Morphometric analyses of hepatic lesions from <i>E</i>. <i>cuniculi</i> infected mice.

<p>Lesions´ area of the liver from animals treated or not with cyclophosphamide (Cy) and treated or not with STZ to development of Type 1 Diabetes mellitus (DM).</p

Photomicrographs of histopathological lesions in <i>E</i>. <i>cuniculi Infected</i> and <i>DM-Infected</i> mice.

<p>Liver—mononuclear inflammatory infiltrate located at A) parenchyma, B) portal vein, C) under capsule and D) <i>E</i>. <i>cuniculi</i> clusters into inflammatory infiltrate. Lungs–E and F) interstitial pneumonia. Kidney–G) pyelonephritis and H) nephritis (HE).</p

IL-6, IFN-γ and TNF-α cytokines levels in the serum of Cy immunosuppressed mice and STZ-induced DM mice infected with <i>E</i>. <i>cuniculi</i> compared with its controls.

<p>+ and–means presence and absence, respectively, of STZ treatment and <i>E</i>. <i>cuniculi</i> infection. One way variance analysis (ANOVA) with Tukey <i>posttest</i> showed * p<0,05, *** p<0,001 and **** p<0,0001.</p

Spleen immune cell analysis.

<p>Evaluation of B-2 (CD23⁺CD19⁺) cells, CD4⁺ (CD19^-CD8^-CD4+), and CD8⁺ (CD19^-CD4^-CD8⁺) T lymphocytes and macrophages (CD19^-F4/80⁺CD11b⁺) in spleen Cy immunosuppressed and STZ-induced DM mice infected with <i>E</i>. <i>cuniculi</i> compared with its controls. Two ways variance analysis (ANOVA) revealed * p<0,05.</p

Peritoneal immune cell analysis.

<p>Evaluation of B-1 (CD23^-CD19⁺), B-2 (CD23⁺CD19⁺), Pre-B-1CDP (CD19⁺CD11b⁺F4/80⁺) cells, CD4⁺ (CD19^-CD8^-CD4+), CD8⁺ (CD19^-CD4^-CD8⁺) T lymphocytes and macrophages (CD19^-F4/80⁺CD11b⁺) from PerC of STZ-induced DM mice infected with <i>E</i>. <i>cuniculi</i> compared with its controls. Two ways variance analysis (ANOVA) revealed * p<0,05.</p

IL-6, IFN-γ and TNF-α cytokines levels in the serum of STZ-induced DM mice infected with <i>E</i>. <i>cuniculi</i> compared with its controls mice.

<p>+ and–means presence and absence, respectively, of STZ treatment and <i>E</i>. <i>cuniculi</i> infection. Two ways variance analysis (ANOVA) revealed * p<0,05 and **** p<0,0001.</p

Blood glucose, body weight and fungal burden parameters in mice immunosuppressed with cyclophosphamide (Cy): <i>Cy-Infected</i>, <i>Cy-Uninfected</i>, <i>DM-Cy-Uninfected</i> and <i>DM-Cy-Infected</i>.

<p>A) Blood glucose variation between immunosuppressed groups during all experimental period. B) Body weight variation of <i>DM-Cy-Infected</i> and Cy-<i>Infected</i> mice and its respective controls. C) Fungal burden in the PerC of <i>E</i>. <i>cuniculi</i> infected mice. + and–means presence or absence, respectively, of Cy treatment and <i>E</i>. <i>cuniculi</i> infection x means time folds compared to <i>Uninfected</i> (-/-) group. Non parametric <i>t-test</i> showed * p<0,05 and ** p<0,01 (A e B) and One way variance analysis (ANOVA) revealed $ p<0,05 comparing with uninfected groups and ** p<0,01 compared to the other groups.</p

Blood glucose, body weight and fungal burden parameters in mice of experimental groups: <i>Infected</i>, <i>Uninfected</i>, <i>DM-Uninfected</i> and <i>DM-Infected</i>.

<p>A) Blood glucose variation between groups during all experimental period. B) Body weight variation of <i>DM-Infected</i> and <i>Infected</i> mice and its respective controls. One way variance analysis (ANOVA) with Tukey post test showed *** p < 0,01. C) Fungal burden in <i>Infected</i> and <i>DM-Infected</i> mice by <i>E</i>. <i>cuniculi</i>. Non-parametric <i>t-test</i> showing * p<0.01, comparing each group of each period, separately. D) <i>E</i>. <i>cuniculi</i> spores observed in the PerC of <i>DM-Infected</i> mice stained by Calcofluor fluorescent staining.</p