1 research outputs found
In Vitro Fermentation of Xylooligosaccharides Produced from Miscanthus × <i>giganteus</i> by Human Fecal Microbiota
Purified xylooligosaccharides from Miscanthus × giganteus (M×G XOS) were
used in an in vitro fermentation experiment inoculated with human
fecal microbiota. A commercial XOS product and pectin were used as
controls. Decreases in pH by 2.3, 2.4, and 2.0 units and production
of short-chain fatty acids (SCFA; acetic acid, 7764.2, 6664.1, and
6387.9 μmol/g; propionic acid, 1006.7, 1089.5, and 661.5 μmol/g;
and butyric acid, 955.5, 1252.9, and 917.7 μmol/g) were observed
in M×G XOS, commercial XOS, and pectin medium after 12 h of fermentation,
respectively. Titers of Bifidobacterium spp., Lactobacillus spp., and Escherichia coli increased when fed all three substrates
as monitored by qPCR. There was no significant trend for Clostridium perfringens. During fermentation, M×G
XOS was statistically equivalent in performance to the commercial
XOS sample as measured by culture acidification and growth of health-promoting
bacteria and resulted in the highest SCFA production among the three
substrates