Development of Quaternized Chitosan Integrated with Nanofibrous Polyacrylonitrile Mat as an Anion-Exchange Membrane

A two-phase anion-exchange membrane was prepared from quaternized chitosan (QCS) integrated with an electrospun polyacrylonitrile (PAN) scaffold by spin coating. To synthesize QCS, glycidyltrimethylammonium chloride in various amounts was introduced into the structure of CS. The characterization of the cast cross-linked QCS (CQCS) membranes by impedance spectroscopy revealed the ionic conductivity (IC) in the range of 2.8 × 10–4 to 8.2 × 10–4 S cm–1 and the degree of quaternization (DQ) of 26.4–51.0%, where the CQCS film with the DQ of 51.0% showed excellent performance. When CQCS was reinforced with a PAN fiber mat, the newly developed composite membrane demonstrated the highest IC of 34 × 10–4 S cm–1 at 80 °C, low swelling, and an almost eightfold increase in tensile strength at a fully hydrated state compared to pristine materials. Moreover, the CQCS/PAN membrane was chemically stable and revealed increasing hydroxide transport during 1 month immersion in alkaline media

Polymer–Metal Complexes in Polyelectrolyte Multilayer Films as Catalysts for Oxidation of Toluene

We report on the binding of metal ions (Me²⁺; Co²⁺ and Cu²⁺) with weak polyelectrolyte multilayers (PEMs), as well as on catalytic activity of PEM–Me²⁺ films for oxidation of toluene. Using several types of PEM films constructed using branched polyethyleneimine (BPEI) or quaterinized poly-4-vinylpyridines (QPVPs) as polycations and poly­(acrylic acid) (PAA) or poly­(styrene sulfonate) (PSS) as polyanions, we found that binding of Co²⁺ and Cu²⁺ ions with a PEM matrix can occur both through coordination to polycationic amino groups and/or ionic binding to polyacid groups. The amount of metal ions loaded within the film increased linearly with film thickness and was strongly dependent on polyelectrolyte type, film assembly pH, and fraction of permanent charge in polymer chains. Among various PEM–Me²⁺ systems, BPEI/PAA–Co²⁺ films assembled at pH 8.5 show the best catalytic performance, probably because of the preservation of high mobility of Co²⁺ ions coordinated to amino groups of BPEI in these films. With BPEI/PAA–Co²⁺ films, we demonstrated that films were highly permeable to reagents and reaction products within hundreds of nanometers of the film bulk; i.e., film catalytic activity increased linearly with layer number up to 30 bilayers and slowed for thicker films

Noneluting Enzymatic Antibiofilm Coatings

We developed a highly efficient, biocompatible surface coating that disperses bacterial biofilms through enzymatic cleavage of the extracellular biofilm matrix. The coating was fabricated by binding the naturally existing enzyme dispersin B (DspB) to surface-attached polymer matrices constructed via a layer-by-layer (LbL) deposition technique. LbL matrices were assembled through electrostatic interactions of poly­(allylamine hydrochloride) (PAH) and poly­(methacrylic acid) (PMAA), followed by chemical cross-linking with glutaraldehyde and pH-triggered removal of PMAA, producing a stable PAH hydrogel matrix used for DspB loading. The amount of DspB loaded increased linearly with the number of PAH layers in surface hydrogels. DspB was retained within these coatings in the pH range from 4 to 7.5. DspB-loaded coatings inhibited biofilm formation by two clinical strains of <i>Staphylococcus epidermidis</i>. Biofilm inhibition was ≥98% compared to mock-loaded coatings as determined by CFU enumeration. In addition, DspB-loaded coatings did not inhibit attachment or growth of cultured human osteoblast cells. We suggest that the use of DspB-loaded multilayer coatings presents a promising method for creating biocompatible surfaces with high antibiofilm efficiency, especially when combined with conventional antimicrobial treatment of dispersed bacteria