11 research outputs found
Information included in the meeting minutes of the 20 EFCs (<i>n</i> = 160 reports).
<p>Information included in the meeting minutes of the 20 EFCs (<i>n</i> = 160 reports).</p
Comparison of characteristics of EFCs according to the number of actions decided in the year (< 5 versus ≥ 5).
<p>Comparison of characteristics of EFCs according to the number of actions decided in the year (< 5 versus ≥ 5).</p
Characteristics of analysis reports reported to committee (<i>n</i> = 91).
<p>Characteristics of analysis reports reported to committee (<i>n</i> = 91).</p
Characteristics of the actions adopted by the committee (<i>n</i> = 206).
<p>Characteristics of the actions adopted by the committee (<i>n</i> = 206).</p
Characteristics of event reports reviewed by EFCs (<i>n</i> = 1707).
<p>Characteristics of event reports reviewed by EFCs (<i>n</i> = 1707).</p
Patient 3 HCV alignments
6 HCV sequence alignments for patient 3 - Core and NS5B regions, 3 time sample
Patient 2 HVC alignments
10 HCV sequence alignments for patient 2 - Core and NS5B regions, 5 time samples
Additional file 5: Figure S4. of New insights into HCV replication in original cells from Aedes mosquitoes
HCV Infection protocols. (A) for human HepaRG hepatocytes (“H”) and (B) for insect cells, Ktmos1 (“K”, Ae Aegypti) and C6/36 (“C”, Ae Albopictus). The infection was performed using HCVsp, LAT isolate, genotype 3. D, day; − before infection; D0, day of infection; D4, D7, D14, D21, D28, days post-infection and medium change. P17, P18, passages 17 and 18. HepaRG®, HepaRG cells from KIT902 (Biopredic International). Over the time, HepaRG and Ktmos1 cells in monolayer became more and more differentiated. (TIFF 300 kb
Additional file 7: Figure S6. of New insights into HCV replication in original cells from Aedes mosquitoes
Absence of HCV RNA detection in HEK 293 cells. Cells were collected at days 0 (D0), 4 (D4), 21 (D21) and 28 (D28) p.i. The inoculum HCVsp (LAT isolate, genotype 3) was used as positive control. Non-infected (mock) cells (â) and HCV-infected (+) HEK 293 cells. (TIFF 1236 kb
Additional file 3: Figure S2. of New insights into HCV replication in original cells from Aedes mosquitoes
Fluorescence observation of adherent Ktmos1 cells. The Ktmos1 Aedes aegypti cells were grown on thin glass (0,17 mm), 2 chambers LabTek (Nunc). The cells were fixed after different periods of cultivation with 2% PFA for 20 min at 37 °C. After permeabilization by PBS containing 0,1% Triton X100 for 2 min, the nuclei were stained by Hoechst 33,258 (Sigma). Observation was performed on motorized inverted Olympus IE81 microscope using the DIC (Differential Interference Contrast) and the DAPI filter. The panel (A) shows a late metaphase stage of a dividing cell. The panel (B) shows Ktmos1 cells in monolayer. (TIFF 925 kb