Dental Treatment under General Anesthesia in Healthy and Medically Compromised/Developmentally Disabled Children: A Comparative Study

Aim: To compare the type, number of procedures and working time of dental treatment provided under dental general anesthesia (DGA) in healthy and medically compromised/developmentally disabled children (MCDD children). Design: This cross-sectional prospective study involved 80 children divided into two groups of 40 children each. Group 1 consisted of healthy and Group 2 consisted of MCDD children. Results: Healthy children needed more working time than MCDD children, the means being 161±7.9 and 84±5.7 minutes, respectively (P= 0.0001). Operative dentistry and endodontic treatments showed a significant statistical difference (P= 0.0001). The means of procedures were 17±5.0 for healthy children and 11±4.8 for MCDD children (P= 0.0001). Conclusions: Healthy children needed more extensive dental treatment than MCDD children under DGA. The information from this sample of Mexican children could be used as reference for determining trends both within a facility as well as in comparing facilities in cross-population studies

Oxygen abundances in unevolved metal-poor stars from near-UV OH lines

We have performed a detailed oxygen abundance analysis of 23 metal-poor (-3.0<[Fe/H]<-0.3) unevolved halo stars and one giant through the OH bands in the near UV, using high-resolution echelle spectra. Oxygen is found to be overabundant with respect to iron in these stars, with the [O/Fe] ratio increasing from 0.6 to 1 between [Fe/H]=-1.5 and -3.0. The behavior of the oxygen overabundance with respect to [Fe/H] is similar to that seen in previous works based on OI IR triplet data (Abia and Rebolo 1989; Tomkin et al. 1992; Cavallo, Pilachowski, and Rebolo 1997). Contrary to the previously accepted picture, our oxygen abundances, derived from low-excitation OH lines, agree well with those derived from high-excitation lines of the triplet. For nine stars in common with Tomkin et al. we obtain a mean difference of 0.00+/-0.11 dex with respect to the abundances determined from the triplet using the same stellar parameters and model photospheres. For four stars in our sample we have found measurements of the [OI] 6300 A line in the literature, from which we derive oxygen abundances consistent (average difference 0.09 dex) with those based on OH lines, showing that the long standing controversy between oxygen abundances from forbidden and permitted lines in metal-poor unevolved stars can be resolved. Our new oxygen abundances show a smooth extension of the Edvardsson et al.'s (1993) [O/Fe] versus metallicity curve to much lower abundances, with a slope -0.31+/- 0.11 (taking into account the error bars in both oxygen abundances and metallicities) in the range -3<[Fe/H]<-1.Comment: 17 pages, 8 figures, 3 table

Boron in Very Metal-Poor Stars

We have observed the B I 2497 A line to derive the boron abundances of two very metal-poor stars selected to help in tracing the origin and evolution of this element in the early Galaxy: BD +23 3130 and HD 84937. The observations were conducted using the Goddard High Resolution Spectrograph on board the Hubble Space Telescope. A very detailed abundance analysis via spectral synthesis has been carried out for these two stars, as well as for two other metal-poor objects with published spectra, using both Kurucz and OSMARCS model photospheres, and taking into account consistently the NLTE effects on the line formation. We have also re-assessed all published boron abundances of old disk and halo unevolved stars. Our analysis shows that the combination of high effective temperature (Teff > 6000 K, for which boron is mainly ionized) and low metallicity ([Fe/H]<-1) makes it difficult to obtain accurate estimates of boron abundances from the B I 2497 A line. This is the case of HD 84937 and three other published objects (including two stars with [Fe/H] ~ -3), for which only upper limits can be established. BD +23 3130, with [Fe/H] ~ -2.9 and logN(B)_NLTE=0.05+/-0.30, appears then as the most metal-poor star for which a firm measurement of the boron abundance presently exists. The evolution of the boron abundance with metallicity that emerges from the seven remaining stars with Teff < 6000 K and [Fe/H]<-1, for which beryllium abundances were derived using the same stellar parameters, shows a linear increase with a slope ~ 1. Furthermore, the B/Be ratio found is constant at a value ~ 20 for stars in the range -3<[Fe/H]<-1. These results point to spallation reactions of ambient protons and alpha particles with energetic particles enriched in CNO as the origin of boron and beryllium in halo stars.Comment: 38 pages, 11 Encapsulated Postscript figures (included), uses aaspp4.sty. Accepted for publication in The Astrophysical Journal. The preprint is also available at: http://www.iac.es/publicaciones/preprints.htm

Near-Infrared Polarimetric Adaptive Optics Observations of NGC 1068: A torus created by a hydromagnetic outflow wind

We present J' and K' imaging linear polarimetric adaptive optics observations of NGC 1068 using MMT-Pol on the 6.5-m MMT. These observations allow us to study the torus from a magnetohydrodynamical (MHD) framework. In a 0.5" (30 pc) aperture at K', we find that polarisation arising from the passage of radiation from the inner edge of the torus through magnetically aligned dust grains in the clumps is the dominant polarisation mechanism, with an intrinsic polarisation of 7.0%$\pm$2.2%. This result yields a torus magnetic field strength in the range of 4$-$82 mG through paramagnetic alignment, and 139$^{+11}_{-20}$ mG through the Chandrasekhar-Fermi method. The measured position angle (P.A.) of polarisation at K$'$ is found to be similar to the P.A. of the obscuring dusty component at few parsec scales using infrared interferometric techniques. We show that the constant component of the magnetic field is responsible for the alignment of the dust grains, and aligned with the torus axis onto the plane of the sky. Adopting this magnetic field configuration and the physical conditions of the clumps in the MHD outflow wind model, we estimate a mass outflow rate $\le$0.17 M$_{\odot}$ yr$^{-1}$ at 0.4 pc from the central engine for those clumps showing near-infrared dichroism. The models used were able to create the torus in a timescale of $\geq$10$^{5}$ yr with a rotational velocity of $\leq$1228 km s$^{-1}$ at 0.4 pc. We conclude that the evolution, morphology and kinematics of the torus in NGC 1068 can be explained within a MHD framework.Comment: 14 pages, 4 figures, Accepted by MNRA

