1 research outputs found
RNA Aptamer against Autoantibodies Associated with Multiple Sclerosis and Bioluminescent Detection Probe on Its Basis
Nowadays,
there are no specific laboratory tests for establishing
the diagnosis of multiple sclerosis (MS). The presence of proteolytic
autoantibodies against myelin basic protein is now considered as a
characteristic feature of MS. New 2′-F-containing RNA aptamer
of high affinity and specificity to these antibodies was selected.
Covalent conjugate of this aptamer and Ca<sup>2+</sup>-regulated photoprotein
obelin was obtained for the first time and applied as a label in bioluminescent
microplate assay to detect target antibodies. The developed model
solid-phase microassay is simple, fast, and highly sensitive