The Effect of Ginger Active Component (Zerumbone) on Human Cancer Cells.

Zerumbone, a sesquiterpene extracted from rhizomes of ginger (zingiber zerumbet Smith) is reported to have anti-proliferative activities and can induce toxicity in human cancer cells. However, its molecular mechanisms' are still poorly understood. In this study, in vitro antioxidant (DPPH, H2O2, Fe2+ chelating and reducing power), apoptotic and anti-proliferative activities of zerumbone were investigated in human cancer cells. The specific objective was to identify whether zerumbone-induced cell death occurs through apoptosis, autophagy, necrosis or another fmID of cell death by undeliaking morphological and biochemical characterisation. Human cancer cell line (Caco-2, Huh-7 and EA.hy926) viability and activity with time and in the presence of different concentrations of zerumbone were investigated using LDH. In addition, characterisation of cell death induced by different concentrations of zerumbone including changes in cell sizerphosphatidylserine externalization, caspase activation and P ARP-l involvement were studied. The results showed that cancer cell death occulTed in the absence of DNA fragmentation and caspase activation at (5 g/μml). Additionally, cancer cell death was characterised by cell shrinkage and an absence of necrotic cell death pathway. Anti-proliferative activity of zerumbone (5 and 10 Ilg/ml) on human cancer cells was also investigated by changes in the DNA content using flow cytometry

The Effect of Ginger Active Component (Zerumbone) on Human Cancer Cells.

Zerumbone, a sesquiterpene extracted from rhizomes of ginger (zingiber zerumbet Smith) is reported to have anti-proliferative activities and can induce toxicity in human cancer cells. However, its molecular mechanisms' are still poorly understood. In this study, in vitro antioxidant (DPPH, H2O2, Fe2+ chelating and reducing power), apoptotic and anti-proliferative activities of zerumbone were investigated in human cancer cells. The specific objective was to identify whether zerumbone-induced cell death occurs through apoptosis, autophagy, necrosis or another fmID of cell death by undeliaking morphological and biochemical characterisation. Human cancer cell line (Caco-2, Huh-7 and EA.hy926) viability and activity with time and in the presence of different concentrations of zerumbone were investigated using LDH. In addition, characterisation of cell death induced by different concentrations of zerumbone including changes in cell sizerphosphatidylserine externalization, caspase activation and P ARP-l involvement were studied. The results showed that cancer cell death occulTed in the absence of DNA fragmentation and caspase activation at (5 g/μml). Additionally, cancer cell death was characterised by cell shrinkage and an absence of necrotic cell death pathway. Anti-proliferative activity of zerumbone (5 and 10 Ilg/ml) on human cancer cells was also investigated by changes in the DNA content using flow cytometry

Pyrolysis and thermogravimetric study to elucidate the bioenergy potential of novel feedstock produced on poor soils while keeping the environmental sustainability intact

This work focused on exploring the bioenergy potential of biomass produced on salt-affected soils by growing two types of grasses, namely Parthenium hysterophorus (carrot grass) and Pennesetum benthiumo (mott grass), without using fertilizers or pesticides. The whole plant biomass of both grasses was pyrolyzed at three heating rates (10, 30, and 50 °C min−1) in a joined Thermogravimetry–Differential Scanning Calorimetry (TGA–DSC) analyzer under an inert (nitrogen) environment. The pyrolysis of both grasses was shown to occur in a three-stage process, while most of the thermal transformation occurred at the temperature range of 240–400 °C. The pyrolytic behavior was assessed by estimating the kinetic parameters, using the isoconversional models of Kissenger–Akahira–Sunose and Ozawa–Flynn–Wall. The average values of the activation energy of carrot and mott grasses were shown to be 267 kJ mol−1 (R2 ≥ 0.98) and 188 kJ mol−1 (R2 ≥ 0.98), indicating the suitability of both grasses for co-pyrolysis. Whereas, the difference in the values of enthalpy change and the activation energy was shown to be <~5 kJ mol−1 at each fractional point, which indicated that the product formation was being favored. Moreover, the high heating values of carrot grass (18.25 MJ kg−1) and mott grass (18.63 MJ kg−1) have shown a remarkable bioenergy potential and suitability of co-pyrolysis for both grasses. This study will lead to establishing an energy-efficient and cost-effective process for the thermal transformation of biomass to bioenergy