Total fatty acid distribution in <i>Pichia pastoris</i> cells grown in a hydrogenated environment at 30°C (red) and 18°C (green) and in a deuterated environment at 30°C (blue) and 18°C (cyan).

<p>Data represent mean values ± s.d (n = 3). In histograms, *<i>P</i><0.05 from Student's <i>t</i>-test, assuming equal variance. For C16:0, there is a significant difference between H 30°C and H 18°C, between H 30°C and D30°C, H 30°C and D18°C, between H 18°C and D 30°C but not between H18°C and D18°C. For C16:1, there is a significant difference for all 4 different temperatures and isotopic contents. For C16:2, there is a significant difference between D30°C and D18°C. For C18:1, there is a significant difference for all 4 different temperatures and isotopic contents. For C18:2, there is a significant difference between D30°C and H30°C, between D30°C and D18°C, between D30°C and H18°C. For C18:3, there is a significant difference for all 4 different temperatures and isotopic contents.</p

Ratio (upper part) of C16/C18 fatty acids and (lower part) of unsaturated/saturated fatty acid (UFA/SFA) when <i>Pichia pastoris</i> cells were grown in hydrogenated (H) and deuterated (D) media at 18 and 30°C.

a<p>: Total, total fatty acids; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; PG, phosphatidylglycerol; CL, cardiolipine.</p

GC-MS analysis of ergosterol isotopomers from <i>Pichia pastoris</i> unsaponifiable extracts.

<p>A, TIC of an acetylated extract from cells grown in hydrogenated medium. The peak at RT = 36.10 min is ergosterol. B, TIC of an acetylated extract from cells grown in deuterated medium. The peak at RT = 35.26 min is deuterioergosterol. C, mass spectrum of ergosteryl acetate. Prominent ions and interpretation of the fragmentation pattern: M⁺(438), M⁺-acetate-H (378), M⁺-acetate-H-CH₃ (363), M+-acetate-H-side chain (253). D, mass spectrum of deuterioergosteryl acetate. Prominent ions and interpretation of the fragmentation pattern: M⁺(481), M⁺-acetate-D (420), M⁺-acetate-D-CD₃ (402), M⁺-acetate-D-side chain (278).</p

Fatty acid distribution of the main phospholipids produced by <i>Pichia pastoris</i> cells grown in a hydrogenated medium at 30°C (red) and at 18°C (green) and in a deuterated environment at 30°C (blue) and 18°C (cyan).

<p>In all individual phospholipids, the deuterated environment triggers the enrichment in C18:1 fatty acids PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine. Errors bars represent the standard deviation from three different phospholipids extractions and separations. Data represent mean values ± s.d (n = 3). In histograms, *<i>P</i><0.05 from Student's <i>t</i>-test, assuming equal variance. PC: for C16:0, the difference is significant between H30°C and H18°C, between H30°C and D30°C and between H30°C and D18°C. For C16:1, there is a significant difference between H30°C and D30°C and between H18°C and D18°C. For C18:0, there is a significant difference between H18°C and D18°C, between H18°C and H30°C and between H18°C and D30°C. For C18:1, the difference is significant between all the different temperatures and isotopic contents. For C18:2, there is a significant difference between D30°C and D18°C, between D30°C and H30°C and between D30°C and H18°C. For C18:3, the difference is significant between all the different temperatures and isotopic contents. PE: for C16:0, the difference is significant between H30°C and D30°C, between H30°C and H18°C and between H30°C and D18°C. For C18:1 and C18:3, the difference is significant between all the different temperatures and isotopic contents. PS and PI: for C16:0, there is a significant difference between H30° and D30°C and between H30° and D18°C. For C16:2 and C18:1, there is a significant difference between H30°C and D30°C, between H18°C and D18°C and between D18°C and D30°C. For C18:2 and C18:3, there is a significant difference between H30°C and D30°C and between H18°C and D18°C.</p