899 research outputs found
PI3K signalling in leptin receptor cells: Role in growth and reproduction
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149245/1/jne12685_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149245/2/jne12685.pd
A Study of Sexual Behavior, Sex Information and Self-Concept in Adolescent Venereal Disease Patients
A Research Report Presented to the Faculty of the Raymond A. Kent School of Social Work, University of Louisville, in partial fulfillment of the requirements for the degree of Master of Science in Social Work by Joan Allen, Ralph H. Childers, Hubert Crawford, Olin Gentry, Jr., Ralph Hollon, Carol Hill and Evelyn J. McKissick in June of 1965
A study of adverse reaction algorithms in a drug surveillance program
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110093/1/cptclpt1985156.pd
ExCEEd teaching workshop: Tenth year anniversary
In response to the need for faculty training, the American Society of Civil Engineers developed and funded the ExCEEd (Excellence in Civil Engineering Education) Teaching Workshop that is today – the summer of 2008 – celebrating its tenth year of existence. For the past decade, nineteen ExCEEd Teaching Workshops (ETW) have been held at the United States Military Academy, the University of Arkansas, and Northern Arizona University, with two more workshops scheduled for this summer for a total of 21 offerings. ETW has realized 449 graduates from 203 different U.S. and international colleges and universities. This paper summarizes the content of ETW, assesses its effectiveness, highlights changes in the program as a result of the assessment, and outlines future directions. The assessment data were obtained from multiple survey instruments conducted during each workshop, surveys taken six months to a year after the workshop, and a ten year longitudinal survey
The JAX Synteny Browser for mouse-human comparative genomics.
Visualizing regions of conserved synteny between two genomes is supported by numerous software applications. However, none of the current applications allow researchers to select genome features to display or highlight in blocks of synteny based on the annotated biological properties of the features (e.g., type, function, and/or phenotype association). To address this usability gap, we developed an interactive web-based conserved synteny browser, The Jackson Laboratory (JAX) Synteny Browser. The browser allows researchers to highlight or selectively display genome features in the reference and/or the comparison genome according to the biological attributes of the features. Although the current implementation for the browser is limited to the reference genomes for the laboratory mouse and human, the software platform is intentionally genome agnostic. The JAX Synteny Browser software can be deployed for any two genomes where genome coordinates for syntenic blocks are defined and for which biological attributes of the features in one or both genomes are available in widely used standard bioinformatics file formats. The JAX Synteny Browser is available at: http://syntenybrowser.jax.org/. The code base is available from GitHub: https://github.com/TheJacksonLaboratory/syntenybrowser and is distributed under the Creative Commons Attribution license (CC BY)
Quantitative Analysis of the DNA Methylation Sensitivity of Transcription Factor Complexes
Although DNA modifications play an important role in gene regulation, the underlying mechanisms remain elusive. We developed EpiSELEX-seq to probe the sensitivity of transcription factor binding to DNA modification in vitro using massively parallel sequencing. Feature-based modeling quantifies the effect of cytosine methylation (5mC) on binding free energy in a position-specific manner. Application to the human bZIP proteins ATF4 and C/EBPβ and three different Pbx-Hox complexes shows that 5mCpG can both increase and decrease affinity, depending on where the modification occurs within the protein-DNA interface. The TF paralogs tested vary in their methylation sensitivity, for which we provide a structural rationale. We show that 5mCpG can also enhance in vitro p53 binding and provide evidence for increased in vivo p53 occupancy at methylated binding sites, correlating with primed enhancer histone marks. Our results establish a powerful strategy for dissecting the epigenomic modulation of protein-DNA interactions and their role in gene regulation
Pulmonary Effects of Indoor- and Outdoor-Generated Particles in Children with Asthma
Most particulate matter (PM) health effects studies use outdoor (ambient) PM as a surrogate for personal exposure. However, people spend most of their time indoors exposed to a combination of indoor-generated particles and ambient particles that have infiltrated. Thus, it is important to investigate the differential health effects of indoor- and ambient-generated particles. We combined our recently adapted recursive model and a predictive model for estimating infiltration efficiency to separate personal exposure (E) to PM(2.5) (PM with aerodynamic diameter ≤2.5 μm) into its indoor-generated (E(ig)) and ambient-generated (E(ag)) components for 19 children with asthma. We then compared E(ig) and E(ag) to changes in exhaled nitric oxide (eNO), a marker of airway inflammation. Based on the recursive model with a sample size of eight children, E(ag) was marginally associated with increases in eNO [5.6 ppb per 10-μg/m(3) increase in PM(2.5); 95% confidence interval (CI), −0.6 to 11.9; p = 0.08]. E(ig) was not associated with eNO (−0.19 ppb change per 10μg/m(3)). Our predictive model allowed us to estimate E(ag) and E(ig) for all 19 children. For those combined estimates, only E(ag) was significantly associated with an increase in eNO (E(ag): 5.0 ppb per 10-μg/m(3) increase in PM(2.5;) 95% CI, 0.3 to 9.7; p = 0.04; E(ig): 3.3 ppb per 10-μg/m(3) increase in PM(2.5); 95% CI, −1.1 to 7.7; p = 0.15). Effects were seen only in children who were not using corticosteroid therapy. We conclude that the ambient-generated component of PM(2.5) exposure is consistently associated with increases in eNO and the indoor-generated component is less strongly associated with eNO
CODE-2 : moored array and large-scale data report
The Coastal Ocean Dynamics Experiment
(CODE) was undertaken to identify and study
the important dynamical processes which
govern the wind-driven motion of coastal
water over the continental shelf. The
initial effort in this multi-year, multi-institutional
research program was to obtain
high-quality data sets of all the
relevant physical variables needed to construct
accurate kinematic and dynamic descriptions
of the response of shelf water
to strong wind forcing in the 2 to 10 day
band. A series of two small-scale, densely-
instrumented field experiments of approximately
four months duration (called CODE-1
and CODE-2) were designed to explore and
to determine the kinematics and momentum
and heat balances of the local wind-driven
flow over a region of the northern California
shelf which is characterized by both
relatively simple bottom topography and
large wind stress events in both winter
and summer. A more lightly instrumented,
long -term, large-scale component was
designed to help separate the local wind-driven
response in the region of the small-scale
experiments from motions generated
either offshore by the California Current
system or in some distant region along the
coast, and also to help determine the seasonal
cycles of the atmospheric forcing,
water structure, and coastal currents over
the northern California shelf.
The first small-scale experiment
(CODE-1) was conducted between April and
August, 1981 as a pilot study in "which
primary emphasis was placed on characterizing
the wind-driven "signal" and the
"noise" from which this signal must be
extracted. In particular, CODE-1 was
designed to identify the key features of
the circulation and its variability over
the northern California shelf and to
determine the important time and length
scales of the wind-driven response. The
second small-scale experiment (CODE-2) was
conducted between April and August, 1982
and was designed to sample more carefully
the mesoscale horizonta1 variability
observed in CODE-1. This report presents a
basic description of the moored array data
and some other Eulerian data collected
during CODE-2. A brief description of the
CODE-2 field program is presented first,
followed by a description of the common
data analysis procedures used to produce
the various data sets presented here. Then
basic descriptions of the following data
sets are presented: (a) the coastal and
moored meteorological measurements, (b)
the moored current measurements, (c) array
plots of the surface wind stress and near-surface
current measurements, (d) the
moored temperature and conductivity observations,
(e) the bottom pressure measurements,
and (f) the wind and adjusted
coastal sea level observations obtained as
part of the CODE-2 large-scale component.This work has
been supported by the National Science
Foundation
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