10 research outputs found
Sequence coverages of some of the predicted venom gland proteins of <i>S</i>. <i>c</i>. <i>edwardsii</i> as revealed by the combined approach of MALDI and ESI tandem mass spectrometry.
<p>Protein sequences including specific domains are indicted by colored bars; below these, corresponding peptides identified by ESI (black lines) and MALDI (red lines) are indicated.</p
Sequence alignment of the metalloproteinases of the venom of <i>S</i>. <i>c</i>. <i>edwardsii</i>.
<p>Different colors indicate the unique peptides identified by tandem mass spectrometry of the corresponding proteins.</p
Sequence alignment of the serine proteinases of the venom of <i>S</i>. <i>c</i>. <i>edwardsii</i>.
<p>Different colors indicate the unique peptides identified by tandem mass spectrometry of the corresponding proteins.</p
Identification of proteins of the venom of <i>S</i>. <i>c</i>. <i>edwardsii</i> not predicted from the transcriptome analysis.
<p>Identification of proteins of the venom of <i>S</i>. <i>c</i>. <i>edwardsii</i> not predicted from the transcriptome analysis.</p
Presence of transmembrane domains and signal peptide for the annoted proteins identified in insoluble and soluble protein fractions.
<p>Presence of transmembrane domains and signal peptide for the annoted proteins identified in insoluble and soluble protein fractions.</p
Number of trophozoites or cysts recovered from Swiss mice gut after infection with <i>G</i>. <i>duodenalis</i> strains BHCF1 and Portland-1.
<p>Mice were inoculated with 1 x 10<sup>6</sup> trophozoites through intra-gastric route. Parasites were recovered from gut and counted in Neubauer chamber. Numbers correspond to average and standard deviation of parasite counting’s obtained for each group, from three independent experiments, with minimal number of 3 mice per group, in each experiment.</p
Classification of the identified proteins by molecular function (A), biological process (B) and protein class (C).
<p>This classification is suggested by gene ontology and Panther<sup>®</sup> and provides a general characterization of a group of proteins. The numbers presented in each graph correspond to the number of proteins.</p
2D protein map showing the spots of the soluble protein fraction (Proteins 1 to 429) and the numerical distribution of localized proteins.
<p>The protein identification correspondent to each number is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164946#pone.0164946.s003" target="_blank">S3 Table</a>.</p
2D protein map showing the spots of the insoluble fraction (Proteins 430 to 903) and the numerical distribution of localized proteins.
<p>The protein identification correspondent to each number is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164946#pone.0164946.s003" target="_blank">S3 Table</a>.</p
Consensus phylogenetic relationships of <i>G</i>. <i>duodenalis</i> with Bayesian posterior probabilities using a Markov chain Monte Carlo sampling technique, for <i>bg</i>, <i>tpi</i>, <i>and gdh</i> gene sequences of <i>G</i>. <i>duodenalis</i>.
<p>Markov chains were run for 6,000,000 iterations and the trees were sampled every 100 iterations. The GTR model was used with gamma correction. Sequences from <i>Giardia muris</i>, <i>Giardia microti and Giardia ardeae</i> were employed as outgroups.</p