Patients characteristics.

<p>nd: not done.</p><p>*: viral load below the limit of detection.</p><p>Patients characteristics.</p

Phylogenetic tree in the C2-V5 <i>env</i> region for LTNP patients and HIV-1 reference viruses.

<p>Sequences in the C2-V5 region in <i>env</i> gene, obtained by limiting dilution, from the three DI patients, the matched single infected individuals LTNP 17–1 (filled dark blue triangle),17–2 (empty dark blue triangle), and 17–3 (light blue triangle) <i>GeneBank accession number KC 595042–595055</i>, LTNP 2–1 (purple triangle), 2–3 (pink triangle) <i>GeneBank accession number KC595056-595080</i>, LTNP 20–1 (green triangle) <i>GeneBank accession numbers (KC595081-2 and KC 595237)</i>, LTNP 21–2 (filled grey triangle), 21–4 (empty grey triangle) and 21–5 (grey diamond) and 21–6 (grey square) (<i>GeneBank accession numbers KC 59546–48</i>, and <i>KT203901</i>-<i>13)</i> and LTNP 3–2 (yellow triangle) (<i>GeneBank accession numbers KT203872-80)</i> and sequences from reference viruses, LTNP and chronic progressor Spanish patients were included in the analysis. Overall, 180 sequences were subjected to phylogenetic analysis using the Maximum Composite Likelihood method in the MEGA5 program. Two sequences from subtype B viruses (89SP061 and HXB2 with <i>GeneBank accession numbers AJ006287 and K03455</i>, respectively) were included (black star). A subtype D sequence (D.CD.83.ELI, <i>GeneBank accession number</i> K03454I) indicated by grey star was also included as out-group. Sequences were obtained from the LANL database (<a href="http://www.hiv.lanl.gov/" target="_blank">http://www.hiv.lanl.gov</a>). Only bootstrap values above 80% are marked. Double infected patients are included in a box and with the following symbols MDM-3 (green circle), MDM-4 (empty green circle), MDM 5 (light green circle),MDM 6 (green square) <i>GeneBank accession numbers KC 594991–595006</i>, LTNP-5–1 (yellow circle), 5–2 (empty yellow square), 5–3 (filled yellow circle), 5–4 (empty yellow diamond), 5–6 (yellow diamond), 5–7 (empty light yellow diamond), 5–9 (yellow square) <i>GeneBank accession numbers K595120-145</i> and <i>KT203881-90</i> LTNP-15-1 (red circle) and 15–3 (empty red circle) (<i>GeneBank accession numbers KT203891-900</i>). Bar corresponds to genetic distance.</p

Clinical parameters and neutralization breadth comparison between Dual vs Single-infected patients.

<p>Six different viruses form 5 different subtypes were used (VI191 subtype A, two subtype B viruses NL4.3 and AC10, 92BR025 subtype C, 92UG024 subtype D, CM244 subtype E). In brackets is represented the subtype and the tier. Numbers indicate the percentage of reduction in infectivity ± SD after neutralization. Neutralization levels are indicated as follows: black square >90%, dark grey square 90–70%, light grey square 70–50% and white square <50% reduction in infectivity. Number of cross-reactive HIV-1-subtypes neutralized by two samples plasma from each patient are represented. Median of cross-reactive HIV-1-neutralizing activity by dual and single infected patient was compared. A statistical analysis was performed with the non-parametric a 2-tailed Mann-Whitney U test with a restrictive significance at the 95% using GraphPad Prism V 4.0 software.</p

Correlation between clinical data and genetic diversity parameters with heterologous NAb response.

<p>Association between viral load (panel A), Cd4+T cells (panel B), years after infection (panel C), Shannon Entropy (panel D), p-distance (panel E), number of synonymous mutations (ds) (panel F), number of non-synonymous mutations (dn) (panel G) and the ratio ds/dn (Panel H) and number of cross-reactive subtypes was calculated. Median of the different clinical data and the genetic parameters for each patient (y axis) was plotted against the number of subtypes neutralized by each the patients (x axis). Patients are identified by the following symbols. LTNP 17 (blue triangle), LTNP 2 (purple triangle), LTNP 20 (green triangle), LTNP 21 (grey triangle), LTNP 3 (orange triangle), MDM (green circle), LTNP-5 (yellow circle), and LTNP 15 (red circle). Correlation between parameters was calculated by linear regression with a 95% of confidence using GraphPad Prism V 4.0 software. For the comparison of the values, a statistical analysis was performed with the non-parametric 2-tailed Mann-Whitney U test with a restrictive significance at the 95% using GraphPad Prism V 4.0 software.</p