CISH for HPV16 and AQUA score correlation.

<p>CISH for HPV16 and AQUA score correlation.</p

Histogram of AQUA scores with cutpoint identifying very high expression of p16.

<p>Histogram of AQUA scores with cutpoint identifying very high expression of p16.</p

Progression-free survival according to P16 AQUA score.

<p>P16 AQUA score with a cutpoint at 244 in patients with pretreatment hemoglobin≥120 g/L.</p

Overall survival according to P16 AQUA score.

<p>P16 AQUA score with a cutpoint at 244 in patients with pretreatment hemoglobin≥120 g/L.</p

CISH for HPV16 and DAB score correlation.

<p>DAB: conventional p16 diaminobenzydene.</p

Univariate analysis: 5-year progression-free survival (PFS).

<p>ECOG PS: Eastern Cooperative Oncology Group Performance Status; RT: radiotherapy; CRT: chemoradiotherapy; Hb: hemoglobin; DAB: diaminobenzydene, CISH: chromogenic <i>in situ</i> hybridization</p><p>Univariate analysis: 5-year progression-free survival (PFS).</p

Characteristics of 89 patients with anal canal carcinoma.

<p>ECOG PS: Eastern Cooperative Oncology Group Performance Status; RT: radiotherapy.</p><p>Characteristics of 89 patients with anal canal carcinoma.</p

P16 expression by AQUA according to HPV subtype.

<p>P16 expression by AQUA according to HPV subtype.</p

Conventional RT-PCR and direct cDNA sequencing confirm the presence of <i>EGFRvIII</i> in glioblastoma FFPE tissue.

<p>A. Conventional RT-PCR failed for GBM 11, GBM 23 and GBM 19. GBM 9 is positive for <i>EGFRvIII</i>. EGFRvIII positive control: U87MGvIII (384 bp), negative control: U87MG (1184 bp) and no template control (NTC): water. B. Sequencing of GBM 9 cDNA confirms the presence of <i>EGFRvIII</i>. C. Real-time amplification plot showing EGFRvIII-positive OSCC patient (13A) and the positive control (U87MGvIII).</p

Specific detection of <i>EGFRvIII</i> mRNA by real-time RT-PCR.

<p>A. Real-time amplification plot of <i>EGFRvIII</i> transcript as detected in the U87MGvIII cell-line. U87MGvIII cells were used as a positive control, U87MG cells were used as negative control: water was used for no template control. B. Direct sequencing of U87MG and U87MGvIII cDNA confirms the presence of <i>EGFRvIII</i> transcript in U87MGvIII cells only. The broken arrow indicates the <i>EGFRvIII</i>-specific exon 1 and exon 8 junction. C. Linear dynamic range of the <i>EGFRvIII</i> real-time RT-PCR assay (100, 10, 1, 0.1 and 0.01 ng cDNA). D. <i>EGFRvIII</i> real-time RT-PCR assay amplification efficiency.</p