Multistable Phase Regulation for Robust Steady and Transitional Legged Gaits

We develop robust methods that allow specification, control, and transition of a multi-legged robot’s stepping pattern—its gait—during active locomotion over natural terrain. Resulting gaits emerge through the introduction of controllers that impose appropriately-placed repellors within the space of gaits, the torus of relative leg phases, thereby mitigating against dangerous patterns of leg timing. Moreover, these repellors are organized with respect to a natural cellular decomposition of gait space and result in limit cycles with associated basins that are well characterized by these cells, thus conferring a symbolic character upon the overall behavioral repertoire. These ideas are particularly applicable to four- and six-legged robots, for which a large variety of interesting and useful (and, in many cases, familiar) gaits exist, and whose tradeoffs between speed and reliability motivate the desire for transitioning between them during active locomotion. We provide an empirical instance of this gait regulation scheme by application to a climbing hexapod, whose “physical layer” sensor-feedback control requires adequate grasp of a climbing surface but whose closed loop control perturbs the robot from its desired gait. We document how the regulation scheme secures the desired gait and permits operator selection of different gaits as required during active climbing on challenging surfaces

Gait Generation and Control in a Climbing Hexapod Robot

We discuss the gait generation and control architecture of a bioinspired climbing robot that presently climbs a variety of vertical surfaces, including carpet, cork and a growing range of stucco-like surfaces in the quasi-static regime. The initial version of the robot utilizes a collection of gaits (cyclic feed-forward motion patterns) to locomote over these surfaces, with each gait tuned for a specific surface and set of operating conditions. The need for more flexibility in gait specification (e.g., adjusting number of feet on the ground), more intricate shaping of workspace motions (e.g., shaping the details of the foot attachment and detachment trajectories), and the need to encode gait “transitions” (e.g., tripod to pentapod gait structure) has led us to separate this trajectory generation scheme into the functional composition of a phase assigning transformation of the “clock space” (the six dimensional torus) followed by a map from phase into leg joints that decouples the geometric details of a particular gait. This decomposition also supports the introduction of sensory feedback to allow recovery from unexpected event and to adapt to changing surface geometries

Rapid Pole Climbing with a Quadrupedal Robot

This paper describes the development of a legged robot designed for general locomotion of complex terrain but specialized for dynamical, high-speed climbing of a uniformly convex cylindrical structure, such as an outdoor telephone pole. This robot, the RiSE V3 climbing machine—mass 5.4 kg, length 70 cm, excluding a 28 cm tail appendage—includes several novel mechanical features, including novel linkage designs for its legs and a non-backdrivable, energy-dense power transmission to enable high-speed climbing. We summarize the robot’s design and document a climbing behavior that achieves rapid ascent of a wooden telephone pole at 21 cm/s, a speed previously unachieved—and, we believe, heretofore impossible—with a robot of this scale. The behavioral gait of the robot employs the mechanical design to propel the body forward while passively maintaining yaw, pitch, and roll stability during climbing locomotion. The robot’s general-purpose legged design coupled with its specialized ability to quickly gain elevation and park at a vertical station silently with minimal energy consumption suggest potential applications including search and surveillance operations as well as ad hoc networking

Biologically Inspired Climbing with a Hexapedal Robot

This paper presents an integrated, systems-level view of several novel design and control features associated with the biologically inspired, hexapedal, RiSE (Robots in Scansorial Environments) robot. RiSE is the first legged machine capable of locomotion on both the ground and a variety of vertical building surfaces including brick, stucco, and crushed stone at speeds up to 4 cm/s, quietly and without the use of suction, magnets, or adhesives. It achieves these capabilities through a combination of bioinspired and traditional design methods. This paper describes the design process and specifically addresses body morphology, hierarchical compliance in the legs and feet, and sensing and control systems that enable robust and reliable climbing on difficult surfaces. Experimental results illustrate the effects of various behaviors on climbing performance and demonstrate the robot\u27s ability to climb reliably for long distances

An Introduction to Sphingolipid Metabolism and Analysis by New Technologies

Sphingolipids (SP) are a complex class of molecules found in essentially all eukaryotes and some prokaryotes and viruses where they influence membrane structure, intracellular signaling, and interactions with the extracellular environment. Because of the combinatorial nature of their biosynthesis, there are thousands of SP subspecies varying in the lipid backbones and complex phospho- and glycoheadgroups. Therefore, comprehensive or “sphingolipidomic” analyses (structure-specific, quantitative analyses of all SP, or at least all members of a critical subset) are needed to know which and how much of these subspecies are present in a system as a step toward understanding their functions. Mass spectrometry and related novel techniques are able to quantify a small fraction, but nonetheless a substantial number, of SP and are beginning to provide information about their localization. This review summarizes the basic metabolism of SP and state-of-art mass spectrometric techniques that are producing insights into SP structure, metabolism, functions, and some of the dysfunctions of relevance to neuromedicine

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Farmers' bulletin (United States. Dept. of Agriculture); no. 1427

"A definite record of expenditures and receipts is one of the greatest needs of many poultry keepers. This bulletin gives a simple systems of keeping poultry accounts adapted either for a small flock or for a large poultry farm. Combined with this system of accounting is a detailed statement of expenditures and receipts and an inventory of the necessary equipment for operating a 1,500-hen commercial poultry farm. The records advised are simple, consisting of a monthly statement sheet, a yearly summary sheet, an inventory sheet, a balance sheet, and a daily egg record." -- p. i