Regulation of the Na,K-ATPase Gamma-Subunit FXYD2 by Runx1 and Ret Signaling in Normal and Injured Non-Peptidergic Nociceptive Sensory Neurons

Dorsal root ganglia (DRGs) contain the cell bodies of sensory neurons which relay nociceptive, thermoceptive, mechanoceptive and proprioceptive information from peripheral tissues toward the central nervous system. These neurons establish constant communication with their targets which insures correct maturation and functioning of the somato-sensory nervous system. Interfering with this two-way communication leads to cellular, electrophysiological and molecular modifications that can eventually cause neuropathic conditions. In this study we reveal that FXYD2, which encodes the gamma-subunit of the Na,K-ATPase reported so far to be mainly expressed in the kidney, is induced in the mouse DRGs at postnatal stages where it is restricted specifically to the TrkB-expressing mechanoceptive and Ret-positive/IB4-binding non-peptidergic nociceptive neurons. In non-peptidergic nociceptors, we show that the transcription factor Runx1 controls FXYD2 expression during the maturation of the somato-sensory system, partly through regulation of the tyrosine kinase receptor Ret. Moreover, Ret signaling maintains FXYD2 expression in adults as demonstrated by the axotomy-induced down-regulation of the gene that can be reverted by in vivo delivery of GDNF family ligands. Altogether, these results establish FXYD2 as a specific marker of defined sensory neuron subtypes and a new target of the Ret signaling pathway during normal maturation of the non-peptidergic nociceptive neurons and after sciatic nerve injury

Barodontalgia and Dental Barotrauma Among Scuba Divers

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ARTIREV ::vers une démocratisation de la bibliométrie...

Dans cet article, nous présentons les résultats des derniers cycles d’un programme de recherche en science de la conception (Design Science Research) qui a mené au développement du prototype d’un nouveau logiciel, effectivement utilisable par des chercheurs néophytes en bibliométrie, pour les aider à conduire leurs revues de littérature. L’artefact résultant de ce programme de recherche, résout certains problèmes identifiés dans les logiciels bibliométriques existants. Ces problèmes sont : (1) la nécessité d’être formé de manière approfondie en bibliométrie pour pouvoir effectivement utiliser ces logiciels pour effectuer une revue de littérature, (2) le nettoyage insuffisant des données utilisées, menant à des résultats peu robustes, et (3) des représentations graphiques, qui sont agréables visuellement, mais difficiles à comprendre et interpréter rapidement. Nous choisissons un logiciel bibliométrique bien documenté et utilisé dans la recherche en SI, et nous comparons les résultats obtenus à partir de ce logiciel avec ceux obtenus à l’aide de notre artefact / prototype. Nous effectuons cette comparaison par le biais d’observations objectives et également d’autres éléments plus subjectifs, extraits du retour d’expérience de cinq doctorants, néophytes en bibliométrie, qui ont servi de béta testeurs pour notre prototype

Prevalence of orofacial trauma and the attitude towards mouthguard use in handball players: A survey in Lorraine, France

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ARTIREV ::an integrated bibliometric tool to efficiently conduct quality literature reviews

The present research was not subsidized. However, further development of the ARTIREV platform is currently subsidized by BPI France through a “French Tech Emergence” grant No. DOS0194751/00. In the current context of scientific information overload, researchers and practitioners alike could benefit from integrated bibliometric-based software tools to help them conduct reviews of existing literature. Using a design science research approach, and two bibliometric techniques (co-citation analysis of cited references and bibliographic coupling of citing documents), we propose a detailed workflow to conduct literature reviews and an artefact – a software tool we name ARTIREV (ARTificial Intelligence and literature REViews) that we evaluate in the management and medical fields. We show that ARTIREV addresses some issues identified in existing bibliometric software. These issues in existing tools are (1) the need for extensive bibliometric training to be able to effectively use them, (2) data cleaning that is insufficient to obtain reliable results, and (3) graphical representations, which are visually pleasing, but often difficult to interpret. The software tool resulting from our work could support the conduct of literature reviews for all prospective users: researchers and practitioners; bibliometric experts and neophytes

Efficacy of BRS® and Alpron®/Bilpron® Disinfectants for Dental Unit Waterlines: A Six-Year Study

Biofilms in dental unit waterlines (DUWL) are a potentially significant source of contamination posing a significant health risk as these may come into contact with patients and dental staff during treatment. The aim of this study was to evaluate the microbiological quality of DUWL water treated by Biofilm-Removing-System® (BRS®) and Alpron®/Bilpron® disinfectant solutions for six years in a French university hospital. The microbiological quality of water supplied by 68 dental units—initially shock treated with BRS®, then continuously treated by Alpron® with sterile water during working days and Bilpron® during inactivity period, and combined with purging every morning and after each patient—was assessed biannually during six years for total culturable aerobic bacteria at 22 °C and 36 °C, Legionella sp., Pseudomonas aeruginosa, and total coliforms. A total of 628 samples were analyzed, 99.8% were compliant with extended microbiological levels, and we never detected pathogen bacteria like Legionella sp. and P. aeruginosa. Only one sample (0.2%) was noncompliant with the level of total culturable aerobic bacteria at 36 °C, which exceeded 140 colony forming units per mL. The protocol implemented in our university hospital gives excellent results and enables control of the microbiological quality of DUWL water in the long term