Distribution of VHSV positive fish in the Great Lakes from 2003 through 2008 as reported by the U.S. Department of Agriculture Animal and Plant Health Inspection Service [12] and the distribution of documented invasion hotspots [18], [27].

<p>Distribution of VHSV positive fish in the Great Lakes from 2003 through 2008 as reported by the U.S. Department of Agriculture Animal and Plant Health Inspection Service <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010156#pone.0010156-Center1" target="_blank">[12]</a> and the distribution of documented invasion hotspots <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010156#pone.0010156-Grigorovich1" target="_blank">[18]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010156#pone.0010156-Cangelosi1" target="_blank">[27]</a>.</p

Fish analyzed for VHSV and number of fish determined to be positive with data on fish sizes and known vulnerability to VHSV.

<p>1. VHSV susceptibility is reported by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010156#pone.0010156-Center1" target="_blank">[12]</a> although other species can be infected.</p

Detection of VHSV in fish and water and the presence of VHSV positive fish at sampling sites in and outside of invasion hotspots shown in Figure 1.

<p>Detection of VHSV in fish and water and the presence of VHSV positive fish at sampling sites in and outside of invasion hotspots shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010156#pone-0010156-g001" target="_blank">Figure 1</a>.</p

VHSV detection in fish and water using qRT-PCR and cell culture assays shown by site, type of site, and number of fish tested.

<p>VHSV detection in fish and water using qRT-PCR and cell culture assays shown by site, type of site, and number of fish tested.</p

Distribution of VHSV positive fish and water at sites classified as commercial shipping harbors, recreational boating centers, and open shoreline.

<p>Distribution of VHSV positive fish and water at sites classified as commercial shipping harbors, recreational boating centers, and open shoreline.</p