764 research outputs found
Isolation of active genes containing CAG repeats by DNA strand invasion by a peptide nucleic acid.
Pole dynamics for the Flierl-Petviashvili equation and zonal flow
We use a systematic method which allows us to identify a class of exact
solutions of the Flierl-Petvishvili equation. The solutions are periodic and
have one dimensional geometry. We examine the physical properties and find that
these structures can have a significant effect on the zonal flow generation.Comment: Latex 40 pages, seven figures eps included. Effect of variation of
g_3 is studied. New references adde
Patient participation in general practice-based undergraduate teaching: a focus group study of patient perspectives
Background: Patients make a crucial contribution to undergraduate medical education. While a national
resource is available for patients participating in research, none is at yet available for
education. / Aim: This study aimed to explore what information patients would like about participation in
general practice-based undergraduate medical education and how they would like to obtain
this information. / Design and Setting: We conducted two focus groups in London-based practices involved in both undergraduate
and postgraduate teaching. / Method: Patients were recruited both with and without teaching experience using leaflets, posters and
patient participation groups. An open-ended topic guide explored three areas: perceived
barriers which participants anticipated, or had experienced; patient roles in medical
education; and what help would support participation. Focus groups were audio-taped,
transcribed and analysed thematically. / Results: Patients suggested ways of professionalising the teaching process, making information
available to patients about confidentiality; iterative consent; and normalising teaching in the
practice. Patients highlighted the importance of relationships: making information available
about their GP’s involvement, and initiating student-patient interactions. Participants
emphasised educational principles to maximise exchange of information including active
participation of students; patient identification of student learner needs; and exchange of
feedback. / Conclusion: This study will inform development of patient information resources to support their
participation in teaching and access to information both before and during general practicebased
teaching encounters
Mapping Dynamic Histone Acetylation Patterns to Gene Expression in Nanog-depleted Murine Embryonic Stem Cells
Embryonic stem cells (ESC) have the potential to self-renew indefinitely and
to differentiate into any of the three germ layers. The molecular mechanisms
for self-renewal, maintenance of pluripotency and lineage specification are
poorly understood, but recent results point to a key role for epigenetic
mechanisms. In this study, we focus on quantifying the impact of histone 3
acetylation (H3K9,14ac) on gene expression in murine embryonic stem cells. We
analyze genome-wide histone acetylation patterns and gene expression profiles
measured over the first five days of cell differentiation triggered by
silencing Nanog, a key transcription factor in ESC regulation. We explore the
temporal and spatial dynamics of histone acetylation data and its correlation
with gene expression using supervised and unsupervised statistical models. On a
genome-wide scale, changes in acetylation are significantly correlated to
changes in mRNA expression and, surprisingly, this coherence increases over
time. We quantify the predictive power of histone acetylation for gene
expression changes in a balanced cross-validation procedure. In an in-depth
study we focus on genes central to the regulatory network of Mouse ESC,
including those identified in a recent genome-wide RNAi screen and in the
PluriNet, a computationally derived stem cell signature. We find that compared
to the rest of the genome, ESC-specific genes show significantly more
acetylation signal and a much stronger decrease in acetylation over time, which
is often not reflected in an concordant expression change. These results shed
light on the complexity of the relationship between histone acetylation and
gene expression and are a step forward to dissect the multilayer regulatory
mechanisms that determine stem cell fate.Comment: accepted at PLoS Computational Biolog
An NLO QCD analysis of inclusive cross-section and jet-production data from the ZEUS experiment
The ZEUS inclusive differential cross-section data from HERA, for charged and
neutral current processes taken with e+ and e- beams, together with
differential cross-section data on inclusive jet production in e+ p scattering
and dijet production in \gamma p scattering, have been used in a new NLO QCD
analysis to extract the parton distribution functions of the proton. The input
of jet data constrains the gluon and allows an accurate extraction of
\alpha_s(M_Z) at NLO;
\alpha_s(M_Z) = 0.1183 \pm 0.0028(exp.) \pm 0.0008(model)
An additional uncertainty from the choice of scales is estimated as \pm
0.005. This is the first extraction of \alpha_s(M_Z) from HERA data alone.Comment: 37 pages, 14 figures, to be submitted to EPJC. PDFs available at
http://durpdg.dur.ac.uk/hepdata in LHAPDFv
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