pages, 4 figures, submitte
Gene expression of stem cells at different stages of ontological human development
Objectives
To compare multipotent mesenchymal stem cells (MSCs) obtained from chorionic villi (CV), amniotic fluid (AF) and placenta, with regard to their phenotype and gene expression, in order to understand if MSCs derived from different extra-embryonic tissues, at different stages of human ontological development, present distinct stemness characteristics.
Study design
MSCs obtained from 30 samples of CV, 30 of AF and 10 placentas (obtained from elective caesarean sections) were compared. MSCs at second confluence cultures were characterized by immunophenotypic analysis with flow cytometry using FACS CANTO II. The expression of the genes Oct-4 (Octamer-binding transcription factor 4, also known as POU5F1), Sox-2 (SRY box-containing factor 2), Nanog, Rex-1 (Zfp-42) and Pax-6 (Paired Box Protein-6), was analyzed. Real-time quantitative PCR was performed by ABI Prism 7700, after RNA isolation and retro-transcription in cDNA. Statistical analysis was performed using non-parametric test Kruskal–Wallis (XLSTAT 2011) and confirmed by REST software, to estimate fold changes between samples. Each gene was defined differentially expressed if p-value was <0.05.
Results
Cells from all samples were negative for haematopoietic antigens CD45, CD34, CD117 and CD33 and positive for the typical MSCs antigens CD13, CD73 and CD90. Nevertheless, MSCs from AF and placentas showed different fluorescence intensity, reflecting the heterogeneity of these tissues. The gene expression of OCT-4, SOX-2, NANOG was not significantly different among the three groups. In AF, REX-1 and PAX-6 showed a higher expression in comparison to CV.
Conclusions
MSCs of different extra-embryonic tissues showed no differences in immunophenotype when collected from second confluence cultures. The expression of OCT-4, NANOG and SOX-2 was not significantly different, demonstrating that all fetal sources are suitable for obtaining MSCs. These results open new possibilities for the clinical use of MSCs derived from easily accessible sources, in order to develop new protocols for clinical and experimental research
Probing High Frequency Noise with Macroscopic Resonant Tunneling
We have developed a method for extracting the high-frequency noise spectral
density of an rf-SQUID flux qubit from macroscopic resonant tunneling (MRT)
rate measurements. The extracted noise spectral density is consistent with that
of an ohmic environment up to frequencies ~ 4 GHz. We have also derived an
expression for the MRT lineshape expected for a noise spectral density
consisting of such a broadband ohmic component and an additional strongly
peaked low-frequency component. This hybrid model provides an excellent fit to
experimental data across a range of tunneling amplitudes and temperatures
Decoherence, Autler-Townes effect, and dark states in two-tone driving of a three-level superconducting system
We present a detailed theoretical analysis of a multi-level quantum system
coupled to two radiation fields and subject to decoherence. We concentrate on
an effect known from quantum optics as the Autler-Townes splitting, which has
been recently demonstrated experimentally [M. A. Sillanpaa et al., Phys. Rev.
Lett. 103, 193601 (2009)] in a superconducting phase qubit. In the three-level
approximation, we derive analytical solutions and describe how they can be used
to extract the decoherence rates and to account for the measurement data.
Better agreement with the experiment can be obtained by extending this model to
five levels. Finally, we investigate the stationary states created in the
experiment and show that their structure is close to that of dark states.Comment: 16 pages, 8 figure
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