On the difference of torus geometry between hidden and non-hidden broad line active galactic nuclei

We present results from the fitting of infrared (IR) spectral energy distributions of 21 active galactic nuclei (AGN) with clumpy torus models. We compiled high spatial resolution ($\sim 0.3$--$0.7$ arcsec) mid-IR $N$-band spectroscopy, $Q$-band imaging and nuclear near- and mid-IR photometry from the literature. Combining these nuclear near- and mid-IR observations, far-IR photometry and clumpy torus models, enables us to put constraints on the torus properties and geometry. We divide the sample into three types according to the broad line region (BLR) properties; type-1s, type-2s with scattered or hidden broad line region (HBLR) previously observed, and type-2s without any published HBLR signature (NHBLR). Comparing the torus model parameters gives us the first quantitative torus geometrical view for each subgroup. We find that NHBLR AGN have smaller torus opening angles and larger covering factors than those of HBLR AGN. This suggests that the chance to observe scattered (polarized) flux from the BLR in NHBLR could be reduced by the dual effects of (a) less scattering medium due to the reduced scattering volume given the small torus opening angle and (b) the increased torus obscuration between the observer and the scattering region. These effects give a reasonable explanation for the lack of observed HBLR in some type-2 AGN.Comment: 13 pages, 5 figures, accepted for publication in Ap

Ribavirin as a First Treatment Approach for Hepatitis E Virus Infection in Transplant Recipient Patients

The hepatitis E virus (HEV) is the major cause of acute hepatitis of viral origin worldwide. Despite its usual course as an asymptomatic self-limited hepatitis, there are highly susceptible populations, such as those with underlying immunosuppression, which could develop chronic hepatitis. In this situation, implementation of therapy is mandatory in the sense to facilitate viral clearance. Currently, there are no specific drugs approved for HEV infection, but ribavirin (RBV), the drug of choice, is used for off-label treatment. Here, we present two cases of chronic HEV infection in transplant patients, reviewing and discussing the therapeutic approach available in the literature. The use of RBV for the treatment of an HEV infection in organ transplant patients seems to be effective. The recommendation of 12 weeks of therapy is adequate in terms of efficacy. Nevertheless, there are important issues that urgently need to be assessed, such as optimal duration of therapy and drug dosage

Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress

In recent years evolutionary ecologists have become increasingly interested in the effects of reactive oxygen species (ROS) on the life-histories of animals. ROS levels have mostly been inferred indirectly due to the limitations of estimating ROS from in vitro methods. However, measuring ROS (hydrogen peroxide, H2O2) content in vivo is now possible using the MitoB probe. Here, we extend and refine the MitoB method to make it suitable for ecological studies of oxidative stress using the brown trout Salmo trutta as model. The MitoB method allows an evaluation of H2O2 levels in living organisms over a timescale from hours to days. The method is flexible with regard to the duration of exposure and initial concentration of the MitoB probe, and there is no transfer of the MitoB probe between fish. H2O2 levels were consistent across subsamples of the same liver but differed between muscle subsamples and between tissues of the same animal. The MitoB method provides a convenient method for measuring ROS levels in living animals over a significant period of time. Given its wide range of possible applications, it opens the opportunity to study the role of ROS in mediating life history trade-offs in ecological settings

Plasticity and dystonia: a hypothesis shrouded in variability.

Studying plasticity mechanisms with Professor John Rothwell was a shared highlight of our careers. In this article, we discuss non-invasive brain stimulation techniques which aim to induce and quantify plasticity, the mechanisms and nature of their inherent variability and use such observations to review the idea that excessive and abnormal plasticity is a pathophysiological substrate of dystonia. We have tried to define the tone of our review by a couple of Professor John Rothwell's many inspiring characteristics; his endless curiosity to refine knowledge and disease models by scientific exploration and his wise yet humble readiness to revise scientific doctrines when the evidence is supportive. We conclude that high variability of response to non-invasive brain stimulation plasticity protocols significantly clouds the interpretation of historical findings in dystonia research. There is an opportunity to wipe the slate clean of assumptions and armed with an informative literature in health, re-evaluate whether excessive plasticity has a causal role in the pathophysiology of dystonia

Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>

Energy buffering systems are key for homeostasis during variations in energy supply. Spiders are the most important predators for insects and therefore key in terrestrial ecosystems. From biomedical interest, spiders are important for their venoms and as a source of potent allergens, such as arginine kinase (AK, EC 2.7.3.3). AK is an enzyme crucial for energy metabolism, keeping the pool of phosphagens in invertebrates, and also an allergen for humans. In this work, we studied AK from the Argentininan spider Polybetes pythagoricus (PpAK), from its complementary DNA to the crystal structure. The PpAK cDNA from muscle was cloned, and it is comprised of 1068 nucleotides that encode a 384-amino acids protein, similar to other invertebrate AKs. The apparent Michaelis-Menten kinetic constant (Km) was 1.7 mM with a kcat of 75 s-1. Two crystal structures are presented, the apoPvAK and PpAK bound to arginine, both in the open conformation with the active site lid (residues 310-320) completely disordered. The guanidino group binding site in the apo structure appears to be organized to accept the arginine substrate. Finally, these results contribute to knowledge of mechanistic details of the function of arginine kinase.Instituto de Investigaciones Bioquímicas de La Plat