Apoptosis detection.

<p>PC3 cells were grown on cell culture slides and treated with EGCG and EGCG-LDH and void LDH for 48 h. Apoptosis was determined, as detailed in Materials and Methods. Representative photomicrographs from each treatment group showing induction of apoptosis (green fluorescence). Data are from experiment repeated thrice with similar results.</p

Colony formation.

<p>PC3 cells were treated with EGCG and EGCG-LDH with different concentrations, and the plates were observed for colonies, counted, and plotted as a bar graph. Bars, SE. *, P < 0.05; **, P < 0.01 compared with the vehicle-treated controls. The results are from a representative experiment repeated two times with similar results.</p

a) zeta potential and b) electrophoretic mobility spectra of EGCG-LDH nanohybrids.

<p>a) zeta potential and b) electrophoretic mobility spectra of EGCG-LDH nanohybrids.</p

PC3 cells exhibited early apoptotic (indicated by blue arrows) and necrotic and late apoptotic (indicated by yellow and red arrows, respectively) cell death after 48 h post exposure to 25 μM EGCG-LDH.

<p>PC3 cells exhibited early apoptotic (indicated by blue arrows) and necrotic and late apoptotic (indicated by yellow and red arrows, respectively) cell death after 48 h post exposure to 25 μM EGCG-LDH.</p

Release rate constants and R² coefficients obtained from release data fitting analyses based on kinetic equations.

<p>Release rate constants and R² coefficients obtained from release data fitting analyses based on kinetic equations.</p

X-ray diffraction patterns for LDH-NO₃ particles.

<p>X-ray diffraction patterns for LDH-NO₃ particles.</p

Percent of apoptotic cells.

<p>annexin V- propidium were counted in the field of view, which included 1000 cells, using an optical microscope. (Magnification, ×400).</p

Plots of kinetic equations of (A) first-orderkinetic model, (B) parabolic diffusion model,(C) Bhaskar equation and (D) modified Freundlich model for the release of EGCG from EGCG-LDH nanoparticle at pH of 7.4 and 4.25.

<p>Plots of kinetic equations of (A) first-orderkinetic model, (B) parabolic diffusion model,(C) Bhaskar equation and (D) modified Freundlich model for the release of EGCG from EGCG-LDH nanoparticle at pH of 7.4 and 4.25.</p

In-vitro Release profiles of EGCG from EGCG-LDH nanoparticle and physical mixture; at pH of a) 7.45 and b) 4.25.

<p>In-vitro Release profiles of EGCG from EGCG-LDH nanoparticle and physical mixture; at pH of a) 7.45 and b) 4.25.</p

Fourier transform infrared spectroscopy spectrum of a) LDH-NO3 and b) EGCG-LDH.

<p>Fourier transform infrared spectroscopy spectrum of a) LDH-NO3 and b) EGCG-LDH.</p