Efek Proteksi Kombinasi Minyak Wijen (Sesame Oil) Dengan α-Tocopherol Terhadap Steatosis Melalui Penghambatan Stres Oksidatif Pada Tikus Hiperkolesterolemia

Minyak wijen (MW) yang banyak mengandung polyunsaturated fatty acid (PUFA) berfungsi menurunkan kadar lipid serum melalui induksi β oksidasi di mitokondria. Proses ini menghasilkan produk sampingan berupa radikal bebas. Vitamin E (α-tocopherol) diketahui menghambat aktifitas radikal bebas. Penelitian ini bertujuan untuk membuktikan efek sinergisme MW dan α-tocopherol dalam menghambat steatosis yang diakibatkan keadaan hiperkolesterol. Hasil penelitian menunjukan bahwa diperoleh perbedaan bermakna (p<0,05) terutama dosis MW 1,2 ml pada semua parameter antara kelompok hiperkolesterol, MW dan MW+α-tocopherol. Kadar kolesterol kelompok hiperkolesterol (140,925±10,5) mg/dl; kelompok MW (93,845±4,37) mg/dl dan kelompok MW+α-tocopherol (92,90±8,5) mg/dl. Kadar trigliserida kelompok hiperkolesterol (154,78±11,09) mg/dl; kelompok MW (184,64±3,87) mg/dl dan kelompok MW+α-tochopherol (66,89 ± 6,03) mg/dl. Pada kelompok MW kadar MDA (0,257±0,013) nmol/mg protein, kelompok hiperkolesterol (0,847±0,036) nmol/mg protein dan kelompok kombinasi MW dengan α-tocopherol (0,092±0,006) nmol/mg protein. Kadar SOD pada kelompok kombinasi MW dengan α-tocopherol (253,82±16,63) U/mg protein, kelompok MW (208,7±11,27) U/mg protein, kelompok hiperkolesterol (139,7±2,82) U/mg protein. Dari gambaran histologis steatosis lebih banyak didapatkan pada kelompok hiperkolesterol yang disertai dengan keradangan, sedangkan pada kelompok kombinasi minyak wijen dengan α-tochopherol memiliki gambaran histologis terendah mengalami steatosis

Standardized Centella Asiatica Increased Brain- Derived Neurotrophic Factor and Decreased Apoptosis of Dopaminergic Neuron in Rotenone-Induced Zebrafish

Rotenone is a pesticide that is widely used to kill insects and nuisance fish in lakes. Its used as Parkinson’s Disease (PD) model inducer. The mechanism of toxicity of rotenone is primarily mediated by its potential as mitochondrial complex I inhibition. Centella asiatica (CA) is known as neurotonic, but how its potential protection in Parkinsonism is still unclear. In this study, we examined the effect of CA to Brain-derived Neurotrophic Factor (BDNF) as a neuroprotectant and apoptosis as hallmark of PD in rotenone-induced zebrafish (Danio rerio). Besides, we also measured the zebrafish motility and dopamine (DA) level in the brain. We used adult zebrafish (8 months). Its exposed to 5 g/L rotenone and co-incubated with methanolic extract of CA by several concentrations which are 2.5, 5 and 10 μg/mL for 28 days. Motility observed for 5 minutes at 0, 7, 14, 21 and 28 days. Measurement DA by ELISA, BDNF and apoptosis by immunohistochemistry. The results showed that CA significantly (p<0.05) increased motility and dopamine level in all concentration of extract. Interestingly, BDNF expression in 5 and 10 μg/mL groups had no significantly difference to the control group. Concentration 10 μg/mL could protect dopaminergic neuron from rotenone toxicity due to significantly (p<0.05) decreased compare to rotenone group. Together, these data suggest that methanolic extract of CA could protect Parkinsonian syndrome conserved dopaminergic neuron through increasing BDNF as neurotrophic factor