1 research outputs found
Simple Monitoring of Cancer Cells Using Nanoparticles
Here we present a new strategy for a simple and fast
detection
of cancer circulating cells (CTCs) using nanoparticles. The human
colon adenocarcinoma cell line (Caco2) was chosen as a model CTC.
Similarly to other adenocarcinomas, colon adenocarcinoma cells have
a strong expression of EpCAM, and for this reason this glycoprotein
was used as the capture target. We combine the capturing capability
of anti-EpCAM functionalized magnetic beads (MBs) and the specific
labeling through antibody-modified gold nanoparticles (AuNPs), with
the sensitivity of the AuNPs-electrocatalyzed hydrogen evolution reaction
(HER) detection technique. The fully optimized process was used for
the electrochemical detection of Caco2 cells in the presence of monocytes
(THP-1), other circulating cells that could interfere in real blood
samples. Therefore we obtained a novel and simple in situ-like sensing
format that we applied for the rapid quantification of AuNPs-labeled
CTCs in the presence of other human cells