Astragalus glycyphyllos L.: Phytochemical constituents, pharmacology, and biotechnology

Astragalus glycyphyllos is a widely distributed plant found in Bulgaria that has been used in the folk medicine of the country for decades as an antihypertensive, diuretic, and anti-inflammatory. This review article is focused on the traditional usage, phytochemical studies, pharmacological activity, and biotechnology of this species. Recent progress in the phytochemical investigation led to the identification of different flavonoids, triterpenoid saponins, sterols, volatiles, etc. Many pharmacological studies, performed on extracts and pure compounds, revealed promising antiproliferative, cytotoxic, immunomodulatory, antiviral, and neuroprotective activities in vitro and an in vivo hepatoprotective effect on a model of CCl4-induced liver damage. Based on its popularity in traditional Bulgarian medicine, the species represents a promising subject for further investigations

Essential oil in Phlomis tuberosa

Phlomis tuberosa L. (Lamiaceae) is a flowering perennial plant, native to Eurasia, and sparsely distributed in Bulgaria. The species has been proved to accumulate flavonoids, iridoids, phenolic glycosides, as well as essential oil. To now, there is no information on the chemical content of Bulgarian essential oil from the plant. The aim was to study the chemical composition of essential oils, obtained from the species. Four different samples were studied – essential oils produced from leaves and herbs, collected in the fields, and from leaves and flowering parts, harvested from the mountainous location. It was found that β-copaene was the major in the essential oil from both the leaves and of the flowering parts collected from Popitza. In addition, the other major constituent in leaf essential oil from Kladnitza was tau-muurolol. The essential oil, produced from the species, harvested from the higher altitude had phytol as the main component. The sesquiterpenoid content in leaf essential oil suggests that this plant substance is appropriate for medicinal use, due to the pharmacological properties of these compounds

Beneficial effects of the fructus Sophorae extract on experimentally induced osteoporosis in New Zealand white rabbits

Sophora japonica is a source of several flavonol, flavone and isoflavone glycosides that are reported to positively affect menopausal symptoms including osteoporotic complications. In the present study fructus Sophorae extract (FSE) was administered orally for three months at a dose of 200 mg kg–1 in ovariectomized (OVX) New Zealand rabbits. 3D computed tomography scans and histopathological images revealed microstructural disturbances in the bones of the castrated animals. FSE recovered most of the affected parameters in bones in a manner similar to zoledronic acid (ZA) used as a positive control. The aglycones of the main active compounds of FSE, daidzin, and genistin, were docked into the alpha and beta estrogen receptors and stable complexes were found. The findings of this study provide an insight into the effects of FSE on bone tissue loss and suggest that it could be further developed as a potential candidate for the prevention of postmenopausal osteoporotic complications

Liquid chromatography – high resolution mass spectrometry screening of Astragalus hamosus and Astragalus corniculatus

Astragalus hamosus and Astragalus corniculatus were examined for the presence of flavoalkaloids, acylated and highly glycosylated flavonoids. Non-purified extracts of the overground parts of the species were subjected to ultra-high performance liquid chromatography – high resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) analysis and the results were compared to authentic reference substances. A flavoalkaloid of kaempferol was newly identified in an extract of A. hamosus. In addition, three compounds – quercetin and kaempferol flavonoids, acylated with hydroxymethylglutaric acid and alcesefoliside, were found in extracts of A. hamosus and A. corniculatus for the first time

Flavonoids in in vitro cultures of Astragalus hamosus

Astragalus hamosus contains valuable biologically active compounds, incl. flavonoids. The possibility for in vitro cultivation of the species as a source of important flavonoids was studied. Shoot and callus cultures were established and successfully cultivated on different nutrition media, complemented or not with growth regulators. An ultra-high performance liquid chromatography – high-resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) qualitative and quantitative analysis of non-purified methanol extracts of these cultures was performed. It was found that the cultures produced rutin in comparable quantity. Interestingly, both shoots and callus cultures accumulated the rare triglycosides alcesefoliside and mauritianin. The quantity of mauritianin, biosynthesized in shoots, was significantly higher to that in callus cultures. Alcesefoliside, was in lower quantity, compared to mauritianin. In addition, callus cultures produced alcesefoliside trice as the shoots, besides their lower level of differentiation. These findings could serve as initial research to establish the value of in vitro cultures from A. hamosus as an alternative mean of production of pharmaceutically important flavonol glycosides

