Interleukine-1 et arthrite juvénile idiopathique systémique

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Validation of Twelve Biomarkers of Response to IFN-γ.

<p>The expression of 12 genes was validated by qRT-PCR. The Wilcoxon signed rank test was used to test for statistical significance (*<i>p</i><0.05 Non stimulated vs LPS-unprimed cells; § <i>p</i><0.05 LPS-unprimed cells vs LPS-primed cells; ¤<i>p</i><0.05 LPS-primed cells vs LPS-primed cells+ IFN-γ).</p

IFN-γ treatment restores the expression of candidates biomarkers in whole blood from septic patients.

<p>Whole blood from seven healthy donors (open columns) or septic patients (black columns) was stimulated with LPS (100 ng/ml) ± rIFN-γ1b (100 ng/ml) overnight and the expression of the biomarkers previously identified was measured by qRT-PCR. Data are presented as median ± IQR. Comparison between healthy volunteers and septic patients treated with LPS was performed using the Mann-Whitney U test (* <i>P</i><0.05) whereas evaluation of rIFN-y effect was performed using the Wilcoxon signed rank test (§ <i>P</i><0.05).</p

Identification of Biomarkers of Response to IFN-γ.

<p>(A). PBMCs from 6 HD were stimulated according to the scheme shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068218#pone-0068218-g001" target="_blank">Figure 1</a>. After the stimulation, RNA was extracted and hybridized to Affymetrix U133 Plus chips. 70 transcripts were found differentially expressed between the « non-stimulated » condition and the « LPS unprimed » condition and 43 between the « LPS unprimed » and « LPS primed » conditions (paired t-test p<0.05, av. FC>2 in at least 4 donors). The ratios “LPS primed”/”LPS unprimed” of the top 20 non tolerizable genes and the top 20 tolerizable genes are represented. (C). Out of the 113 transcripts that were found dysregulated in tolerant monocytes, the expression of 47 transcripts was significantly restablished upon adding rIFN-γ. Those 47 transcripts were arranged by hierarchical clustering to reveal differential expression. Expression values are normalized per gene to the unstimulated condition. Transformed expression levels are indicated by color scale, with red representing relative high expression and blue indicating relative low expression. A list of the genes shown in this figure is available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068218#pone.0068218.s001" target="_blank">Table S1</a>.</p

The Endotoxin Tolerance Model.

<p>(A). Schematic representation of the endotoxin tolerance model used in the study. (B) The expression of TNF-a and IL-10 was measured by qRT-PCR upon treatment of the cells according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068218#pone-0068218-g001" target="_blank">Figure 1A</a>. The Wilcoxon signed rank test was used to test for statistical significance (*<i>p</i><0.05 Non stimulated vs LPS-unprimed cells; § <i>p</i><0.05 LPS-unprimed cells vs LPS-primed cells).</p

Patient’s Characteristics.

<p>Results are presented as median and interquartile range (Q1–Q3) for continuous variables and as number of cases for categorical variables.</p><p>SAPSII, Simplified Acute Physiology Score; SOFA, Sequential Organ Failure Assessment;</p>*<p>The most common comorbidity observed was myocardial infarcts (2/7 patients) and Moderate or severe liver disease (2/7 patients).</p

Clinical and laboratory characteristics of symptomatic healthcare workers with suspected COVID-19: a prospective cohort study

A comprehensive clinical and microbiological assessments of COVID-19 in front-line healthcare workers (HCWs) is needed. Between April 10th and May 28th, 2020, 319 HCWs with acute illness were reviewed. In addition to SARS-CoV-2 RT-PCR screening, a multiplex molecular panel was used for testing other respiratory pathogens. For SARS-CoV-2 positive HCWs, the normalized viral load, viral culture, and virus neutralization assays were performed weekly. For SARS-CoV-2 negative HCWs, SARS-CoV-2 serological testing was performed one month after inclusion. Among the 319 HCWs included, 67 (21.0%) were tested positive for SARS-CoV-2; 65/67 (97.0%) developed mild form of COVID-19. Other respiratory pathogens were found in 6/66 (9.1%) SARS-CoV-2 positive and 47/241 (19.5%) SARS-Cov-2 negative HCWs (p?=?0.07). The proportion of HCWs with a viral load?&gt;?5.0 log10cp/mL (Ct value??37). More than 90% of cultivable virus had a viral load?&gt;?4.5 log10cp/mL (Ct