Human Pappilomavirus Genotype in Cervical Tissue of Patients with Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, and CIN 3

Objectives: to determine the genotype of HPV in patients with precancerous lesions of cervical tissue.Materials and Methods: An observational study with cross sectional study of patients paraffin block CIN1, CIN2, CIN3 was conducted in Dr Soetomo Hospital. HPV DNA was extracted from paraffin blocks, then performed PCR and genotyping of HPV. The sample consisted of 28 patients with cervical tissue paraffin blocks CIN1, CIN2 and CIN3. Patients aged between 26-74 years (standard deviation 10,12).Results: HPV genotypes that infect patients with CIN1 were HPV16 and 18, CIN2 were HPV16 and 52 and CIN3 were HPV16, 67, and combined infection HPV16/67 and HPV52/67. HPV genotypes in a single infection were 26/28 (HPV16, HPV18, HPV52 and HPV67), and multiple infections were 2/28 (HPV16/67 and HPV52/67).Conclusion: The most dominant HPV genotypes infect patients with precancerous lesions of the cervix were HPV16, HPV67, HPV52, and HPV18

Pengaruh Jenis Kemasan terhadap Kondisi Penyimpanan Gabah Kering Panen, Rendemen Giling dan Beras Kepala

Aging is a natural and spontaneous phenomenon which occurs during storage of dry grain, involving physical changes and chemical that would alter the quality of the cooking, processing, taste and nutritional value as well as affect the commercial value of rice. Naturally aging takes a long time between 6-12 months so it is less economical. Aging may be accelerated by increasing the grain moisture content and storage temperature. Freshly harvested rough rice contains high moisture content ranging between 21-26% and respiration activity is still high which generate heat that can be accumulated by the packaging, so the grain temperature increases. The purpose of this study was to determine the types of packaging which can increase the temperature and RH storage, milling yield and head rice yield. Grain varieties used are Siam Pandak with five types of packaging, ie black plastic bags, sacks, cans, wood and tarpaulin, and stored for 72 hours. As a control, direct drying grain harvest is used. Observations are including temperature and RH in packaging, milling recovery and head rice yields. The results showed that all types of packaging used can increase the temperature and RH in the packaging than the temperature and RH in storage space. The most high milling recovery obtained from black plastic packaging, while the head rice yields all kinds of packaging meets the quality criteria V ISO 6128-2008 except wood packaging, although based on the percentage of head rice, tarpaulins packaging has the highest head rice

Skrining Kanker Serviks Dengan Pemeriksaan Pap Smear Di Puskesmas Tanah Kali Kedinding Surabaya Dan Rumah Sakit Mawadah Mojokerto

Tujuan: Penelitian ini bertujuan untuk mengetahui prevalensi lesi prekanker serviks dengan cara melakukan skrining kanker serviks menggunakan pemeriksaan Pap smear.Bahan dan Metode: Penelitian ini merupakan penelitian observasional deskriptif dengan metode pendekatan cross sectional yang dilakukan pada 140 wanita, terdiri dari 90 orang dari Puskes-mas Tanah Kali Kedinding Surabaya, dan 50 orang dari Rumah Sakit Mawadah Mojokerto, dengan usia 20-70 tahun. Pemeriksaan spesimen sitologi serviks dengan pengecatan Papaniculaou dan klasifikasi hasil pemeriksaaan sitologi berdasar-kan klasifikasi Papaniculaou dan sistim Bethesda. Pemeriksaan inspeksi visual asam asetat (IVA) adalah metode lain untuk skrining kanker serviks dilakukan dengan mengoleskan asam asetat 5% pada area serviks dan melakukan pengamatan satu menit kemudian.Hasil: Hasil pemeriksaaan Pap smear menunjukkan Papaniculaou kelas I (sama dengan normal pada klasifikasi sistim Bethesda) yaitu 12.1%, kelas II (sama dengan NILM pada klasifikasi sistim Bethesda) yaitu 86, 4%, dan kelas III (sama dengan LSIL pada klasifikasi sistim Bethesda) yaitu 1,4%. Hasil pemeriksaan IVA menunjukkan 6,43% positif dan 93,57% negatif. Hasil pemeriksa-an IVA positif terdapat pada 9/140 orang yang merupakan proses keradangan dan infeksi, bukan merupakan lesi prekanker.Simpulan: Prevalensi lesi prekanker yaitu 1,4%. Pemeriksaan IVA menunjukkan hasil positif semu yang disebabkan oleh proses radang atau infeksi pada serviks

Pengaruh Formulasi Ukuran Partikel Dan Suhu Pengeringan Terhadap Aktivitas Antioksidan Teh Herbal Celup Bawang Dayak (Eleutherine Palmifolia (L.) Merr)

Bawang Dayak (Eleutherine palmifolia (L.) Merr.) is known as one of antioxidants sources because it has natural bioactive compounds such as phenol, flavonoids, tannins, glycosides, steroids, alkaloids which have good health benefits. Antioxidant can inhibit and prevent the damage caused by oxidation process of free radicals. Because of these benefits, bawang dayak is potential to be processed to a product such as herbal tea. The study aims to determine the effect of particle size formulation on drying temperature variation on antioxidant activity of bawang dayak herbal tea. The research method used is a randomized block design (RAK) of drying temperature consisting of one grouped factor that is the size formulation factor (F). The analysis performed on tea stock were water content, ash content, and presence of phytochemical compounds, while in brewed tea were acidity (pH), antioxidant activity (DPPH method), and sensory analysis (hedonic scale 1 – 5). The results indicated that the particle size formulation on drying temperature variation had an effect on the antioxidant activity. The best result was obtained from 100% coarse size formulation at 50oC drying temperature with the value of inhibition percentage (%) at 10 mg/ml of brewed tea is 77.38% and IC50 is 4.57 mg /ml brewed tea. The results of sensory analysis with hedonic scale for the treatment are for aroma (3.00; like slightly) and color (3.56; like), while taste can still be improved (2.32; dislike)