2 research outputs found
Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities
The eco-friendly synthesis of metallic nanoparticles (MNPs) using biological materials is an encouraging and innovativeness approach to nanotechnology. Among other synthesizing methods, biological methods are chosen because of their high efficiency and purity in many aspects. In this work, using the aqueous extract obtained from the green leaves of the D. kaki L. (DK); silver nanoparticles were synthesized in a short time and simply with an eco-friendly approach. The properties of the synthesized silver nanoparticles (AgNPs) were characterized using various techniques and measurements. In the characterization data of AgNPs, Maximum absorbance at 453.34 nm wavelengths, the average size distribution of 27.12 nm, the surface charge of −22.4 mV, and spherical appearance were observed. LC-ESI-MS/MS analysis was used to assess the compound composition of D. kaki leaf extract. The chemical profiling of the crude extract of D. kaki leaves revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the identification of five major high-feature compounds: two major phenolic acids (Chlorogenic acid and Cynarin), and tree flavonol glucosides (hyperoside, quercetin-3-glucoside, and quercetin-3- D-xyloside). The components with the highest concentrations were cynarin, chlorogenic acid, quercetin-3- D-xyloside, hyperoside, and quercetin-3-glucoside, respectively. Antimicrobial results were determined by a MIC assay. The biosynthesized AgNPs exhibited strong antibacterial activity against the human and food pathogen Gram (+ and −) bacteria and good antifungal activity against pathogenic yeast. It was determined that 0.03–0.050 μg/mL concentrations ranges of DK-AgNPs were growth suppressive concentrations on all pathogen microorganisms. The MTT technique was used to study the cytotoxic effects of produced AgNPs on cancer cell lines (Glioblastoma (U118), Human Colorectal Adenocarcinoma (Caco-2), Human Ovarian Sarcoma (Skov-3) cancer cell lines, and Human Dermal Fibroblast (HDF) healthy cell line). It has been observed that they have a suppressive effect on the proliferation of cancerous cell lines. After 48 h of treatment with Ag-NPs, the DK-AgNPs were found to be extremely cytotoxic to the CaCo-2 cell line, inhibiting cell viability by up to 59.49% at a concentration of 50 g mL−1. It was found that the viability was inversely related to the DK-AgNP concentration. The biosynthesized AgNPs had dose-dependent anticancer efficacy. Because of the high concentration of bioactive chemicals in Diospyros kaki, it may be employed as a biological resource in medicinal applications. DK-AgNPs were shown to be an effective antibacterial agent as well as a prospective anticancer agent. The results provide a potential approach for the biogenic production of DK-AgNPs utilizing D. kaki aqueous leaf extract
Synthesis, characterization, antimicrobial and cytotoxic activities of silver nanoparticles synthesized from green leaves of Persimmon (Diospyros kaki) fruit
Bu çalışmada Cennet Hurması meyvesinin yeşil yaprakları ile elde edilen özüt ve 5 MilliMolar gümüş nitrat çözeltisi 5: 1 oranında cam bir kap içerisinde reaksiyona bırakıldı. Reaksiyon sonucunda nano partiküller (AgNP'ler) çevre dostu yöntemle ekonomik ve basit bir biçimde sentezlendi. Sentezlenen AgNP'ler UV-visiblespektrofotometre (UV-Vis.), Fourier Transform İnfrared Spectroscopy (FTIR), X- Işınımı Kırınımı Difraktrometresi(XRD), Taramalı Electron Mikroskobu (SEM), Zeta potansiyeli ve Zetasizer cihaz verileri kullanılarak karakterize edildi. Karakterizasyon verilerine göre 453 nm dalga boyunda maksimum absorbans, küresel görünüm, 7-20 nm arasında kristal nano boyuta sahip, -19.9 mV zeta potansiyeline sahip oldukları belirlendi. AgNP'lerin patojen gram negatif ve pozitif bakteriler ile mayanın üremeleri üzerinde baskılayıcı etkileri mikro dilusyon yöntemi ile Minimum İnhibisyon Konsantrasyonu (MİK) tespit edildi. Partiküllerin sitotoksik etkileri sağlıklı hücre hattı (HDF) ve kanserli hücre hatları (U118, CaCo-2 ve Skov-3) ile incelendi.In this study, the extract obtained from the green leaves of the Persimmon fruit and the 5 MilliMolar silver nitrate solution were reacted in a 5: 1 ratio in a glass container. As a result of the reaction, nanoparticles (AgNPs) were synthesized in an economical and simple way with an environmentally friendly method. The synthesized AgNPs were characterized using UV-visible spectrophotometer (UV-Vis.), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometry (XRD), Scanning Electron Microscopy (SEM), Zeta potential and Zetasizer instrument data. According to the characterization data, it was determined that they have a maximum absorbance at 453 nm wavelength, spherical appearance, crystal nano size between 7-20 nm, and a zeta potential of -19.9 mV. The suppressive effects of AgNPs on the growth of pathogenic gram-negative and positive bacteria and yeast were determined by the micro-dilution method, and the Minimum Inhibition Concentration (MIC) was determined. The cytotoxic effects of the particles were investigated with healthy cell line (HDF) and cancer cell lines (U118, CaCo-2 and Skov-3)