Data on ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications

Characterization data of fluorescent nanoparticles made of cellulose acetate (CA-dots) are shown. The data in this article accompanies the research article “Ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications” [1]. The measurements and calculation of brightness of individual CA-dots are presented. The description of conjugation procedure Pluronic F127-Folic Acid copolymer and folic acid is shown. Identification of composition of CA dots using Raman and absorbance spectroscopy is demonstrated. The methods for image analysis of efficiency of CA-dot targeting of epithelial tumors xenografted in zebrafish is presented

Ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications

Cellulose acetate (CA), viscose, or artificial silk are biocompatible human-benign derivatives of cellulose, one of the most abundant biopolymers on earth. While various optical materials have been developed from CA, optical CA nanomaterials are nonexistent. Here we report on the assembly of a new family of extremely bright fluorescent CA nanoparticles (CA-dots), which are fully suitable for in vivo imaging/targeting applications. CA-dots can encapsulate a variety of molecular fluorophores. Using various commercially available fluorophores, we demonstrate that the fluorescence of CA-dots can be tuned within the entire UV–VIS-NIR spectrum. We also demonstrate excellent specific targeting of tumors in vivo, when injected in zebrafish (xenograft model of human cervical epithelial cancer), and unusually strong exvivo topical labeling of colon cancer in mice utilizing CA folate-functionalized nanoparticles.National Science Foundation (U.S.) (Grant CBET 1745530

A quality improvement project to reduce overutilization of blood tests in a teaching hospital

Objective: In hospitals, physicians-in-training are major contributors to the burden of tests ordered, increasing cost and resource utilization. We implemented an intervention to discourage overutilization of the complete blood count (CBC) and the basic metabolic panel (BMP). Methods: An intervention was designed, comprising education on high-value care and burden of over-testing, encouragement of competition, and use of positive reinforcement. The intervention was monitored by a test index determined by dividing the total number of a specific laboratory test ordered for a patient by the total number of hospital days. Results: Following a 6-month intervention, the mean CBC index decreased from 1.56 ± 0.02 to 1.45 ± 0.03 (p < 0.001), and the BMP index, from 1.35 ± 0.02 to 1.14 ± 0.03 (p < 0.001). There was significant interaction between the intervention and the slope of the BMP index trend (p = 0.03), but not the CBC index trend. The intervention had no impact on hospital length of stay and mortality. Conclusion: This quality improvement intervention is an effective approach to reducing overutilization of laboratory tests

The effect of geometric isomerism on the anticancer activity of the monofunctional platinum complex trans-[Pt(NH₃)₂(phenanthridine)Cl]NO₃

A trans-DDP based monofunctional phenanthridine Pt(ii) complex was synthesized and characterized. Its anticancer activity was studied in vitro on a panel of human cancer cell lines and mouse intestinal cancer organoids. This complex displays significant antitumor properties, with a different spectrum of activity than that of classic bifunctional cross-linking agents like cisplatin.National Institutes of Health (U.S.) (Grant CA034992)National Institutes of Health (U.S.) (Grant AG045144)National Institutes of Health (U.S.) (Grant CA034992)National Institutes of Health (U.S.) (Grant CA211184

EXPLORING THE RACIAL DISPARITIES OF COLON CANCER OUTCOMES IN THE SETTING OF OBESITY

Background: African Americans (AA) have higher incidence and mortality from colon cancer compared to Caucasian Americans (CA). The mechanisms that drive this difference are not well understood. Genetic, environmental and socioeconomic factors play important roles in colon cancer pathogenesis and each may contribute to this disparity. One of the important factors that may influence different aspects of colon cancer is obesity. Obesity as a lowgrade inflammatory state creates changes in the epigenetic and metabolic landscape in ways that promote the initiation and progression of colon cancer. In this study, we use a multipronged approach in exploring potential contributory elements that may explain the disproportionate burden of colon cancer in AA patients. Methods: IRB-approved prospective collection of samples from colon cancer patients undergoing surgical resection from the two cohorts (AA/CA, Normal/Obese) were assembled and clinically annotated. The samples included tumor and adjacent non-tumor tissues and blood for plasma isolation (AA=30, CA=23). Tumor tissue DNA was isolated and mutation analysis was acquired via PanelSeq, which included 60 commonly mutated genes in colon cancer. Plasma samples will be sent for metabolomic analysis. The tumor tissue samples were used for stem cell isolation and were cultured as 3-dimensional organoid cultures. Finally, tumor organoids will be transplanted into NOD scid gamma mice using colonoscopy-assisted orthotopic xenotransplantation method that we developed. Results: DNA mutation analysis showed widely different mutational landscape across the samples (Figure 1). Notable mutations included common colon cancer drivers such as APC and KRAS, as well as mismatch repair mutations such as MLH1 and PMS1. AA and CA colon tumor organoids exhibited no significant differences in proliferation (Figure 2). Metabolomics analysis and orthotopic transplantation to be performed. Conclusions: Colon cancer is the third leading cause of cancer death in the U.S. The incidence and mortality are worse in AA patients compared to CA patients. Moreover, obesity is a major modifiable risk factor that plays an important role in the pathogenesis of colon cancer. Here we show that the mutational landscape of colon cancer is distinct between the two cohorts (AA vs. CA) and BMIs (Normal vs. Obese). The metabolomics data is pending but we expect to see differences. Finally, we developed in vitro colon tumor organoid cultures derived from AA patient samples that can be utilized for mechanistic experiments. Tumor organoids will also be used for orthotopic xenotransplantation in our model for colon cancer. To our knowledge, this has never been done with samples from AA patients and opens the door for future work in exploring the pathogenesis of the heightened colon cancer incidence and mortality in this underrepresented population

CCDC 1582154: Experimental Crystal Structure Determination

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures

Data on ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications

Characterization data of fluorescent nanoparticles made of cellulose acetate (CA-dots) are shown. The data in this article accompanies the research article “Ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications” [1]. The measurements and calculation of brightness of individual CA-dots are presented. The description of conjugation procedure Pluronic F127-Folic Acid copolymer and folic acid is shown. Identification of composition of CA dots using Raman and absorbance spectroscopy is demonstrated. The methods for image analysis of efficiency of CA-dot targeting of epithelial tumors xenografted in zebrafish is presented