The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes

THE ROLE OF THE PHOSPHOINOZITIDES IN THE PROCESS OF PERFORMANCE OF EXCITATIONBY THE SOMATIC NERVES

The object of investigation: the nervous fibres of the ambulatory feet of the crab, the sciatic nerves of the rabbit and of the rat. The work is aimed at studying the role of the phosphoinozitides in the regulation of the calcium absorption by the somatic nerves with its funstional activity. For the first time it has been shown, that the depolarization is the factor, intensifying the phosphoinositide (PI) exchange. The investigation of the components composition of the PI-cycle of the somatic nerves with its funstional activity has been performed. The obtained results widen the representation about the molecular mechanisms of generation and performance of excitation, and are important for understanding the pathological processes with the nervous system damageAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio