Određivanje razine nekih esencijalnih (željezo, bakar, cink) i toksičnih (olovo, kadmij) metala u krvi ovaca te u uzorcima vode, biljaka i tla u sjeverozapadnoj Turskoj.

The aim of this investigation was to determine the levels of trace elements (zinc, iron, copper), as well as of lead and cadmium, in both the soil and in organism in order to obtain more efficient economical results and healthier sheep breeds in Northwest Turkey. Based on the results of these analyses, we plan to make suggestions to veterinarians and sheep breeders in that region on how to improve their performance. The study was carried out on 400 sheep from different sites in Northwest Turkey and included the collection of water (n = 5), plant (n = 5) and soil (n = 5) samples from every district. When serum parameters were examined an important decrease in iron levels was found in the 8th, 9th and 10th districts, while the lowest zinc levels were found in the 1st and 7th districts, the lowest copper levels in the 1st, 3rd, 5th and 6th districts, the highest lead levels in the 9th and 10th districts, and the highest cadmium levels in the 2nd, 5th and 6th districts. Analyses of water, plant and soil samples showed significant differences in zinc and copper levels between districts. High levels of lead were detected in water and plant samples. We are of the opinion that important improvements will be obtained in the treatment and control of diseases due to deficiency of trace elements and of parasitic, bacterial and viral diseases due to related systems affected by such deficiencies if these results are appropriately communicated to animal breeders and veterinary surgeons operating in the regions in question.Cilj istraživanja bio je odrediti razinu elemenata u tragovima (cink, željezo, bakar), te olova i kadmija u tlu i organizmu ovaca kako bi se polučili bolji gospodarski rezultati i uzgajale zdravije pasmine ovaca u sjeverozapadnoj Turskoj. Na temelju rezultata, za navedena područja planiraju se preporuke veterinarima i uzgajivačima na osnovi kojih bi se unaprijedio njihov rad. U istraživanje je bilo uključeno 400 ovaca iz različitih područja sjeverozapadne Turske. Uzimani su uzorci vode (n = 5), biljaka (n = 5) i tla (n = 5). Utvrđena je značajno niža razina željeza u 8., 9. i 10. području. Najniža razina cinka ustanovljena je u 1. i 7. području, najniža razina bakra u 1., 3., 5. i 6. području, dok su najviše razine olova utvrđene u 9. i 10. području, a kadmija u 2., 5. i 6. području. Analiza uzoraka vode, biljaka i tla pokazala je da između različitih područja postoje značajne razlike u razini cinka i bakra. Visoke razine olova ustanovljene su u uzorcima vode i biljaka. Prijenosom ovih rezultata do veterinara i uzgajivača iz promatranih područja, ostvarit će se značajna poboljšanja u liječenju i kontroli bolesti uzrokovanih deficijencijom elemenata u tragovima. Isto se može očekivati i za bolesti uzrokovane parazitima, bakterijama i virusima u kojih navedene deficijencije imaju određenu ulogu

Doublecortin-like kinase 1 levels and oxidant status in Alzheimer's disease

Purpose: Alzheimer disease (AD) is a progressive neurodegenerative disease that affects the neurons in various parts of the central nervous system. Recently discovered protein Doublecort like kinase-1 (DCLK-1) is one of the microtubule-associated protein. Our goal is to investigate the relationship of the role of the DCLK-1 in AD disease and oxidative stress. Material and Methods: The study included Alzheimerdisease- diagnosed 60 patients admitted to the clinic with memory disorders, and 30 healthy subjects. In the serum of patient and control group, DCLK-1, tau protein and zinc levels were measured. To assess the presence of oxidative stress, malondialdehyde (MDA), protein carbonyl group (PCG), protein thiol groups (PTG), glutathione (GSH) and catalase levels were detected. Dementia level was staged with Mini-Mental State Examination (MMSE) and Dementia Clinical Staging Scale (CDR). Results: Serum DCLK-1 and tau levels were determined significantly higher in AD compared to the control group). In the group with AD, levels of MDA, and PCG levels were significantly higher and GSH, catalase levels were determined significantly lower. DCLK-1 and MDA levels were determined significantly higher in the group with severe AD compared to the group with mild AD. In AD group, a positive correlation between DCLK-1 and, CDR and MDA; and negative correlation was found between MMSE and B12 vitamin Conclusions: The presence of a relation with increase in DCLK1 levels in AD and risk factors shows that it can be a new marker in assessing the disease

Relationships Among Some Biochemical Parameters, Trace Elements and Lipid Peroxidation Levels in Women With Gestational Diabetes Mellitus

Aim:The aim of the present study was to determine levels of copper (Cu), zinc (Zn), iron (Fe), malondialdehyde and some biochemical parameters in women with gestational diabetes mellitus (GDM) compared with healthy pregnant women, and to evaluate the relationships among these parameters.Materials and Methods:The patients consisted of 56 pregnant with GDM. The control group consisted of 60 healthy pregnant women. Plasma malondialdehyde, blood glucose, insulin, glycosylated hemoglobin (HbA1c), total cholesterol, triacylglycerol and LDL-cholesterol were measured by biochemical methods. The concentrations of serum Fe, Cu and Zn were determined by flame atomic absorption spectrophotometer.Results:The levels of blood glucose, insulin, HOMA-IR, glycosylated hemoglobin (HbA1c), total cholesterol, triacylglycerol and LDL-cholesterol, levels of plasma malondialdehyde, levels of serum Fe and Cu in women with GDM group were found higher than those in healthy pregnant women. However, levels of serum Zn were significantly lower in GDM pregnants than those in the healthy pregnant women. There were significant negative correlations between LDL-cholesterol and Cu levels of GDM subjects. Additionally, there were significant negative correlation between LDL-cholesterol and plasma malondialdehyde levels. However, there were significant positive correlations between serum Fe and plasma malondialdehyde levels.Conclusion:Our findings emphasize the significant deficiencies of Zn levels and the significant elevation of malondialdehyde, hyperglycemia and hyperlipidemia in women with GDM. Therefore, supplementation with Zn element involved in the antioxidative system may increase antioxidative enzyme activities, and consequently, an improvement in fetal and maternal complications may be expected

Varikoseli Olan Hastalarda Kan Ve Seminal Plazma Çinko (Zn), Bakır (Cu), Demir (Fe), Kadmiyum (Cd), Nitrik Oksit (No) Ve Asimetrik Dimetilarginin (Adma) Düzeyleri Arasındaki İlişkinin Araştırılması

Amaç: Varikosel pleksus pampiniformis içerisindeki testikuler venlerin anormal dilatasyonu olarak tanımlanan ve günümüzde erkeklerde infertiliteye neden olan en sık görülen hastalıklardan biridir. Son yıllarda varikoselin patofizyolojisinde oksitatif stresin ilişkili olduğunu ileri süren çalışmalar bulunmaktadır. Çalışmamızda varikosel tanısı konan hastaların eş zamanlı olarak panpiniform pleksus içindeki varikoz damarlarından ve mediyan kübital venden alınan kan örnekleriyle beraber hastaların seminal plazmasında, NO, ADMA, Zn, Cu, Fe ve Cd düzeyleri ameliyat öncesi ve ameliyat sonrası ölçülerek bu parametreler arasındaki ilişki açıklanmaya çalışılacaktır. Materyal Metod: Varikoselli 29 hastadan ameliyat öncesi ve sonrası semen ve kan örnekleri alınarak çalışmaya dahil edildi. Cd, Zn, Cu ve Fe düzeyleri atomik absorpsiyon spektrofotometresi ile ölçüldü. Tüm örneklerin NO düzeyleri kolorimetrik yöntemle, ADMA düzeyleri ticari ELISA kiti ile ölçüldü. Sonuçlar: Seminal plazma ve spermatik vende operasyon sonrası NO azaldığı, ADMA arttıpı tespit edildi. Sperm motilitesi operasyon sonrası arttığı, eser element değerlerinin değişmediği görüldü. Tartışma: Sonuç olarak; Varikoselli hastalarda ameliyat öncesi artan oksidatif stresin ameliyat sonrası dönemde azalması sonucu sperm fonksiyonlarında iyileşme sağladığı söylenebilir.Aim: Varicocele is defined as an abnormal dilatation of veins within plexus pampiniformis and cause infertility in men today is one of the most common diseases. In the pathophysiology of varicocele suggesting that oxidative stress is associated with disease progression. In our study diagnosed with varicocele patients simultaneously panpiniform plexus in the varicose veins and the median cubital vein blood samples taken together with the patients' seminal plasma, NO, ADMA, Zn, Cu, Fe and Cd levels preoperatively and postoperatively by measuring these parameters, the relationship between will be explained. Materials and Method: Twenty-nine patients with varicocele before and after surgery semen and blood samples were included in the study. Cd, Zn, Cu and Fe levels were measured by atomic absorption spectrometry. NO levels of all samples by a colorimetric method, ADMA levels were measured using commercial ELISA kits. Results: Seminal plasma and spermatic vein NO levels were decreased, while ADMA levels were increased after the operation. Sperm motility increased after the operation, but the trace elements levels were no statistical differences between the groups. Conclusions: In conclusion, Increased oxidative stress in patients with varicocele before surgery reduced postoperative said that as a result of improvement in sperm function

Alzheimer hastalığında doublecortin-like kinaz-1 düzeyleri ve oksidan durumu

Purpose: Alzheimer disease (AD) is a progressive neurodegenerative disease that affects the neurons in various parts of the central nervous system. Recently discovered protein Doublecort like kinase-1 (DCLK-1) is one of the microtubule-associated protein. Our goal is to investigate the relationship of the role of the DCLK-1 in AD disease and oxidative stress. Material and Methods: The study included Alzheimerdisease- diagnosed 60 patients admitted to the clinic with memory disorders, and 30 healthy subjects. In the serum of patient and control group, DCLK-1, tau protein and zinc levels were measured. To assess the presence of oxidative stress, malondialdehyde (MDA), protein carbonyl group (PCG), protein thiol groups (PTG), glutathione (GSH) and catalase levels were detected. Dementia level was staged with Mini-Mental State Examination (MMSE) and Dementia Clinical Staging Scale (CDR). Results: Serum DCLK-1 and tau levels were determined significantly higher in AD compared to the control group). In the group with AD, levels of MDA, and PCG levels were significantly higher and GSH, catalase levels were determined significantly lower. DCLK-1 and MDA levels were determined significantly higher in the group with severe AD compared to the group with mild AD. In AD group, a positive correlation between DCLK-1 and, CDR and MDA; and negative correlation was found between MMSE and B12 vitamin Conclusions: The presence of a relation with increase in DCLK1 levels in AD and risk factors shows that it can be a new marker in assessing the disease

Effects of 12-week combined exercise therapy on oxidative stress in female fibromyalgia patients

The aims of this study were to investigate the effect of exercise therapy on the oxidative stress in fibromyalgia patients and relationship between oxidative stress and fibromyalgia symptoms. Thirty women diagnosed with fibromyalgia according to the American College of Rheumatology preliminary criteria, and 23 healthy women whose age- and weight-matched women were enrolled the study. Pain intensity with visual analog scale (VAS), the number of tender points, the fibromyalgia impact questionnaire (FIQ), the Beck depression inventory (BDI) were evaluated. The oxidative stress parameters thiobarbituric acid reactive substances, protein carbonyls, and nitric oxide, and antioxidant parameters thiols and catalase were investigated in patients and control group. After, combined aerobic and strengthen exercise regimen was given to fibromyalgia group. Exercise therapy consisted of a warming period of 10 min, aerobic exercises period of 20 min, muscle strengthening exercises for 20 min, and 10 min cooling down period. Therapy was lasting 1 h three times per week over a 12-week period. All parameters were reevaluated after the treatment in the patient group. The oxidative stress parameters levels were significantly higher, and antioxidant parameters were significantly lower in patients with fibromyalgia than in the controls. VAS, FIQ, and BDI scores decreased significantly with exercise therapy. The exercise improved all parameters of oxidative stress and antioxidant parameters. Also, all clinical parameters were improved with exercise. We should focus on oxidative stress in the treatment for fibromyalgia with the main objective of reducing oxidative load