1 research outputs found
Targeted Quantitative Screening of Chromosome 18 Encoded Proteome in Plasma Samples of Astronaut Candidates
This
work was aimed at estimating the concentrations of proteins
encoded by human chromosome 18 (Chr 18) in plasma samples of 54 healthy
male volunteers (aged 20–47). These young persons have been
certified by the medical evaluation board as healthy subjects ready
for space flight training. Over 260 stable isotope-labeled peptide
standards (SIS) were synthesized to perform the measurements of proteins
encoded by Chr 18. Selected reaction monitoring (SRM) with SIS allowed
an estimate of the levels of 84 of 276 proteins encoded by Chr 18.
These proteins were quantified in whole and depleted plasma samples.
Concentration of the proteins detected varied from 10<sup>–6</sup> M (transthyretin, P02766) to 10<sup>–11</sup> M (P4-ATPase,
O43861). A minor part of the proteins (mostly representing intracellular
proteins) was characterized by extremely high inter individual variations.
The results provide a background for studies of a potential biomarker
in plasma among proteins encoded by Chr 18. The SRM raw data are available
in ProteomeXchange repository (PXD004374)