Does nitrogen fertilization affect the secondary structures of gliadin proteins in hypoallergenic wheat?

One of the macronutrients indispensable for plant growth and development is nitrogen (N). It is responsible for starch and storage protein (gliadins and glutenins) biosynthesis and, in consequence, influences kernels’ quality and yields. However, applying N-fertilizers increases gluten content in wheat, and it may intensify the risk of developing allergy symptoms in gluten-sensitive individuals. The purpose of our research was to analyse whether and how the elimination of N-fertilizers during the cultivation of wasko.gl− wheat (modified genotype lacking ω-gliadins) changes the secondary structures of gliadin proteins. To this aim, using the FT-Raman technique, we examined flour and gliadin protein extracts obtained from kernels of two winter wheat lines: wasko.gl+ (with a full set of gliadin proteins) and wasko.gl− (without ω-gliadin fraction) cultivated on two different N-fertilization levels—0 and 120 kg N·ha(−1). On the basis of the obtained results, we proved that nitrogen fertilization does not have a major impact on the stability of the secondary structures of gliadin proteins for wasko.gl− wheat line with reduced allergenic properties. Furthermore, the results presented herein suggest the possibility of increasing the stability of glutenin structures as a result of the N-fertilization of wasko.gl− wheat line, which gives hope for its use in the production of wheat articles devoted to people suffering from diseases related to gluten sensitivity

Factors influencing the occurrence of Fusarium mycotoxins in the grain of winter wheat

Fusarium mycotoxins are secondary metabolites, bio-synthesized by filamentous fungi of the genus Fusarium, which, due to their diverse toxicity and difficulty in their removal from food and animal feeds, are the subject of research by scientists around the world. The most important mycotoxins found in wheat are: deoxynivalenol (DON), zearalenone (ZEN), and the T-2 and HT-2 toxins. Bearing consumer safety in mind, the levels of these substances in grain, food and animal feeds are regulated by law. In order to meet the health safety requirements imposed on grain, it is important to minimise the risk of fungal infection at individ-ual production stages. Weather conditions are the most important factor influencing the development of fungi and the accumula-tion of mycotoxins in grain. High temperature, combined with rainfall, are factors favouring the spread of fungal spores. Apart from weather conditions, individual elements of the production technology, such as appropriate variety selection, soil cultivation method, preceding crop, use of fungicides, and fertilization may reduce the quantity of mycotoxins in grain. In the conditions of the emerging climatic changes, favouring the development of fun-gal diseases, creating wheat varieties resistant to fungal diseases is a considerable challenge. Acquiring more in-depth knowledge on the relationship between the phenotypic features of a wheat variety and mycotoxin content would be helpful. This work is of review character and aims to indicate the basic factors influenc-ing the production of fusarium mycotoxins in winter wheat grain. The focus was placed on factors independent of human activity (weather conditions) and on individual elements of cultivation technology

Bread making potential of Triticum aestivum and Triticum spelta species

The aim of this study was to assess the effect of two winter wheat species: Triticum aestivum ssp. vulgare, and Triticum spelta, cultivated in different percentage of cereals in crop rotation, on their bread making potential. The analyzed grain samples were obtained from a field experiment conducted during three years 2014-2016 at the Experimental Station in Osiny (51°35’, 21°55’), Institute of Soil Science and Plant Cultivation, State Research Institute, Puławy, Poland. The experiment was established following different percentages of shared cereals in crop rotation (SCCR): 50, 75 and 100%. Wheat cultivation was performed in accordance with good agricultural practice standards. Protein content, gluten content, Zeleny sedimentation index and alveograph properties were determined. Wheat species strongly influenced bread potential. The Triticum aestivum compared to Triticum spelta was characterized by significantly higher gluten quality and Zeleny sedimentation index as well as better alveograph properties. Common wheat showed the highest baking value (W). Spelt wheat was characterized by the lowest tenacity (P) and the highest extensibility (L). Moreover, the percentage of cereals in crop rotation had an effect on grain and dough quality parameters. Cultivation of wheat in 100% share of cereals resulted in the lowest grain and dough quality

Bioactive Compounds in Different Buckwheat Species

The accumulation of valuable nutrients in cereal grains depends on a number of factors, including species, cultivars, and environment conditions. The aim of this study was to compare protein, some polyphenols and rutin content, as well as mineral composition in Fagopyrum tataricum and Fagopyrum esculentum genotypes growing in Polish conditions. A field experiment was conducted on pseudopodsolic soil in 2017–2018 at the Experimental Station in Osiny (51°35′, 21°55′), following randomized complete block method with three replications. Two cultivars of Fagophyrum esculentum (Kora and Panda), two cultivars of Fagopyrum tataricum (LIT1 and 63181) and two forms of Fagopyrum esculentum (Red Corolla and Green Corolla) were used in this experiment. We found differences in the tested compounds (protein, phenolic acids, rutin, and mineral composition) between cultivars and genotypes. Total phenolic acid and rutin contents were higher in the Fagopyrum tataricum compared to Fagopyrum esculentum. Ferulic and coumaric acids were prominent in the Kora and Panda cultivars, however vanillic and syringic acids accumulated more in Green Corolla and Red Corolla. The common buckwheat seeds contained more Cu, Mn, and Mg and less Ca than tartary buckwheat. Moreover Fagopytum esculentum genotype contains more protein compared to Fagopyrum tataricum

