5 research outputs found
Structured inquiry-based learning: Drosophila GAL4 enhancer trap characterization in an undergraduate laboratory course.
We have developed and tested two linked but separable structured inquiry exercises using a set of Drosophila melanogaster GAL4 enhancer trap strains for an upper-level undergraduate laboratory methods course at Bucknell University. In the first, students learn to perform inverse PCR to identify the genomic location of the GAL4 insertion, using FlyBase to identify flanking sequences and the primary literature to synthesize current knowledge regarding the nearest gene. In the second, we cross each GAL4 strain to a UAS-CD8-GFP reporter strain, and students perform whole mount CNS dissection, immunohistochemistry, confocal imaging, and analysis of developmental expression patterns. We have found these exercises to be very effective in teaching the uses and limitations of PCR and antibody-based techniques as well as critical reading of the primary literature and scientific writing. Students appreciate the opportunity to apply what they learn by generating novel data of use to the wider research community
A Study of Parton Fragmentation in Hadronic Z0 Decays Using Lambda-Lambdabar Correlations
The correlated production of Lambda and Lambdabar baryons has been studied
using 4.3 million multihadronic Zo decays recorded with the OPAL detector at
LEP. Di-lambda pairs were investigated in the full data sample and for the
first time also in 2-jet and 3-jet events selected with the k_t algorithm. The
distributions of rapidity differences from correlated Lambda-Lambdabar pairs
exhibit short-range, local correlations and prove to be a sensitive tool to
test models, particularly for 2-jet events. The JETSET model describes the data
best but some extra parameter tuning is needed to improve agreement with the
experimental results in the rates and the rapidity spectra simultaneously. The
recently developed modification of JETSET, the MOdified Popcorn Scenarium
(MOPS), and also HERWIG do not give satisfactory results. This study of
di-lambda production in 2- and 3-jet events supports the short-range
compensation of quantum numbers.Comment: 23 pages, LaTeX, 6 figures included plus 1 in colour, submitted to
Physics Letters
Structured Inquiry-Based Learning: Drosophila GAL4 Enhancer Trap Characterization in an Undergraduate Laboratory Course
We have developed and tested two linked but separable structured inquiry exercises using a set of Drosophila melanogaster GAL4 enhancer trap strains for an upper-level undergraduate laboratory methods course at Bucknell University. In the first, students learn to perform inverse PCR to identify the genomic location of the GAL4 insertion, using FlyBase to identify flanking sequences and the primary literature to synthesize current knowledge regarding the nearest gene. In the second, we cross each GAL4 strain to a UAS-CD8-GFP reporter strain, and students perform whole mount CNS dissection, immunohistochemistry, confocal imaging, and analysis of developmental expression patterns. We have found these exercises to be very effective in teaching the uses and limitations of PCR and antibody-based techniques as well as critical reading of the primary literature and scientific writing. Students appreciate the opportunity to apply what they learn by generating novel data of use to the wider research community
Structured Inquiry-Based Learning: <i>Drosophila</i> GAL4 Enhancer Trap Characterization in an Undergraduate Laboratory Course
<div><p>We have developed and tested two linked but separable structured inquiry exercises using a set of <i>Drosophila melanogaster</i> GAL4 enhancer trap strains for an upper-level undergraduate laboratory methods course at Bucknell University. In the first, students learn to perform inverse PCR to identify the genomic location of the GAL4 insertion, using FlyBase to identify flanking sequences and the primary literature to synthesize current knowledge regarding the nearest gene. In the second, we cross each GAL4 strain to a UAS-CD8-GFP reporter strain, and students perform whole mount CNS dissection, immunohistochemistry, confocal imaging, and analysis of developmental expression patterns. We have found these exercises to be very effective in teaching the uses and limitations of PCR and antibody-based techniques as well as critical reading of the primary literature and scientific writing. Students appreciate the opportunity to apply what they learn by generating novel data of use to the wider research community.</p></div