2 research outputs found
The Use of Minimal RNA Toeholds to Trigger the Activation of Multiple Functionalities
Current work reports the use of single-stranded
RNA toeholds of different lengths to promote the reassociation of
various RNA–DNA hybrids, which results in activation of multiple
split functionalities inside human cells. The process of reassociation
is analyzed and followed with a novel computational multistrand secondary
structure prediction algorithm and various experiments. All of our
previously designed RNA/DNA nanoparticles employed single-stranded
DNA toeholds to initiate reassociation. The use of RNA toeholds is
advantageous because of the simpler design rules, the shorter toeholds,
and the smaller size of the resulting nanoparticles (by up to 120
nucleotides per particle) compared to the same hybrid nanoparticles
with single-stranded DNA toeholds. Moreover, the cotranscriptional
assemblies result in higher yields for hybrid nanoparticles with ssRNA
toeholds