11 research outputs found
Correlation between ELISA and immunochromatographic assay results for samples from 95 human subjects.
<p>The immunochromatographic assay is able to estimate total IgE in the serum of human subjects, with good correlation kit (R<sup>2</sup> = 0.9884) to the results of a commercial ELISA.</p
Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
<div><p>To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.</p></div
Correlation between the results of ELISA and immunochromatographic assay.
<p>Estimation of human IgE in the standard solutions by the QD-based immunochromatographic assay is strongly correlated with the results obtained using a commercial ELISA kit (R<sup>2</sup> = 0.9989).</p
Estimation of total IgE by immunochromatographic assay: mean (n = 10) and standard deviation.
<p>Estimation of total IgE by immunochromatographic assay: mean (n = 10) and standard deviation.</p
Estimation of total IgE by immunochromatographic assay and ELISA: mean (n = 8) and standard deviation.
<p>Estimation of total IgE by immunochromatographic assay and ELISA: mean (n = 8) and standard deviation.</p
Selecting the optimal concentration of anti-IgE-QD.
<p>The figure shows the relationship between fluorescence intensity in the test zone of the immunochromatographic assay and the concentration of anti-IgE-QD conjugate. A standard solution of IgE with a concentration of 200 kU/L was used as a sample.</p
Change in fluorescence intensity with analysis time.
<p>The figure shows the relationship between the analysis time and the fluorescence intensity in the test zone of the immunochromatographic test system. A standard solution of IgE with a concentration of 200/L was used as a sample.</p
Fluorescence intensity in the test zone of an immunochromatographic test strip at different combinations of antibodies against human IgE.
<p>Fluorescence intensity in the test zone of an immunochromatographic test strip at different combinations of antibodies against human IgE.</p
Comparison of the sigmoidal and exponential equations for determining the concentration of total IgE from the calibration curve.
<p>Comparison of the sigmoidal and exponential equations for determining the concentration of total IgE from the calibration curve.</p
Calibration curves for the determination of total IgE in human serum.
<p><b>A -</b> Calibration curve of the immunochromatographic assay for total IgE in human serum. The calibration curve for the determination of total IgE in human serum by using the developed immunochromatographic assay (n = 10) was obtained. <b>B</b> - Calibration curve of the ELISA for total IgE in human serum. The calibration curve for the determination of total IgE in human serum by using the Total IgE EIA (n = 8) was obtained.</p