36 research outputs found

    Stereo Photoactivated Localization Microscopy for Super-Resolution 3D Bioimaging

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    Superpositions of the Orbital Angular Momentum for Applications in Quantum Experiments

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    Two different experimental techniques for preparation and analyzing superpositions of the Gaussian and Laguerre-Gassian modes are presented. This is done exploiting an interferometric method on the one hand and using computer generated holograms on the other hand. It is shown that by shifting the hologram with respect to an incoming Gaussian beam different superpositions of the Gaussian and the Laguerre-Gaussian beam can be produced. An analytical expression between the relative phase and the amplitudes of the modes and the displacement of the hologram is given. The application of such orbital angular momenta superpositions in quantum experiments such as quantum cryptography is discussed.Comment: 18 pages, 4 figures. to appear in Journal of Optics

    Visualizing KcsA Conformational Changes upon Ion Binding by Infrared Spectroscopy and Atomistic Modeling

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    The effect of ion binding in the selectivity filter of the potassium channel KcsA is investigated by combining amide I Fourier-transform infrared spectroscopy with structure-based spectral modeling. Experimental difference IR spectra between K[superscript +]-bound KcsA and Na[superscript +]-bound KcsA are in good qualitative agreement with spectra modeled from structural ensembles generated from molecular dynamics simulations. The molecular origins of the vibrational modes contributing to differences in these spectra are determined not only from structural differences in the selectivity filter but also from the pore helices surrounding this region. Furthermore, the coordination of K[superscript +] or Na[superscript +] to carbonyls in the selectivity filter effectively decouples the vibrations of those carbonyls from the rest of the protein, creating local probes of the electrostatic environment. The results suggest that it is necessary to include the influence of the surrounding helices in discussing selectivity and transport in KcsA and, on a more general level, that IR spectroscopy offers a nonperturbative route to studying the structure and dynamics of ion channels.Vienna Science and Technology Fund (Project VRG10-11)University of Vienna (Research Platform Quantum Phenomena and Nanoscale Biological System

    Direct detection of a single photon by humans

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    Despite investigations for over 70 years, the absolute limits of human vision have remained unclear. Rod cells respond to individual photons, yet whether a single-photon incident on the eye can be perceived by a human subject has remained a fundamental open question. Here we report that humans can detect a single-photon incident on the cornea with a probability significantly above chance. This was achieved by implementing a combination of a psychophysics procedure with a quantum light source that can generate single-photon states of light. We further discover that the probability of reporting a single photon is modulated by the presence of an earlier photon, suggesting a priming process that temporarily enhances the effective gain of the visual system on the timescale of seconds

    Experimental Quantum Cryptography with Qutrits

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    We produce two identical keys using, for the first time, entangled trinary quantum systems (qutrits) for quantum key distribution. The advantage of qutrits over the normally used binary quantum systems is an increased coding density and a higher security margin. The qutrits are encoded into the orbital angular momentum of photons, namely Laguerre-Gaussian modes with azimuthal index l +1, 0 and -1, respectively. The orbital angular momentum is controlled with phase holograms. In an Ekert-type protocol the violation of a three-dimensional Bell inequality verifies the security of the generated keys. A key is obtained with a qutrit error rate of approximately 10 %.Comment: New version includes additional references and a few minor changes to the manuscrip
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