Towards efficient production of highly optically pure d-lactic acid from lignocellulosic hydrolysates using newly isolated lactic acid bacteria

This study presents the production of D-lactic acid with high enantiomeric purity using lignocellulosic hydrolysates from newly isolated lactic acid bacterial (LAB) strains. Six strains, 4 heterofermentative and 2 homofermentative, were investigated for their ability to grow and produce lactic acid on sugar beet pulp (SBP) hydrolysates, containing a mixture of hexose and pentose sugars. Among the strains tested, three were isolates designated as A250, A257 and A15, all of which belonged to the genus Leuconostoc. Only strain A250 could be reliably identified as Leuconostoc pseudomesenteroides based on cluster analysis of Maldi-ToF spectra. All strains produced D-lactic acid in the presence of SBP hydrolysates, but with varying optical purities. The homofermentative strains achieved higher D-lactic acid optical purities, but without assimilating the pentose sugars. Co-cultivation of the homofermentative strain Lactobacillus coryniformis subsp. torquens DSM 20005 together with the heterofermentative isolate A250 led to the production of 21.7 g/L D-lactic acid with 99.3 % optical purity. This strategy enabled the complete sugar utilization of the substrate. Nanofiltration of the SBP hydrolysate enhanced the enantiomeric purity of the D-lactic acid produced from the isolates A250 and A15 by about 5 %. The highest D-lactic acid concentration (40 g/L) was achieved in fed-batch cultures of A250 isolate with nanofiltered SBP, where optical purity was 99.4 %. The results of this study underline the feasibility of a novel isolate as an efficient D-lactic acid producer using lignocellulosic hydrolysates

Valorising agro-industrial wastes within the circular bioeconomy concept: The case of defatted rice bran with emphasis on bioconversion strategies

The numerous environmental problems caused by the extensive use of fossil resources have led to the formation of the circular bioeconomy concept. Renewable resources will constitute the cornerstone of this new, sustainable model, with biomass presenting a huge potential for the production of fuels and chemicals. In this context, waste and by-product streams from the food industry will be treated not as "wastes" but as resources. Rice production generates various by-product streams which currently are highly unexploited, leading to environmental problems especially in the countries that are the main producers. The main by-product streams include the straw, the husks, and the rice bran. Among these streams, rice bran finds applications in the food industry and cosmetics, mainly due to its high oil content. The high demand for rice bran oil generates huge amounts of defatted rice bran (DRB), the main by-product of the oil extraction process. The sustainable utilisation of this by-product has been a topic of research, either as a food additive or via its bioconversion into value-added products and chemicals. This review describes all the processes involved in the efficient bioconversion of DRB into biotechnological products. The detailed description of the production process, yields and productivities, as well as strains used for the production of bioethanol, lactic acid and biobutanol, among others, are discussed. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Batch and continuous lactic acid fermentation based on a multi-substrate approach

The utilisation of waste materials and industrial residues became a priority within the bioeconomy concept and the production of biobased chemicals. The aim of this study was to evaluate the feasibility to continuously produce L-lactic acid from different renewable substrates, in a multi-substrate strategy mode. Based on batch experiments observations, Bacillus coagulans A534 strain was able to continuously metabolise acid whey, sugar beet molasses, sugar bread, alfalfa press green juice and tapioca starch. Additionally, reference experiments showed its behaviour in standard medium. Continuous fermentations indicated that the highest productivity was achieved when molasses was employed with a value of 10.34 g·L−1·h−1, while the lactic acid to sugar conversion yield was 0.86 g·g−1 . This study demonstrated that LA can be efficiently produced in continuous mode regardless the substrate, which is a huge advantage in comparison to other platform chemicals. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Recent Advances in D-Lactic Acid Production from Renewable Resources: Case Studies on Agro-Industrial Waste Streams

U posljednje vrijeme proizvodnja biorazgradivih polimera privlači veliku pažnju kao alternativa plastici. Polimer mliječne kiseline pri tom naviše obećava, a može se primijeniti u različite svrhe, od pakiranja hrane do proizvodnje farmaceutskih proizvoda. Novija istraživanja pokazuju da D-mliječna kiselina ima ključnu ulogu u proizvodnji polimera mliječne kiseline otpornog na toplinu. Istovremeno, za smanjenje troškova proizvodnje treba koristiti obnovljive izvore. Svrha je ovog revijalnog prikaza sažeti trenutno stanje u proizvodnji D-mliječne kiseline pomoću fermentacije, s naglaskom na iskorištenje otpada i nusproizvoda. Također je dan prikaz modela protočnog razdvajanja. Osim toga, opisani su rezultati obrade kisele sirutke, pulpe sjemenki kave i hidrolizata rižinih ljuski kao alternativnih sirovina za proizvodnju D-mliječne kiseline.The production of biodegradable polymers as alternatives to petroleum-based plastics has gained significant attention in the past years. To this end, polylactic acid (PLA) constitutes a promising alternative, finding various applications from food packaging to pharmaceuticals. Recent studies have shown that D-lactic acid plays a vital role in the production of heat-resistant PLA. At the same time, the utilization of renewable resources is imperative in order to decrease the production cost. This review aims to provide a synopsis of the current state of the art regarding D-lactic acid production via fermentation, focusing on the exploitation of waste and byproduct streams. An overview of potential downstream separation schemes is also given. Additionally, three case studies are presented and discussed, reporting the obtained results utilizing acid whey, coffee mucilage and hydrolysate from rice husks as alternative feedstocks for D-lactic acid production

