Research Notes : U.S.S.R. : Subunit composition of glycinin from various samples of cultivated and wild soybean

The major storage protein of llS class of soybean seeds, glycinin, has a complex subunit structure. Each of the six subunits is composed of two pro-tein molecules (acidic and basic), linked via disulphide bonds (Badley et al., 1975). Depending on subunit, the acidic moiety molecular weight varies from 37,000 to 42,000, with one exception (m.w. 10,000)

High Fat Diet Prevents Over-Crowding Induced Decrease of Sex Ratio in Mice

Adaptive theory predicts that mothers would be advantaged by adjusting the sex ratio of their offspring in relation to their offspring's future reproductive success. In the present study, we tested the effect of housing mice under crowded condition on the sex ratio and whether the fat content of the diet has any influence on the outcome of pregnancies. Three-week-old mice were placed on the control diet (NFD) for 3 weeks. Thereafter the mice were allotted randomly to two groups of 7 cages each with 4, 6, 8, 10, 12, 14, and 16 mice in every cage to create increasing crowding gradient and fed either NFD or high fat diet (HFD). After 4 weeks, dams were bred and outcomes of pregnancy were analyzed. The average dam body weight (DBW) at conception, litter size (LS) and SR were significantly higher in HFD fed dams. Further, male biased litters declined with increasing crowding in NFD group but not in HFD. The LS and SR in NFD declined significantly with increasing crowding, whereas only LS was reduced in HFD group. We conclude that female mice housed under overcrowding conditions shift offspring SR in favor of daughters in consistent with the TW hypothesis and high fat diet reduces this influence of overcrowding

CHANGES IN THE PARAMETERS OF BLOOD LIPIDS AND CYTOKINES IN PATIENTS WITH PRIMARY OSTEOARTHRITIS DURING TREATMENT WITH ARTHRA

Objective: to study the time course of changes in the cytokine profile, the parameters of lipid peroxidation (LPO), antioxidant defense, and lipid spectrum during Arthra therapy in patients with primary osteoarthritis (POA). Subjects and methods. The study was conducted in 26 POA patients (aged 39.5+5.4 years) with knee and hip joint lesions in the absence of synovitis. The efficiency of treatment was evaluated by pain intensity and WOMAC index. The levels of proinflammatory cytokines, lipids, LPO primary and intermediate products, and antioxidant defense were compared in patients with PAO prior to and 12 and 24 weeks after Arthra therapy. Results. Positive changes in articular manifestations were determined after 24 weeks of therapy, by using the WOMAC scale. The mean concentration of C-reactive protein (CRP) was halved in the patients 24 weeks following the start of Arthra therapy. The blood levels of IL-1β, IL-6, IL-18, TNF-α decreased just after 12 weeks of therapy as compared to the baseline level and continued to decline by the end of the therapy. There were reductions in LPO primary and intermediate products in serum and red blood cells. There was activation of antiradical protection (increases in serum and red blood cell catalase activities and serum antioxidant activity and a decrease in the number of hemolyzed red blood cells). After a course of therapy, there were reduced blood concentrations of total cholesterol and low-density lipoprotein (LDL) cholesterol in patients with POA. Conclusion. The POA patients treated with Arthra displayed improvements in LPO profile and antioxidant defense and lower blood concentrations of cholesterol and LDL cholesterol along with a decline in the levels of the blood cytokines IL-1β, IL-6, IL-18, TNF-α, and CRP

Intensity of free radical processes in blood and oral liquid of patients with atopic dermatitis

The biochemical intensity markers of free radical processes in blood and oral liquid of patients with atopic dermatitis are presented in this article. To appraise the oxidative metabolism condition the biophysical parameters such as the flash maximum and. H2O2 area of induced chemiluminescence and the concentration of biomolecular oxidative modification's products which can react on thiobarbituric acid were studied.. In the course of the research it was found, that a significant increase of free radical processes was marked in the blood, while in the oral cavity it was less expressed changes. These changes allow us to speak about the prospects of the use of oral liquid as the material for the development of non-invasive methods for diagnosis of metabolic rates of the body

Research Notes : U.S.S.R. : Subunit composition of glycinin from various samples of cultivated and wild soybean

The major storage protein of llS class of soybean seeds, glycinin, has a complex subunit structure. Each of the six subunits is composed of two pro-tein molecules (acidic and basic), linked via disulphide bonds (Badley et al., 1975). Depending on subunit, the acidic moiety molecular weight varies from 37,000 to 42,000, with one exception (m.w. 10,000).</p

Intensity of free radical processes in blood and oral liquid of patients with atopic dermatitis

The biochemical intensity markers of free radical processes in blood and oral liquid of patients with atopic dermatitis are presented in this article. To appraise the oxidative metabolism condition the biophysical parameters such as the flash maximum and. H2O2 area of induced chemiluminescence and the concentration of biomolecular oxidative modification's products which can react on thiobarbituric acid were studied.. In the course of the research it was found, that a significant increase of free radical processes was marked in the blood, while in the oral cavity it was less expressed changes. These changes allow us to speak about the prospects of the use of oral liquid as the material for the development of non-invasive methods for diagnosis of metabolic rates of the body

Power absorption in the plasma ion source of a helicon type

The article presents the solution of the power absorption problem in the helicon-type plasma ion source. Ion source used now as an injector of the IAP NASU nuclear microprobe was chosen for calculations. Results were obtained for hydrogen and helium plasma. Cylindrical plasma source is placed in the external longitudinal (along the cylinder axis) uniform magnetic field. Working frequency of the source w is in the range of wci <w <wce <wpe and w = 2 ×p × f (rad/s) ; f = 27,12 MHz . The values of the uniform external magnetic field, when the power absorption is maximum, were obtained for various plasma densities