Essential oil in Phlomis tuberosa

Phlomis tuberosa L. (Lamiaceae) is a flowering perennial plant, native to Eurasia, and sparsely distributed in Bulgaria. The species has been proved to accumulate flavonoids, iridoids, phenolic glycosides, as well as essential oil. To now, there is no information on the chemical content of Bulgarian essential oil from the plant. The aim was to study the chemical composition of essential oils, obtained from the species. Four different samples were studied – essential oils produced from leaves and herbs, collected in the fields, and from leaves and flowering parts, harvested from the mountainous location. It was found that β-copaene was the major in the essential oil from both the leaves and of the flowering parts collected from Popitza. In addition, the other major constituent in leaf essential oil from Kladnitza was tau-muurolol. The essential oil, produced from the species, harvested from the higher altitude had phytol as the main component. The sesquiterpenoid content in leaf essential oil suggests that this plant substance is appropriate for medicinal use, due to the pharmacological properties of these compounds

Toxicological and pharmacological profile of Amanita muscaria (L.) Lam. – a new rising opportunity for biomedicine

Amanita muscaria, commonly known as fly agaric, is a basidiomycete. Its main psychoactive constituents are ibotenic acid and muscimol, both involved in ‘pantherina-muscaria’ poisoning syndrome. The rising pharmacological and toxicological interest based on lots of contradictive opinions concerning the use of Amanita muscaria extracts’ neuroprotective role against some neurodegenerative diseases such as Parkinson’s and Alzheimer’s, its potent role in the treatment of cerebral ischaemia and other socially significant health conditions gave the basis for this review. Facts about Amanita muscaria’s morphology, chemical content, toxicological and pharmacological characteristics and usage from ancient times to present-day’s opportunities in modern medicine are presented

In vitro production of flavonoids in cultures of Gypsophila glomerata

Effects of increased concentration of calcium chloride on growth and production of flavonoids in newly established shoot and callus Gypsophila glomerata cultures were studied. The highest impact of CaCl2 on the growth index was determined in callus cultures (GI = 0.92), while in shoot cultures calcium treatment reduced the amount of biomass (GI = 0.38). Total flavonoids in shoot cultures grown on MS medium and MS medium supplemented with double amount of CaCl2 were 0.36 mg/g d. w. In both callus cultures, 2 mg/g d. w. total flavonoids were quantified. Shoots and callus grown on non-modified media accumulated 0.02 mg/g d. w. quercetin derivatives. Unlike these, both shoots and callus grown on calcium-enriched media accumulated 0.03 and 0.05 mg/g d. w. of isorhamnetin derivatives. In vitro shoot cultures grown on MS medium enriched in twice the amount of CaCl2 accumulated the highest amount of saponarin (0.138 mg/mg d. w.)

Activity of an oleanane-type tritrepenoid saponin from A. glycyphyllos on human recombinant MAO enzymes

For centuries, plants have been a leading point in the identification of potential therapeutic agents. Monoamine oxidase inhibition is a key mechanism in the treatment of various neurological and psychiatric diseases. Some triterpenoid saponins are reported to inhibit this enzyme. An extract from the aerial parts of Astragalus glycyphyllos was purified and separated by chromatographic techniques, which led to the isolation of one triterpene saponin. Its structure was analysed by ultra-high-performance liquid chromatography coupled with high-resolution electrospray ionisation mass spectrometry. The compound was subjected to a pharmacological study where human recombinant monoamine oxidase enzymes type A and B (hMAOA and hMAOB) were used. On the activity of hMAOA, the saponin had no effect, but on hMAOB, it exhibited statistically significant inhibition in comparison to the control, Selegiline. The compound could have potential in other models, so further investigations are required

A Study on the Safety and Effects of <i>Amorpha fruticosa</i> Fruit Extract on Spontaneously Hypertensive Rats with Induced Type 2 Diabetes

Metabolic syndrome is characterized by a variety of diagnostic criteria: obesity, dyslipidemia, type 2 diabetes, and arterial hypertension. They contribute to the elevated risk of cardiovascular morbidity and mortality. The potential for Amorpha fruticosa L. (Fabaceae) to improve diabetes and metabolic disease is promising, based on in vitro tests. This is why a further investigation of the species is needed. Additionally, a toxicity review in relation to safety revealed that to date, there are no published data regarding the toxicity of A. fruticosa towards humans. This species could provide abundant and cheap resources because it is an aggressive invasive plant that grows almost unrestrictedly. The objective of this study was to evaluate the acute toxicity of a purified extract of A. fruticosa (EAF), and to assess its antioxidant, antihypertensive, and antihyperglycemic activity in streptozotocin-induced diabetic spontaneously hypertensive rats (SHRs). The EAF was slightly toxic (LD50 = 2121 mg/kg, b.w.) when administered orally, and moderately toxic (LD50 = 316 mg/kg, b.w.) at intraperitoneal administration, both in mice. The oral administration of EAF (100 mg/kg) for 35 days to SHRs caused significant decreases in the systolic pressure, blood glucose levels, and MDA quantity. It also increased the hepatic level of the endogenous antioxidant GSH, not only in diabetic SHRs, but also in the control group. An additional potential benefit to human health might be conferred through the environmental management of A. fruticosa based on its large-scale use for medicinal purposes, as this aggressive invasive species brings problems to natural habitats in many European countries