Standard operating procedure for the analysis of major ions in hydrothermal fluids by ion chromatography

Repository containing the Standard Operating Procedures for the analysis carried out in the Giovannelli Lab at the University of Naples Federico II, Ital

Standard Operating Procedure for the analysis of trace elements in hydrothermal fluids by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

This SOP validates an inductively coupled plasma mass spectrometry (ICP-MS) procedure for the determination of biometals and trace elements in hydrothermal fluids and sediments. Hydrothermal fluids are aqueous solutions with a wide range of temperature, salinity, pH and trace elements that can be used by a set of microbial proteins containing redox-sensitive transition metals as their catalytic core. Due to the high variability of these samples, we have developed this protocol taking into account the special features of the matrices analyzed. Our methods focus primarily on a subset of trace metals, Trace metals such as Fe, Co, Ni, Mo, W, V and Cu referred to as biometals, used by biology as cofactors in proteins. An ICP-MS 7900 Agilent system was used, with calibration curves linear in the 0.01 to 100 μg/L concentration range