1 research outputs found
Biochemical Study and Molecular Approaching on Eight Wild Herbals Used in Folk Medicine in Al-Baha Region, KSA
studies describing the traditional therapeutic uses of natural medicinal and aromatic plants have been established in Al-Baha region, KSA. In this work, the antioxidant activity of eight herbals plant samples from Al-Baha region was studied using different solvents (ethanol, hexane and water) to evaluate their strong and total lipid-soluble antioxidant and water-soluble antioxidant activity (TLAC37,95 and TWAC37,95). The eight herbal plants were also molecularly characterized using the PCR-based ISSR markers. The studied samples demonstrated valuable and variable antioxidant properties. The strong antioxidant capacity of Rumex (Rumex nervosus Vahl.) samples for ethanolic and aqueous extract samples was the highest followed by Fringed Rue (Ruta chalepensis L.) samples (47.68 and 42.72 & 81.74 and 74.18 µmoles/g respectively), while hexanic extracts of Euryops (Euryops arabicus Steud. ex Jaub. & Spach) samples revealed the highest strong antioxidant capacity followed by Fringed Rue samples (23.67 and 13.83 µmoles/g respectively). Besides that, ethanolic extracts of Fringed Rue samples demonstrated the highest total antioxidant capacity followed by olive leaves (Olea europaea subsp. cuspidata (Wall. & G.Don) Cif.) samples (686.95 and 462.62 µmoles/g respectively), while aqueous extracts of Rumex samples revealed superior total antioxidant capacity followed by olive leaves samples and Fringed Rue samples (869.10, 837.21 and 743.75 µmoles/g respectively. But the hexanic extracts of Fringed Rue samples revealed valuable total antioxidant capacity followed by Rumex samples (127.83 and 38.00 µmoles/g respectively. The herbal plants of Rumex, Fringed Rue and olive revealed huge both total and strong antioxidant activity. In the present study we began an initial approach to authenticate and identify different plant species in our study using inter-simple sequence repeats (ISSRs) molecular markers