A Novel Fractional-Order RothC Model

A new fractional q-order variation of the RothC model for the dynamics of soil organic carbon is introduced. A computational method based on the discretization of the analytic solution along with the finite-difference technique are suggested and the stability results for the latter are given. The accuracy of the scheme, in terms of the temporal step size h, is confirmed through numerical testing of a constructed analytic solution. The effectiveness of the proposed discrete method is compared with that of the classical discrete RothC model. Results from real-world experiments show that, by adjusting the fractional order q and the multiplier term ζ(t,q), a better match between simulated and actual data can be achieved compared to the traditional integer-order model

Factors Influencing the Accumulation of Free Asparagine in Wheat Grain and the Acrylamide Formation in Bread

Asparagine is one of the precursors of acrylamide that can form during bread production. The aim of this work was to determine the effect of genotype, environment, sulfur fertilization, and the interaction of those factors on the asparagine content, technological value of wheat, and acrylamide level in bread. The research material consisted of five wheat cultivars grown in two locations in Poland with nitrogen fertilization of 110 kg ha−1 and sulfur fertilization of 30 kg ha−1. The standard ISO method for analyzing the milling and baking properties of wheat was used. The UHPLC-MS/MS method for analyzing the amino acids and the GC/MS method for acrylamide in bread were implemented. The analysis of variance results indicated that the location influenced the total variance in the measured asparagine content and quality of wheat the most, followed by the cultivar and then by the interaction between the environment and cultivar. Sulfur fertilization had no significant effect on the asparagine content, but slightly lowered the gluten quality and loaf volume of bread. However, sulfur fertilization in connection with the cultivar characterized by low starch damage had a positive effect on lowering the acrylamide in bread. Asparagine content in wheat and acrylamide in bread varies mostly depending on cultivar and environment

Analysis of Outcomes of the Nutritional Status in Patients Qualified for Aortic Valve Replacement in Comparison to Healthy Elderly

Severe aortic stenosis (AS) is associated with the reduction of muscle mass and may be associated with deterioration of nutritional status. Furthermore, malnourished cardiac patients are characterized by a higher risk of postoperative complications and mortality. The aim of this study was the evaluation and comparison of nutritional status, appetite and body composition in older people with severe aortic stenosis before aortic valve replacement and healthy elderly volunteers. One hundred and one patients, aged >65 years old with severe AS were included in the study. Nutritional status was assessed. Body composition was estimated using bioelectrical impedance analysis. Concentrations of albumin, prealbumin, triglycerides, total cholesterol and C-reactive protein were measured, and a complete blood count was done. About 40% of AS patients were at risk of malnutrition. They had decreased hand grip strength and they lost more body mass than the control group. Malnourished AS patients were older, had lower body mass indexes (BMIs) and lower aortic valve areas in comparison to well-nourished patients. Older AS patients, like their peers, show excessive body mass and, at the same time, the features of malnutrition. They have additional factors such as unintentional weight lost and decreased muscle strength which may be associated with worse outcomes

Multi-temporal phenological indices derived from time series Sentinel-1 images to country-wide crop classification

Crop classification is a crucial prerequisite for the collection of agricultural statistics, efficient crop management, biodiversity control, the design of agricultural policy, and food security. Crops are characterized by significant change during the growing season, and this information can be used to improve classification accuracy. However, capturing variation in vegetation cover requires a reliable source of valid data. Sentinel-1 radar images are a good candidate, as they supply information about Earth’s surface every six days, independent of weather and light conditions. In this paper, we present a method for crop classification based on radar polarimetry. We propose a set of multi-temporal indices derived from time series Sentinel-1 images that aim to characterize crop phenology. A big data, object-oriented classification technique is developed and tested on 16 crop types for the whole of Poland. Our analysis found that overall accuracy varied (regionally) from 86.36 to 89.13% in 2019, and from 85.95 to 89.81% in 2020. F1 scores for individual crops varied from 0.73 to 0.99, and the use of our multi-temporal phenological indices increased F1 scores by about 0.14 compared to calculations using only basic parameters. Results obtained for the whole country demonstrate the efficacy of the method and its resistance to environmental conditions