DPHL: A DIA Pan-human Protein Mass Spectrometry Library for Robust Biomarker Discovery

To address the increasing need for detecting and validating protein biomarkers in clinical specimens, mass spectrometry (MS)-based targeted proteomic techniques, including the selected reaction monitoring (SRM), parallel reaction monitoring (PRM), and massively parallel data-independent acquisition (DIA), have been developed. For optimal performance, they require the fragment ion spectra of targeted peptides as prior knowledge. In this report, we describe a MS pipeline and spectral resource to support targeted proteomics studies for human tissue samples. To build the spectral resource, we integrated common open-source MS computational tools to assemble a freely accessible computational workflow based on Docker. We then applied the workflow to generate DPHL, a comprehensive DIA pan-human library, from 1096 data-dependent acquisition (DDA) MS raw files for 16 types of cancer samples. This extensive spectral resource was then applied to a proteomic study of 17 prostate cancer (PCa) patients. Thereafter, PRM validation was applied to a larger study of 57 PCa patients and the differential expression of three proteins in prostate tumor was validated. As a second application, the DPHL spectral resource was applied to a study consisting of plasma samples from 19 diffuse large B cell lymphoma (DLBCL) patients and 18 healthy control subjects. Differentially expressed proteins between DLBCL patients and healthy control subjects were detected by DIA-MS and confirmed by PRM. These data demonstrate that the DPHL supports DIA and PRM MS pipelines for robust protein biomarker discovery. DPHL is freely accessible at https://www.iprox.org/page/project.html?id=IPX0001400000

Theoretical study of new configuration of photovoltaic/thermal solar collector (PV/T) design

The combination of solar thermal and PV technologies leads to form a single module called PVT system. This system is able to produce heat and electricity simultaneously. In this paper, a new configuration and design of PV/T system are proposed, and a theoretical study of this system is evaluated. The PV/T consists of certain layers which are the glass, air gap, PV panel, absorber plate and insulating material layer. The monocrystalline PV and spiral flow absorber plate are used in this study. The results of the system performance under typical sunny climatic Malaysian condition are presented. The maximum thermal and electrical efficiencies obtained are 64.4 % and 12.13%, respectively. The effect of mass flow rate on the outlet water temperature is also evaluated. It is shown that the optimum mass flow rate for the designed system is between 0.005 and 0.075 kg/s

A review of underground building towards thermal energy efficiency and sustainable development

Global warming has posed a great challenge to the survival of mankind. The increasing atmospheric concentration of carbon dioxide is widely recognized as the largest contributor of global warming. Hence, world-wide attention towards energy conservation has grown markedly to reduce the carbon dioxide emission. In the context of building energy performance, the ancient wisdom of using the earth as temperature moderator against harsh weather has impressive potential to become a solid solution against the energy inefficiency of Heating, Ventilation, and Air Conditioning system (HVAC) in building. This creative and traditional passive cooling technique has been also made it possible to achieve most criteria of sustainable development concerning the world׳s growing housing demand, climate change, fossil-fuel depletion as well as limited land area and resources. In this paper, underground buildings were reviewed from preliminary aspects such as historical background, classification and subsequent thermal energy performance criteria. It has tried to elaborate the thermal performance variables, and conduct further study of benefits and drawbacks of this passive cooling technique toward different perspectives of sustainable development. With intention to gain wide acceptance in the modern society, this paper has proposed a conceptual design of earth sheltered home, which incorporated different types of the building techniques. Eventually earth sheltered buildings worldwide were summarized in a well illustrative form to highlight the different designs, applications, locations, timelines and climates. In addition, a deductive model is graphically represented for the virtual overview of underground structures that set the foundation to further investigate sustainability of this building envelope technique

Analisis Perbandingan Tingkat Stress Mahasiswa Saintek dan Soshum dalam Pembelajaran Daring pada Masa Pandemi Covid-19 Berbasis Internet Of Things

The spread of COVID-19 has occurred in 2019, which has had an enormous impact on the world's population. The continuous spread of COVID-19 has caused several countries to reduce the transmission of COVID-19, one of which is implementing online learning at schools and universities. The impact that occurs on students due to home study policies makes some students feel anxious and depressed. There are two study groups at the University of Lambung Mangkurat, namely the Social Humanities (Soshum) and Science and Technology (Saintek). The student who majored in science, technology, and social science has a different way of finding the information needed and understanding every material available online. This problem is due to cultural differences in the applied learning system. These differences certainly cause different stress levels for each student majoring in science, technology, and social sciences. Therefore, this study was conducted to determine the difference in stress levels experienced by Lambung Mangkurat University students in science, technology, and social media while online. This study uses two stages to compare the results of student stress levels, including filling out the DASS42 questionnaire and direct testing with 3 IoT sensors, namely GSR, body temperature (GY-906 MLX90614 Infrared Temperature Sensor), and pulse rate (MAX30102 Pulse Oximeter & Heart-Rate Sensor). The application of the Fuzzy Logic method is used as a parameter measurement when measuring IoT-based stress levels

Genomic and Epigenomic Profiling of High-Risk Intestinal Metaplasia Reveals Molecular Determinants of Progression to Gastric Cancer

10.1016/j.ccell.2017.11.018Cancer Cell331137-150 e5CCAE