Multilevel synchronization of human β-cells networks

β-cells within the endocrine pancreas are fundamental for glucose, lipid and protein homeostasis. Gap junctions between cells constitute the primary coupling mechanism through which cells synchronize their electrical and metabolic activities. This evidence is still only partially investigated through models and numerical simulations. In this contribution, we explore the effect of combined electrical and metabolic coupling in β-cell clusters using a detailed biophysical model. We add heterogeneity and stochasticity to realistically reproduce β-cell dynamics and study networks mimicking arrangements of β-cells within human pancreatic islets. Model simulations are performed over different couplings and heterogeneities, analyzing emerging synchronization at the membrane potential, calcium, and metabolites levels. To describe network synchronization, we use the formalism of multiplex networks and investigate functional network properties and multiplex synchronization motifs over the structural, electrical, and metabolic layers. Our results show that metabolic coupling can support slow wave propagation in human islets, that combined electrical and metabolic synchronization is realized in small aggregates, and that metabolic long-range correlation is more pronounced with respect to the electrical one

Effect of internal surface curvature on steady axisymmetric shock waves

A dissertation submitted to the Faculty of Engineering and the Built Environment, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Master of Science in Engineering, 2017.The cardinal aspects of supersonic and hypersonic propulsion intake design involve understanding the internal shock wave structures forming therein. A study was conducted to explore the effects of internal surface curvature and entry deflection angle on steady axisymmetric shock waves. Very little is known about these influences with only Curved Shock Theory, produced by M¨ older, providing analytical insight directly after a curved shock wave. The shock waves and accompanying flow fields which were generated were studied via experimental and numerical means. Radius normalised internal radii of curvature of 1, 1.5 and 2 with entry deflection angles of 0◦, 4◦ and 8◦ were investigated between a Mach number range of Mach 2.4 and 3.6. Experimental results were produced using a blow down supersonic wind tunnel facility and were captured via shadowgraph and schlieren flow visualisation techniques. The numerical simulations were validated using the experimental results. A self similar curved shock wave shape equation was presented with an empirical model which uses flow Mach number and internal radius of curvature in order to produce the resulting curved shock shape. Curved Shock Theory streamlines were used to try predict the internal surfaces that produced the curved shocks but results did not correlate. This was due to extreme streamline curvature curving the streamlines when the shock angle approached the Mach angle. Very good agreement was however found between the theoretical and numerical streamlines at lower curvatures. The higher the internal surface curvature and entry deflection angle, the greater the flow fields were impacted. Steeper characteristics formed as a result, curving the shock wave more noticeably. Both the internal surface curvature and entry deflection angle were found to have an effect on the trailing edge expansion fans which then altered the shape of downstream shock wave structures. The highest curvature models produced steady double reflection patterns due the flow being turned in onto itself by the imposed internal surface curvature. The effects of conical and curved internal surfaces were explored for additional insight into the presence of flow-normal curvature and the curving of the attached shock waves.XL201

Building for a Zero Carbon future: Trade-off between carbon dioxide emissions and primary energy approaches

Growing urbanization is driving urban policy makers to adopt sustainable practices aimed to limit the environmental impact of buildings which are responsible for an estimated 36% of climate-changing gas emissions in European cities. In order to meet the ambitious emission reduction targets set by the EU it is essential to develop policy for CO2 emissions saving. This work investigates the regulations of European countries that introduce carbon compliance requirement as implementation of the EPBD such as UK, Ireland, Austria and some Eastern European countries. With reference to the typical consumption pattern of an Italian home, the paper analyses the current limits of primary energy, RES requirements and CO2 emissions, investigating the relations between EPnren and carbon dioxide emissions levels

Targeting hypoxia signaling pathways in angiogenesis

Oxygen (O2) supply is constantly maintained by the vascular network for a proper tissue oxygenation. Hypoxia is the result of an increased O2 demand and/or decreased supply and is common in both physiological conditions and human diseases. Angiogenesis is one of the adaptive responses to hypoxia and is mainly regulated by the hypoxia-inducible factors, HIFs. These heterodimeric transcription factors are composed of one of three O2-dependent alpha subunits (HIF-1, HIF-2, and HIF-3) and a constitutively expressed O2-insensitive subunit (HIF-1 beta). Among them HIF-1 alpha is the most characterized and its activity is tightly controlled. Under hypoxia, its intracellular accumulation triggers the transcription of several genes, involved in cell survival/proliferation, autophagy, apoptosis, cell metabolism, and angiogenesis. HIF pathway is also modulated by specific microRNAs (miRNAs), thus resulting in the variation of several cellular responses, including alteration of the angiogenic process. The pro-angiogenic activity of HIF-1 alpha is not restricted to endothelial cells, as it also affects the behavior of other cell types, including tumor and inflammatory/immune cells. In this context, exosomes play a crucial role in cell-cell communication by transferring bio-active cargos such as mRNAs, miRNAs, and proteins (e.g., VEGFA mRNA, miR210, HIF-1 alpha). This minireview will provide a synopsis of the multiple factors able to modulate hypoxia-induced angiogenesis especially in the tumor microenvironment context. Targeting hypoxia signaling pathways by up-to-date approaches may be relevant in the design of therapeutic strategies in those pathologies where angiogenesis is dysregulated

Monitoring of a micro-smart grid: Power consumption data of some machineries of an agro-industrial test site

For the management of a (micro)-smart grid it is important to know the patters of the load profiles and of the generators. In this article the power consumption data obtained through a monitoring activity developed on a micro-smart grid in an agro-industrial test-site are presented. In particular, this reports the synthesis of the monitoring results of 5 loads (5 industrial machineries for crop micronization, corncob crashing and other similar processes). How these data were used within a monitoring and managing scheme of a micro-smart grid can be found in (E. Fabrizio, V. Branciforti, A. Costantino, M. Filippi, S. Barbero, G. Tecco, P. Mollo, A. Molino, 2017) [1]. The data can be useful for other researchers in order to create benchmarks of energy use input appropriate energy demand values in optimization tools for the industrial sector