Recovery of adiponectin from five different dried blood spot concentrations.

<p>Recovery of adiponectin from five different dried blood spot concentrations.</p

Adiponectin levels in boys and girls by demographic and clinical characteristics, N = 13,329.

<p>SD = standard deviation; CI = confidence interval.</p>a<p>Linear regression coefficient and 95% CI (age-adjusted, accounting for clustering by polyclinic).</p>b<p>Normal weight, overweight and obese as defined by the International Obesity Task Force BMI cut-offs. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071315#pone.0071315-Cole1" target="_blank">[44]</a>.</p>c<p>Centiles derived from NHANES European age and sex-specific thresholds (reference) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071315#pone.0071315-Fernandez1" target="_blank">[45]</a>.</p>d<p>Centile derived from sample.</p>e<p>Quartiles derived from NHSR age-and sex-specific thresholds <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071315#pone.0071315-McDowell1" target="_blank">[46]</a>.</p>f<p>Quartiles derived from sample.</p

Comparison of adiponectin levels measured in PROBIT with other studies in children of a similar age.

<p>SD = standard deviation; m = months;</p>a<p>median (interquartile range);</p>b<p>interquartile range;</p>c<p>95% CI.</p

Bland-Altman plot for insulin values from fresh minus frozen samples.

<p>The difference in fasted bloodspot insulin from fresh sample minus the same sample frozen for 8 weeks at −80°C.</p

Typical calibration curve.

<p>Whole bloodspot adiponectin standards at 3.75, 7.5, 15.0, 30.46 µg/ml.</p

Stability of adiponectin concentrations (at A. low, B. medium and C. high values) measured from dried blood spots and stored at −80°C for 30 months.

<p>Adiponectin concentrations of internal quality control (IQC) bloodspots (low, medium and high values) analysed at regular intervals over 30 months. For the ‘low’ IQC sample (Fig. 2A), the middle red line is the mean adiponectin concentration and the upper and lower red lines are the 95% reference range (calculated from the standard deviation of 24 replicates); the respective lines showing the mean and 95% reference range for the ‘medium’ (Fig. 2B) and ‘high’ (Fig. 2C) IQC samples are also shown.</p

Comparison of adiponectin concentrations measured from 50 paired plasma and whole bloodspot samples.

<p>Comparison of adiponectin concentrations measured from 50 paired plasma and whole bloodspot samples.</p

Stability of insulin concentrations measured from dried blood spots at −80°C for 8 weeks.

<p>Comparison of fasting insulin values for dried blood spot samples stored at <b>−</b>80°C for 8 weeks versus fasting insulin concentrations measured from dried blood spots prior to storage.</p

Stability of fasting insulin stored at −80°C for 26 months.

<p>Fasting insulin concentrations of three bloodspot samples analysed prior to freezing (time 0) and then at regular intervals over 26 months. For sample 1, the middle red line is the insulin concentration at time 0 and the upper and lower red lines are the 95% reference range (calculated from the standard deviation of 30 replicates of the time 0 value). For samples 2 and 3, the lines are coloured green and blue, respectively.</p

Bland-Altman plot for fasted bloodspot versus serum insulin assay in 50 paired samples.

<p>Bland-Altman plot for fasted bloodspot versus serum insulin assay in 50 paired samples.</p