Regulation of class I expression by classical and inverse cim.

<p>(A) CD68+ cells were stained on the cell surface with OX18 (anti-class Ia and Ib). Histograms show representative samples from DA, DA.1H and DA.1H derived strains with different alleles of <i>RT1-A</i> and <i>Tap2</i> as stated on top. Data from all individuals are shown in scatterplot (far right); * significant compared to DA.1H (1H); ** significant compared to DA. (B) CD68+ cells stained with a class Ia specific antibody (F16-4-4). (C) T cells from animals shown in (A) stained intracellularly with OX18 (scatterplot) and F16-4-4 (histogram). (D–E) Subsets of leukocytes from DA and DA.1IR85 spleen stained extracellularly (D) and intracellularly (E) with OX18. Data are representative of 6 individuals per group. (F) Surface expression of MHC class I (OX18) on CD68+ cells from DA and DA.1U congenic strains. (G) CD68+ cells (same as in F) stained with F16-4-4. Vertical lines in scatterplots show mean values. Representative results of at least two independent experiments are shown.</p

<i>Tcs1</i> and <i>Tcs2</i>-congenic strains show similar variation in extracellular MHC expression in thymus and spleen.

<p>(A) Thymic conventional DCs (CD103+, CD11b/c+) stained extracellularly for class I (OX18). Scatterplots show results from two different experiments with <i>Tcs1</i> and <i>Tcs2</i>-congenic strains. The variation between the strains is comparable to data shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004151#pgen-1004151-g005" target="_blank">Figure 5</a> for CD68+ cells. (B–C) Splenic DCs (CD103+, CD68+, CD11b/c+) (B) and thymic DCs (C) stained extracellularly for RT1-D (OX17) and RT1-B (OX6). Histograms show representative samples from DA (solid lines) and DA.1HR10 (dashed lines).</p

CD4∶CD8 T cell ratio and extracellular MHC class I and II expression in spleens of congenic and recombinant strains.

a<p>RT1 haplotype designations are based on genotyping data (see <i>Methods</i>);</p>b<p>Strains were compared in separate experiments to DA littermate controls. The mean fluorescent intensity was then normalized to the expression of DA, which was given an arbitrary value of 100.</p>c<p>MHC-I (class Ia and Ib; OX18) extracellular expression on WBCs.</p>d<p>MHC class II expression on B cells. </p><p>Significant differences compared to DA:</p>e<p><i>P<0.001</i>;</p>f<p><i>P<0.01</i>;</p>g<p><i>P<0.05</i>.</p><p>WT, wildtype; PS, parental strain; RCS, recombinant congenic strain. Shown are mean values ±SD.</p

Physical map of the rat MHC region.

<p>The map was constructed according to the NCBI build 3.4 genome assembly. Genes (left) are depicted according to scale (positions in Mb), except for positions indicated with crotchets. The gross organization of MHC-Ia, II, III and Ib -regions are adopted from Hurt et al. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004151#pgen.1004151-Hurt1" target="_blank">[9]</a>. Recombinant congenic strains are shown as gray vertical bars with dashed lines representing congenic borders (intervals of unknown genotype). Markers, short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), are shown in italic numbers. Numbers with asterisks at the top and bottom of the figure represent the position of the closest negative (DA) marker. Genes in <i>Tcs1</i> are red and in <i>Tcs2</i> blue (see box for definitions). Inset shows the organization of the human (HLA) and rat (RT1) MHC regions. Non-recombinant congenic strains, which have fragments spanning the entire MHC region, are not shown. xMHC-II, extended MHC class II region; xMHC-I, extended MHC class I region.</p

Recombination hotspots and haplotype blocks in the rat MHC.

<p>Recombination activity was assessed over 2(3.4–5.4 Mb). Blue lines represent cold regions (haplotype blocks) with low recombination activity. Regions with recombinations are depicted as red bars. The width of the bars, and the table inset (right), represent recombination intervals. Numbers above bars in bold face represent the observed numbers of recombinations within the interval with the number of analyzed meiotic events shown below. The height of the bars indicate individuals with recombinations (in %). Blue triangles represent recombination hotspots in humans and are adopted from Cullen et al. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004151#pgen.1004151-Cullen1" target="_blank">[6]</a>.</p

Class-I modification reduces negative selection of CD8 cells.