Organic fraction of municipal solid waste for the production of L-lactic acid with high optical purity

The organic fraction of municipal solid waste (OFMSW) is an abundant biowaste with great potential in the bioeconomy model. Previous reports have demonstrated that OFMSW hydrolysates are good substrates for lactic acid (LA) production. However, LA can exist in two enantiomeric forms (L- and D-) and most commercial LA applications require a high enantiomeric purity, typically of the L-isomer. Due to natural occurring bacteria in the waste, a mixture of D- and L-LA can form in the substrate, reducing the final enantiomeric purity of the product and limiting its commercial application. In the research reported in this article, hydrolysates from OFMSW were evaluated for the production L-LA with high enantiomeric purity. Firstly, a pre-treatment with monopolar electrodialysis membranes was implemented to remove the unfavourable D-LA in the hydrolysate. This step allowed the reduction in LA concentration and subsequent fermentations of the hydrolysate resulted in enantiomeric purities over 98%. At the pilot scale, a fermentation of the pre-treated hydrolysate, by B. coagulans A166, resulted in a final LA concentration of 61.1 g L−1 and a yield of 0.94 g g−1. The downstream of the process resulted on a LA recovery of 51.5% and a L-LA optical purity of 98.7%

Reduction in grey matter atrophy in patients with relapsing multiple sclerosis following treatment with cladribine tablets

Background and purpose Measures of atrophy in the whole brain can be used to reliably assess treatment effect in clinical trials of patients with multiple sclerosis (MS). Trials assessing the effect of treatment on grey matter (GM) and white matter (WM) atrophy are very informative, but hindered by technical limitations. This study aimed to measure GM and WM volume changes, using a robust longitudinal method, in patients with relapsing MS randomized to cladribine tablets 3.5 mg/kg or placebo in the CLARITY study. Methods We analysed T1-weighted magnetic resonance sequences using SIENA-XL, from 0 to 6 months (cladribine, n = 267; placebo, n = 265) and 6 to 24 months (cladribine, n = 184; placebo, n = 186). Mean percentage GM and WM volume changes (PGMVC and PWMVC) were compared using a mixed-effect model. Results More GM and WM volume loss was found in patients taking cladribine versus those taking placebo in the first 6 months of treatment (PGMVC: cladribine: -0.53 vs. placebo: -0.25 [p = 0.045]; PWMVC: cladribine: -0.49 vs. placebo: -0.34 [p = 0.137]), probably due to pseudoatrophy. However, over the period 6 to 24 months, GM volume loss was significantly lower in patients on cladribine than in those on placebo (PGMVC: cladribine: -0.90 vs. placebo: -1.27 [p = 0.026]). In this period, volume changes in WM were similar in the two treatment arms (p = 0.52). Conclusions After a short period of pseudoatrophy, treatment with cladribine 3.5 mg/kg significantly reduced GM atrophy in comparison with placebo. This supports the relevance of GM damage in MS and may have important implications for physical and cognitive disability progression. © 2022 The Authors

Extraction of Phenolic Compounds from Palm Oil Processing Residues and Their Application as Antioxidants

