19 research outputs found
Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
<div><p>To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.</p></div
Correlation between the results of ELISA and immunochromatographic assay.
<p>Estimation of human IgE in the standard solutions by the QD-based immunochromatographic assay is strongly correlated with the results obtained using a commercial ELISA kit (R<sup>2</sup> = 0.9989).</p
Correlation between ELISA and immunochromatographic assay results for samples from 95 human subjects.
<p>The immunochromatographic assay is able to estimate total IgE in the serum of human subjects, with good correlation kit (R<sup>2</sup> = 0.9884) to the results of a commercial ELISA.</p
Estimation of total IgE by immunochromatographic assay: mean (n = 10) and standard deviation.
<p>Estimation of total IgE by immunochromatographic assay: mean (n = 10) and standard deviation.</p
Selecting the optimal concentration of anti-IgE-QD.
<p>The figure shows the relationship between fluorescence intensity in the test zone of the immunochromatographic assay and the concentration of anti-IgE-QD conjugate. A standard solution of IgE with a concentration of 200 kU/L was used as a sample.</p
Estimation of total IgE by immunochromatographic assay and ELISA: mean (n = 8) and standard deviation.
<p>Estimation of total IgE by immunochromatographic assay and ELISA: mean (n = 8) and standard deviation.</p
Change in fluorescence intensity with analysis time.
<p>The figure shows the relationship between the analysis time and the fluorescence intensity in the test zone of the immunochromatographic test system. A standard solution of IgE with a concentration of 200/L was used as a sample.</p
Fluorescence intensity in the test zone of an immunochromatographic test strip at different combinations of antibodies against human IgE.
<p>Fluorescence intensity in the test zone of an immunochromatographic test strip at different combinations of antibodies against human IgE.</p