Novel strategies for assessing platelet reactivity

There are many approaches to assessing platelet reactivity and many uses for such measurements. Initially, measurements were based on the ability of platelets separated from other blood cells to aggregate together following activation with an appropriate ‘aggregating agent’. Later, measurements of platelet aggregation in blood itself were performed, and this led to a point-of-care approach to platelet function testing. Measurement of secretory activity through the appearance of the activation marker P-selectin on platelets now provides an alternative approach, which enables remote testing. Measurement of vasodilator-stimulated phosphoprotein phosphorylation is also moving toward application in situations remote from the testing laboratory. Here we provide an overview of the various approaches that are now available, assess their advantages and disadvantages, and describe some of the clinical situations in which they are being used

Calibration and validation of reference evapotranspiration models in semi-arid conditions

Reference evapotranspiration (ETo) is an important parameter for climatological, hydrological and agricultural management. The FAO56 Penman-Monteith (FAO56-PM) model is one of the most accurate models. But it needs a detailed climate dataset from weather stations. Therefore, empirical reference evapotranspiration models (ETo) that need a reduced set of climate data can become an alternative approach. In this study, nine different evapotranspiration models were calibrated for the 1978-2000 period and validated based on the period between 2001-2017 with respect to standard FAO56-PM method based on the real climatic data obtained from Aseer metrological department, Saudi Arabia. The ranking of all the evaluated models based on the multi-criteria decision making was done in order to get the best alternative to the FAO56-PM Model. The result showed that Mahringer and Trabert models are the most appropriate with RMSE values of 2.13 mm/day and 2.47 mm/day, respectively and the value of percent error were 77.27% and 89.43%, respectively. Moreover, the values of mean bias error were found to be-2.03 mm/day and-2.35 mm/day, respectively. The calibration and validation of different ETo equations tend to increase their performance. Thus, the validated evapotranspiration model that used less climatic parameters could predict the ETo condition accurately for any region

Using Spiritual Connections to Cope With Stress and Anxiety During the COVID-19 Pandemic

During the initial phases of the COVID-19 pandemic, stress and anxiety were pervasive among the masses due to high morbidity and mortality. Besides the fear of coronavirus was also particularly driven by social media. Many people started to look for faith and spiritual connections to gain comfort. The role of spiritual ties and religious beliefs in relation to coping with pandemic stress has acquired the attention of researchers in some parts of the world. This cross-sectional survey aimed at assessing the intensity of stress and anxiety symptoms experienced by people and how much they were alleviated by employing spiritual connections. The study sample comprises 795 respondents with 52% males and 48% females living in Saudi Arabia. The brief online study questionnaire collected data about background variables, anxiety and stress scale from DASS-21, and items from the WHOQOL (SRBP) instrument assessed the use of spiritual beliefs to cope. Multiple regression models were tested to determine the role of spiritual connections after adjusting demographic variables. Results illustrated that after adjusting for gender and age, participants’ anxiety symptoms decreased by (β = −0.27; p = 0.000) units with each unit increase in the use of spiritual connections, and participants’ stress symptoms reduce by (β = −0.36; p = 0.000) units with each unit increase in coping with spirituality. Additionally, females’ risk to experience anxiety and stress symptoms was more than males [(β = 0.88; p = 0.01) and (β = 0.92; p = 0.000)], respectively. An increase in age decreases the likelihood of experiencing anxiety symptoms and stress symptoms by (β = −0.75; p = 0.02) and (β = −0.11; p = 0.000) units, respectively. Findings support the protective role of spiritual connections despite small beta coefficients. The social and cultural context in Saudi Arabia favors deep-rooted connections with spirituality and faith. Our findings support the fact that the reliance on spiritual connections helped older people to deal with exaggerated fear during the initial phase of the COVID-19 pandemic and reduces the risk of experiencing anxiety and stress symptoms. Females and younger participants were relatively vulnerable to developing these symptoms. We discussed these findings considering some recent studies that reported similar relationships and made recommendations for future research

New insight into strategies used to develop long-acting G-CSF biologics for neutropenia therapy

Over the last 20 years, granulocyte colony-stimulating factors (G-CSFs) have become the major therapeutic option for the treatment of patients with neutropenia. Most of the current G-CSFs require daily injections, which are inconvenient and expensive for patients. Increased understanding of G-CSFs’ structure, expression, and mechanism of clearance has been very instrumental in the development of new generations of long-acting G-CSFs with improved efficacy. Several approaches to reducing G-CSF clearance via conjugation techniques have been investigated. PEGylation, glycosylation, polysialylation, or conjugation with immunoglobulins or albumins have successfully increased G-CSFs’ half-lives. Pegfilgrastim (Neulasta) has been successfully approved and marketed for the treatment of patients with neutropenia. The rapidly expanding market for G-CSFs has increased demand for G-CSF biosimilars. Therefore, the importance of this review is to highlight the principle, elimination’s route, half-life, clearance, safety, benefits, and limitations of different strategies and techniques used to increase the half-life of biotherapeutic G-CSFs. Understanding these strategies will allow for a new treatment with more competitive manufacturing and lower unit costs compared with that of Neulasta

The need for strategic research and study centers (think tanks) in the Kingdom of Saudi Arabia

Rapid growth in the Kingdom of Saudi Arabia (KSA) demands a transparent policy-making apparatus to meet emerging needs. Government agencies alone may not be able to devise legitimate public policies. The presence of an adequate number of capable think tanks in KSA will add legitimacy to the public policy-making process. Think tanks can provide policy guidance on modern issues like economic growth, resource allocation, job creation, unemployment reduction, financial management, legislation, and human capital development. The Majlis al Shura is a ministerial-level institution in today’s KSA and performs in a similar manner to think tanks. There is an increased demand to help launch independent research centers and provide them with needed support due to accelerated growth. The KSA government seems willing to extend its support to such institutes without governmental intervention and pressures. The Syrian issue generated a broad-based policy discussion in the United States and was taken as a case study. Many lessons can be drawn from this case that are specific to the local dynamics of KSA, and which can be employed in economics, security, foreign policy, social welfare, advancing national goals, protecting national interests, capturing market share in the global market, attracting foreign direct investment, and so on. Based on these lessons learned, this research proposes a way forward for encouraging the establishment of think tanks in KSAhttp://archive.org/details/theneedforstrate1094541345Saudi Arabian National GuardApproved for public release; distribution is unlimited

Investigating resistance of layers in nickel germanide formed on amorphous and crystalline germanium

Nickel germanide formed on amorphous and crystalline germanium was investigated for sheet resistance, resistivity and specific contact resistivity of A1 to the NiGe layers. This paper reports on electrical characterization of NiGe using both c-Ge and a-Ge