Nusproizvodi proizvodnje palminog ulja, i to pogača od palminih sjemenki, palmina vlakna, ljuske palminih sjemenki i prazni grozdovi palminog ploda, upotrijebljeni su za ekstrakciju polifenolnih spojeva. Među tim nusproizvodima je pogača od palminih sjemenki sadržavala najviše ukupnih fenolnih spojeva, i to 5,19 mg u g suhe tvari, izraženih kao ekvivalent galne kiseline, dok je najmanje imao prazni grozd palminog ploda, i to 1,79 mg/g. Radi optimiranja ekstrakcije fenola ispitani su sljedeći parametri: vrijeme ekstrakcije i omjer tekuće i krute tvari. Najveći ukupni udjel fenola od 5,35 mg/g pri omjeru tekuće i krute tvari od 40:1 tijekom ekstrakcije od 20 min imala je pogača od palminih sjemenki. Pomoću HPLC-DAD metode određeni su glavni fenolni spojevi iz nusprodukata proizvodnje palminog ulja. Pogača od palminih sjemenki sadržavala je najviše pirogalola, te 4-hidroksibenzojeve, galne i ferulinske kiseline. Prazni grozdovi palminog ploda i palmina vlakna bili su bogati hidroksibenzojevom kiselinom, dok je pirogalol bio dominantan sastojak ekstrakta ljuski palminih sjemenki. Svi su ekstrakti imali oksidacijsku aktivnost, koja je potvrđena DPPH analizom, te ispitana dodatkom ekstrakta suncokretovom ulju radi produljenja roka trajanja. Dodatkom 0,8 % ekstrakta pogače od palminih sjemenki povećalo se indukcijsko vrijeme suncokretovog ulja za više od 50 %. Rezultati istraživanja potvrđuju da je pogača od palminih sjemenki nusproizvod s dodanom vrijednošću koji se može upotrijebiti kao antioksidans u prehrambenoj industriji.The side streams derived from the palm oil production process, namely palm kernel cake, palm pressed fibre, palm kernel shells and empty fruit bunches, were evaluated as sources of phenolic compounds. Among these streams, kernel cake had the highest total phenolic content (in mg of gallic acid equivalents (GAE) per g of dry sample) with a value of 5.19, whereas the empty fruit bunches had the lowest value (1.79). The extraction time and liquid-to-solid ratio were investigated to optimize the phenolic extraction. Kernel cake exhibited the highest total phenolic content (5.35 mg/g) with a liquid-to-solid ratio of 40:1 during 20 min of extraction. The main phenolic compounds of the extracts deriving from all byproduct streams were also identified and quantified with HPLC-DAD. Pyrogallol, 4-hydroxybenzoic acid, gallic acid and ferulic acid were the main compounds found in kernel cake extracts. Empty fruit bunch and pressed fibre extracts were also rich in 4-hydroxybenzoic acid, while pyrogallol was the predominant compound in kernel shell extracts. All extracts showed antioxidant activity as it was indicated from the results of DPPH analysis and subsequently tested in sunflower oil aiming to prolong its shelf life. The addition of 0.8 % kernel cake extract increased the induction time of sunflower oil more than 50 %. According to the results obtained in this study, kernel cake extracts could be considered as a value-added co-product with a potential application as antioxidants in the food industry

Summary of Research: Collaboration Between Healthcare Professionals and People with Multiple Sclerosis to Develop Communication Tools to Improve the Standard of Multiple Sclerosis Care

: This is a summary of a previously published paper: Joint Healthcare Professional and Patient Development of Communication Tools to Improve the Standard of MS Care. It describes a collaboration between people with multiple sclerosis (PwMS) and healthcare professionals (HCPs) to identify challenges in multiple sclerosis (MS) care and design tools to improve communication during consultations

Large wood debris that clogged bridges followed by a sudden release: the 2019 flash flood in Catalonia

The aim is the reconstruction of the October 2019 flash flood, that was documented through extensive field work: rainfall (300 mm in just a few hours), flood marks, times of flood passage and witnesses' snapshots and reports, channel changes, log drift (20,000 trees) and woody debris at bridges, as well as large damage and six fatalities. The methods are: hydrological model built for the rainfall-runoff in the basin and the flood routing in the river, use of hydraulic principles such as flow at waterfalls, flow against obstacles (trees), etc. and finally 1D/2D free surface numerical models. The uppermost 100 km2 produced discharges of 700 m3/s (up to 50 m3/s/km2, locally). Three bridges failed, but their cascading failure (when one failure triggers the next one downstream) was not proved. The main channel widened more than 10 times, dragging away soil and vegetation like a bulldozer. The resulting large wood debris that clogged two bridges worsened the inundation. An anomalous flow downstream, probably a surge of around 1090 m3/s, due to the failure of a woody jam at a narrow bridge, took two lives. Water Authority is now warning flood planners that vegetated, torrential basins may cause catastrophic floods in the valley towns, if their narrow bridges are sensitive to woody debris.Catalan Water Authority and its Tarragona officers, Meteoprades and Fons Signatus, Joan March and a number of witnesses. The UPC's contribution was funded by the contract CTN2000029 of the “Agència Catalana de l′Aigua”. The UB's contribution has been developed within the framework of the AGORA project, funded by the "Agència Catalana de l′Aigua". Our thanks to the "Museu de la Vida Rural", in l′Espluga de Francolí, and all the citizens who contributed to the return of experience. The UIB's research has been supported by the Ministerio de Ciencia, Innovación y Universidades (CGL2017-82868-R and PID2020-113036RB-I00/AEI/10.13039/501100011033 research projects, which are partially supported by the European Regional Development Funds).Peer ReviewedPostprint (published version