<p>(A) The QTLs in <i>Tcs1</i> and <i>Tcs2</i> did not affect the total number of double negative cells (DN; CD4−, CD8b−). B cells (CD45RA+) were excluded from the DN gate. (B) DN cell maturation is defined by CD45RC and CD2 (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004151#pgen.1004151.s010" target="_blank">Fig. S10</a>). DN cells in DA.1FR9 showed a lower frequency of early thymic precursors (ETP) compared to DA. Counter plot shows gating strategy with numbers indicating percent (%) of parent population (stated above plot). (C) Counter plot shows CD45RC−, CD2^hi DN cells from DA.1UR83, and scatter plots the frequency of TCRβ− and TCRβ+ (DN4 in mouse) cells in DA and DA.1UR83. (D) Thymi from <i>Tcs2</i>-congenic strains with TAP-B (HR10 and UR10) contained fewer double positive (DP) cells but more cells (in %) with high TCR expression. (E) TAP-B strains (HR10 and UR10) showed higher frequencies (histograms) and total numbers (scatter plots) of CD8 single positive (SP) cells with high TCR expression. Numbers (%) in histograms represent mean-values ±SD of cells with high TCR expression (gated, <i>n</i> = 5). (F) Frequencies (histograms; <i>n</i> = 5) and total numbers (scatter plot) of CD8SP cells with high TCR expression in DA.1H (RT1-A^h, TAP-B) and in strains with low levels of surface MHC class I (HR83 [RT1-A^h, TAP-A] and IR85 [RT1-Aⁿ, TAP-A]). (G) Virtually all CD4SP cells express high levels of TCR. Histogram shows expression of TCR on CD4SP thymocytes in DA (<i>n</i> = 5). Scatterplot shows total number of CD4SP cells per thymus in <i>Tcs2</i>-congenic strains.</p

Genetic mapping in heterogeneous stock (HS) rats.

<p>(A) Genome scans of the CD4∶CD8 ratio (green), and the surface expression of MHC class I (MHC-I, red) and MHC class II (MHC-II, blue). The vertical axis shows the negative log <i>P</i> value for the 20 autosomes. Note that the left and right axis show −log <i>P</i> values for different phenotypes. Q1–Q10 indicate significant QTLs. (B) Close-up for Q8–Q10 on chromosome 20 showing the approximate location of the MHC region. (C) Position (in megabases, Mb) of peak marker (Pos.), confidence intervals (CI) in Mb, and effect sizes (%) of the peak markers in the QTLs shown in (A). The RMIP value is a measure of the probability that the loci are correctly identified (max = 1.0).</p

Sequence variants and allele distribution of genes in six rat MHC haplotypes.

a<p>All genes except <i>RT1-DMa</i> and <i>Tapbp</i> are encoded within the <i>Tcs2</i> locus.</p>b<p>Size of the coding sequences (cds) in bp.</p>c<p>Total number of synonymous and nonsynonymous SNPs in the cds.</p>d<p>Total number of insertions/deletions on gene level.</p>e<p>Allele distribution between MHC haplotypes; DA (RT1^a), DA.1F (RT1^f), DA.1I (RT1ⁱ), DA.1H (RT1^h), DA.1U (RT1^u). Sequence information for RT1ⁿ was obtained from NCBI GenBank.</p>f<p>Total number of amino acid substitutions.</p>g<p>Number of alternative isoforms at the protein level.</p>h<p><i>Btnl2</i> was analyzed in only two strains.</p>I<p>Sequence information for RT1-DOb refers to the full-length transcript of this gene (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004151#pgen.1004151.s001" target="_blank">Fig. S1</a> for alternative transcript).</p

Transcriptional regulation of MHC class I genes.

<p>(A) Allele-specific primers (Prim.) used for quantitative RT-PCR showed minimal amplification of other RT1-A alleles and were therefore considered class Ia-specific (i.e. not cross-reacting to class Ib genes); PCR products (Prod.) are shown for DA (<i>a</i>), DA.1UR83 (<i>u</i>), DA.1HR83 (<i>h</i>) and DA.1IR85 (<i>i</i>). (B) Expression in spleen of RT1-A genes in DA and <i>Tcs1</i>-congenic strains relative to the expression of beta-2-microglobuline (<i>B2M</i>). (C) Variation (fold-change) in RT1-A gene expression between different congenic strains. Data show the mean expression of 2–4 different primer sets per target gene after normalization to 3 reference genes (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004151#pgen.1004151.s014" target="_blank">Table S3</a>). Significant differences compared to <i>RT1-A^a</i>. (D) Expression of <i>RT1-A^a</i> (class Ia) and <i>clone 3.6</i> (class Ib) in spleen from DA rats. The amplification of a product in DA.1HR83 but not in DA.1H (1H) indicates that the primers for <i>clone 3.6</i> are not cross-reacting to the <i>RT1-A^a</i> gene in DA (adjacent figure).</p

Classical cim influences the number of CD8 recent thymic emigrants.

<p>(A) Recent thymic emigrants (RTEs) express CD90 and low levels of CD45RC. Counterplots show CD8 RTEs (gated) in the spleen of 6.5-week old congenic and DA rats (representative samples, numbers in gates show percent of CD8 T cells). Scatterplot shows total number of CD8 RTEs per spleen, which correlates to the absolute number of CD8SP TCR^hi cells in the thymi from the same animals (far right). (B) The corresponding staining (as shown in A) for CD4 RTEs, including total numbers (scatterplot) and correlation to CD4SP TCR^hi cells in the thymus. (C) Absolute numbers of CD4 (blue) and CD8 (red) T cells in the spleen of 13-week old rats. Strains and genotypes are shown above and below graphs, respectively; <i>n</i> = 5–7 per group.</p