Adipogenic differentiation.

<p>ASCs cultivated on biomaterials PEUU (A), PEU (B) and PLLA (C) and differentiated for 21 days into an adipogenic lineage. Cells are stained with AdipoRed to show fat droplets. Bars represent 100 µm.</p

Stress-Strain curves of PEU and PEUU foils in comparison to conventional PLLA foils.

<p>Stress-Strain curves of PEU and PEUU foils in comparison to conventional PLLA foils.</p

ASCs) were differentiated for seven and fourteen days on polymer-coated plates as well as on typical cell culture plates (TCP).

<p>Afterwards, expression of 17 genes associated with adipogenesis and lipogenesis was determined by qRT-PCR. All Ct values were normalized to GAPDH. Mean values of expression levels from 2 different donors are depicted relative to undifferentiated cells set as 1.</p

Cell viability of ASCs grown on biomaterials for one, three and seven days was detected by MTT Assay.

<p>One representative experiment out of four independent experiments is shown.</p

Molecular weights (M_n, M_w, M_z) and polydispersity indices of prepared oligomers and polymers determined by GPC

<p>Molecular weights (M_n, M_w, M_z) and polydispersity indices of prepared oligomers and polymers determined by GPC</p

Electrospinning parameters and resulting fiber diameters of the electrospun non-woven biomaterials (flow rate for all materials: 1.5 ml h⁻¹)

<p>Electrospinning parameters and resulting fiber diameters of the electrospun non-woven biomaterials (flow rate for all materials: 1.5 ml h⁻¹)</p

Cell viability and morphology.

<p>ASCs were grown on biomaterial PEUU (A-D), PEU (E-H) and PLLA (I-L) for 48 hours. Viability was detected by fluorescein diacetate (living cells; A, E, I) and ethidium-homodimer-1 (nuclei of dead cells; B, F, J) staining. C, G and K are merged pictures. Morphology was detected by TRITC-phalloidin (cytoskeleton, red) and DAPI (nuclei, blue) staining (D, H, L). Bars represent 100 µm (A-C, E-G, I-K) or 50 µm (D, H, L).</p

Degradation of compact samples of PEUU and PEU within 44 weeks in Sörensen phosphate buffer at 37°C.

<p>Degradation of compact samples of PEUU and PEU within 44 weeks in Sörensen phosphate buffer at 37°C.</p

Mechanical properties (tensile strength, Young’s modulus, and maximum elongation) and glass transition temperatures (T_g) of synthesized polymers (PEUU, PEU) in comparison to PLLA.

a<p>peakmaximum tan delta [°C] (measured by DMA); ^b half C_p (measured by DSC).</p

Adipogenic differentiation.

<p>ASCs cultivated on biomaterials PEUU (A), PEU (B) and PLLA (C) and differentiated for 21 days into an adipogenic lineage. Cells are stained with AdipoRed to show fat droplets. Bars represent 100 µm.</p